scholarly journals Regulation of Photosynthetic Induction State in High- and Low-Light-Grown Soybean and Alocasia macrorrhiza (L.) G. Don

1995 ◽  
Vol 109 (1) ◽  
pp. 307-317 ◽  
Author(s):  
J. P. Krall ◽  
E. V. Sheveleva ◽  
R. W. Pearcy
2017 ◽  
Vol 68 (20) ◽  
pp. 5629-5640 ◽  
Author(s):  
Elias Kaiser ◽  
Dianfan Zhou ◽  
Ep Heuvelink ◽  
Jeremy Harbinson ◽  
Alejandro Morales ◽  
...  

1988 ◽  
Vol 15 (2) ◽  
pp. 223 ◽  
Author(s):  
RW Pearcy

The light environment in forest understories is highly dynamic because the weak shade light is period- ically punctuated by lightflecks lasting from a second or less to tens of minutes. Although present for only a small fraction of the day, these lightflecks can contribute more than two-thirds of the photosynthetically active radiation. Several factots are of importance in determining the capacity of a leaf to utilise lightflecks. Following long low-light periods the induction state of the photosynthetic apparatus is limiting. During induction, 20-60 min may be required before maximum assimilation rates are reached due first to a light activation requirement. of ribulose-1,5-bisphosphate carboxylasel oxygenase and later to the light-induced stomatal opening. Continuous light is not required and induction occurring during a series of lightflecks results in higher carbon gain for later as compared to earlier lightflecks. Post-illumination CO2 fixation resulting from utilisation of metabolite pools built up during the lightfleck can significantly enhance carbon gain during short (5-20 s) lightflecks. The carbon gain of a leaf in response to a lightfleck is a consequence of the limitations imposed by induction state plus the enhancements due to post-illumination CO2 fixation. In the field, this will depend on the frequency and duration of the lightflecks and the duration of the intervening low-light periods.


Author(s):  
G.Y. Fan ◽  
J.M. Cowley

In recent developments, the ASU HB5 has been modified so that the timing, positioning, and scanning of the finely focused electron probe can be entirely controlled by a host computer. This made the asynchronized handshake possible between the HB5 STEM and the image processing system which consists of host computer (PDP 11/34), DeAnza image processor (IP 5000) which is interfaced with a low-light level TV camera, array processor (AP 400) and various peripheral devices. This greatly facilitates the pattern recognition technique initiated by Monosmith and Cowley. Software called NANHB5 is under development which, instead of employing a set of photo-diodes to detect strong spots on a TV screen, uses various software techniques including on-line fast Fourier transform (FFT) to recognize patterns of greater complexity, taking advantage of the sophistication of our image processing system and the flexibility of computer software.


Author(s):  
W. Lin ◽  
J. Gregorio ◽  
T.J. Holmes ◽  
D. H. Szarowski ◽  
J.N. Turner

A low-light level video microscope with long working distance objective lenses has been built as part of our integrated three-dimensional (3-D) light microscopy workstation (Fig. 1). It allows the observation of living specimens under sufficiently low light illumination that no significant photobleaching or alternation of specimen physiology is produced. The improved image quality, depth discrimination and 3-D reconstruction provides a versatile intermediate resolution system that replaces the commonly used dissection microscope for initial image recording and positioning of microelectrodes for neurobiology. A 3-D image is displayed on-line to guide the execution of complex experiments. An image composed of 40 optical sections requires 7 minutes to process and display a stereo pair.The low-light level video microscope utilizes long working distance objective lenses from Mitutoyo (10X, 0.28NA, 37 mm working distance; 20X, 0.42NA, 20 mm working distance; 50X, 0.42NA, 20 mm working distance). They provide enough working distance to allow the placement of microelectrodes in the specimen.


Author(s):  
Ray Keller

The amphibian embryo offers advantages of size, availability, and ease of use with both microsurgical and molecular methods in the analysis of fundamental developmental and cell biological problems. However, conventional wisdom holds that the opacity of this embryo limits the use of methods in optical microscopy to resolve the cell motility underlying the major shape-generating processes in early development.These difficulties have been circumvented by refining and adapting several methods. First, methods of explanting and culturing tissues were developed that expose the deep, nonepithelial cells, as well as the superficial epithelial cells, to the view of the microscope. Second, low angle epi-illumination with video image processing and recording was used to follow patterns of cell movement in large populations of cells. Lastly, cells were labeled with vital, fluorescent dyes, and their behavior recorded, using low-light, fluorescence microscopy and image processing. Using these methods, the details of the cellular protrusive activity that drives the powerful convergence (narrowing)


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