scholarly journals Identification of Defense Compounds in Barbarea vulgaris against the Herbivore Phyllotreta nemorum by an Ecometabolomic Approach

2009 ◽  
Vol 151 (4) ◽  
pp. 1977-1990 ◽  
Author(s):  
Vera Kuzina ◽  
Claus Thorn Ekstrøm ◽  
Sven Bode Andersen ◽  
Jens Kvist Nielsen ◽  
Carl Erik Olsen ◽  
...  
Keyword(s):  
Barbarea Vulgaris ◽  
Defense Compounds ◽  
Phyllotreta Nemorum

scholarly journals AFLP markers for the R-gene in the flea beetle, Phyllotreta nemorum, conferring resistance to defenses in Barbarea vulgaris

2005 ◽  
Vol 5 (1) ◽  
Author(s):  
Casper J. Breuker ◽  
Kathleen Victoir ◽  
Peter W. De Jong ◽  
Eddy van der Meijden ◽  
Paul M. Brakefield ◽  
...  
Keyword(s):  
Flea Beetle ◽  
Aflp Markers ◽  
R Gene ◽  
Barbarea Vulgaris ◽  
Phyllotreta Nemorum

Barbarea vulgaris linkage map and quantitative trait loci for saponins, glucosinolates, hairiness and resistance to the herbivore Phyllotreta nemorum

2011 ◽  
Vol 72 (2-3) ◽  
pp. 188-198 ◽  
Author(s):  
Vera Kuzina ◽  
Jens Kvist Nielsen ◽  
Jörg Manfred Augustin ◽  
Anna Maria Torp ◽  
Søren Bak ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Linkage Map ◽  
Quantitative Trait ◽  
Barbarea Vulgaris ◽  
Trait Loci ◽  
Phyllotreta Nemorum

Pleiotropic effects associated with an allele enabling the flea beetle Phyllotreta nemorum to use Barbarea vulgaris as a host plant

2006 ◽  
Vol 21 (1) ◽  
pp. 13-26 ◽  
Author(s):  
Casper J. Breuker ◽  
Peter W. de Jong ◽  
Kathleen Victoir ◽  
Klaas Vrieling ◽  
Paul M. Brakefield
Keyword(s):  
Host Plant ◽  
Flea Beetle ◽  
Pleiotropic Effects ◽  
Barbarea Vulgaris ◽  
Phyllotreta Nemorum

scholarly journals Engineering and optimization of the 2-phenylethylglucosinolate production in Nicotiana benthamiana by combining biosynthetic genes from Barbarea vulgaris and Arabidopsis thaliana

2020 ◽  
Author(s):  
Cuiwei Wang ◽  
Christoph Crocoll ◽  
Niels Agerbirk ◽  
Barbara Ann Halkier
Keyword(s):  
Arabidopsis Thaliana ◽  
Nicotiana Benthamiana ◽  
Biosynthetic Pathway ◽  
Fold Increase ◽  
Chain Elongation ◽  
Heterologous Host ◽  
Barbarea Vulgaris ◽  
Donor Plant ◽  
Defense Compounds ◽  
Novel Approach

AbstractAmong the glucosinolate (GLS) defense compounds characteristic of the Brassicales order, several have been shown to promote human health. This includes 2-phenylethylglucosinolate (2PE) derived from homophenylalanine (HPhe). In this study, we used transient expression in Nicotiana benthamiana to validate and characterize previously predicted key genes in the 2PE biosynthetic pathway from Barbarea vulgaris and demonstrate the feasibility of engineering 2PE production. We used genes from B. vulgaris and Arabidopsis thaliana, in which the biosynthesis of GLSs is predominantly derived from HPhe and dihomomethionine, respectively. The resulting GLS profiles partially mirrored GLS profiles in the gene donor plant, but in both cases the profiles in N. benthamiana were wider than in the native plants. We found that BvBCAT4 is a more efficient entry enzyme for biosynthesis of both HPhe and dihomomethionine and that MAM1 enzymes determine the chain-elongated profile. Co-expression of the chain elongation pathway and CYP79F6 from B. vulgaris with the remaining aliphatic GLS core pathway genes from A. thaliana, demonstrated the feasibility of engineering production of 2PE in N. benthamiana. Noticeably, the HPhe-converting enzyme BvCYP79F6 in the core GLS pathway belongs to the CYP79F subfamily, a family believed to have substrate specificity towards chain-elongated methionine derivatives. Replacing the B. vulgaris chain elongation pathway with a chimeric pathway consisting of BvBCAT4, BvMAM1, AtIPMI and AtIPMDH1 resulted in an additional 2-fold increase in 2PE production, demonstrating that chimeric pathway with genes from different species can increase flux and boost production in an engineered pathway. Our study provides a novel approach to produce the important HPhe and 2PE in a heterologous host. Chimeric engineering of a complex biosynthetic pathway enabled detailed understanding of catalytic properties of individual enzymes - a prerequisite for understanding biochemical evolution - and with biotechnological and plant breeding potentials of new-to-nature gene combinations.

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