scholarly journals Pseudomonas fluorescens WCS374r-Induced Systemic Resistance in Rice against Magnaporthe oryzae Is Based on Pseudobactin-Mediated Priming for a Salicylic Acid-Repressible Multifaceted Defense Response

2008 ◽  
Vol 148 (4) ◽  
pp. 1996-2012 ◽  
Author(s):  
David De Vleesschauwer ◽  
Mohammad Djavaheri ◽  
Peter A.H.M. Bakker ◽  
Monica Höfte
Keyword(s):  
Salicylic Acid ◽  
Pseudomonas Fluorescens ◽  
Magnaporthe Oryzae ◽  
Defense Response ◽  
Induced Systemic Resistance ◽  
Systemic Resistance ◽  
Mediated Priming

scholarly journals Identification of miRNAs Involved in Bacillus velezensis FZB42-Activated Induced Systemic Resistance in Maize

2019 ◽  
Vol 20 (20) ◽  
pp. 5057 ◽  
Author(s):  
Shanshan Xie ◽  
Hengguo Yu ◽  
Enze Li ◽  
Yu Wang ◽  
Juan Liu ◽  
...  
Keyword(s):  
Defense Response ◽  
Target Genes ◽  
Induced Systemic Resistance ◽  
Systemic Resistance ◽  
Abiotic Stress Response ◽  
Bacillus Velezensis ◽  
Nuclear Factor Y ◽  
Network Analyses ◽  
Cis Element ◽  
Differentially Expressed Mirna

Bacillus velezensis FZB42 is able to activate induced systemic resistance (ISR) to enhance plant defense response against pathogen infections. Though the roles of microRNAs (miRNAs) in Bacillus-triggered ISR have been reported in Arabidopsis, the maize miRNAs responsible for the Bacillus-activated ISR process have not been discovered. To explore the maize miRNAs involved in ISR, maize miRNAs in response to FZB42 (ISR activating), FZB42△sfp△alss (deficient in triggering ISR), and a control for 12 h were sequenced. A total of 146 known miRNAs belonging to 30 miRNA families and 217 novel miRNAs were identified. Four miRNAs specifically repressed in FZB42-treatment were selected as candidate ISR-associated miRNAs. All of them contained at least one defense response-related cis-element, suggesting their potential roles in activating the ISR process. Interestingly, three of the four candidate ISR-associated miRNAs belong to the conserved miR169 family, which has previously been confirmed to play roles in abiotic stress response. Moreover, 52 mRNAs were predicted as potential targets of these candidate ISR-associated miRNAs through TargetFinder software and degradome sequencing. Gene Ontology (GO) and network analyses of target genes showed that these differentially expressed miRNA might participate in the ISR process by regulating nuclear factor Y transcription factor. This study is helpful in better understanding the regulatory roles of maize miRNAs in the Bacillus-activated ISR process.

scholarly journals Induced Systemic Resistance in Arabidopsis thaliana Against Pseudomonas syringae pv. tomato by 2,4-Diacetylphloroglucinol-Producing Pseudomonas fluorescens

2012 ◽  
Vol 102 (4) ◽  
pp. 403-412 ◽  
Author(s):  
David M. Weller ◽  
Dmitri V. Mavrodi ◽  
Johan A. van Pelt ◽  
Corné M. J. Pieterse ◽  
Leendert C. van Loon ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Pseudomonas Fluorescens ◽  
Induced Resistance ◽  
Pseudomonas Syringae ◽  
Induced Systemic Resistance ◽  
Signal Transduction Pathway ◽  
Systemic Resistance ◽  
Wild Type ◽  
Challenge Inoculation ◽  
Dependent Signal

Pseudomonas fluorescens strains that produce the polyketide antibiotic 2,4-diacetylphloroglucinol (2,4-DAPG) are among the most effective rhizobacteria that suppress root and crown rots, wilts, and damping-off diseases of a variety of crops, and they play a key role in the natural suppressiveness of some soils to certain soilborne pathogens. Root colonization by 2,4-DAPG-producing P. fluorescens strains Pf-5 (genotype A), Q2-87 (genotype B), Q8r1-96 (genotype D), and HT5-1 (genotype N) produced induced systemic resistance (ISR) in Arabidopsis thaliana accession Col-0 against bacterial speck caused by P. syringae pv. tomato. The ISR-eliciting activity of the four bacterial genotypes was similar, and all genotypes were equivalent in activity to the well-characterized strain P. fluorescens WCS417r. The 2,4-DAPG biosynthetic locus consists of the genes phlHGF and phlACBDE. phlD or phlBC mutants of Q2-87 (2,4-DAPG minus) were significantly reduced in ISR activity, and genetic complementation of the mutants restored ISR activity back to wild-type levels. A phlF regulatory mutant (overproducer of 2,4-DAPG) had ISR activity equivalent to the wild-type Q2-87. Introduction of DAPG into soil at concentrations of 10 to 250 μM 4 days before challenge inoculation induced resistance equivalent to or better than the bacteria. Strain Q2-87 induced resistance on transgenic NahG plants but not on npr1-1, jar1, and etr1 Arabidopsis mutants. These results indicate that the antibiotic 2,4-DAPG is a major determinant of ISR in 2,4-DAPG-producing P. fluorescens, that the genotype of the strain does not affect its ISR activity, and that the activity induced by these bacteria operates through the ethylene- and jasmonic acid-dependent signal transduction pathway.

scholarly journals Induced Systemic Resistance in Arabidopsis thaliana in Response to Root Inoculation with Pseudomonas fluorescens CHA0

2003 ◽  
Vol 16 (10) ◽  
pp. 851-858 ◽  
Author(s):  
Annalisa Iavicoli ◽  
Emmanuel Boutet ◽  
Antony Buchala ◽  
Jean-Pierre Métraux
Keyword(s):  
Arabidopsis Thaliana ◽  
Jasmonic Acid ◽  
Pseudomonas Fluorescens ◽  
Transgenic Line ◽  
Induced Systemic Resistance ◽  
Antibiotic Activity ◽  
Systemic Resistance ◽  
Peronospora Parasitica ◽  
Molecular Processes ◽  
Molecular Determinants

Root inoculation of Arabidopsis thaliana ecotype Columbia with Pseudomonas fluorescens CHA0r partially protected leaves from the oomycete Peronospora parasitica. The molecular determinants of Pseudomonas fluorescens CHA0r for this induced systemic resistance (ISR) were investigated, using mutants derived from strain CHA0: CHA400 (pyoverdine deficient), CHA805 (exoprotease deficient), CHA77 (HCN deficient), CHA660 (pyoluteorin deficient), CHA631 (2,4-diacetylphloroglucinol [DAPG] deficient), and CHA89 (HCN, DAPG- and pyoluteorin deficient). Only mutations interfering with DAPG production led to a significant decrease in ISR to Peronospora parasitica. Thus, DAPG production in Pseudomonas fluorescens is required for the induction of ISR to Peronospora parasitica. DAPG is known for its antibiotic activity; however, our data indicate that one action of DAPG could be due to an effect on the physiology of the plant. DAPG at 10 to 100 μM applied to roots of Arabidopsis mimicked the ISR effect. CHA0r-mediated ISR was also tested in various Arabidopsis mutants and transgenic plants: NahG (transgenic line degrading salicylic acid [SA]), sid2-1 (nonproducing SA), npr1-1 (non-expressing NPR1 protein), jar1-1 (insensitive to jasmonic acid and methyl jasmonic acid), ein2-1 (insensitive to ethylene), etr1-1 (insensitive to ethylene), eir1-1 (insensitive to ethylene in roots), and pad2-1 (phytoalexin deficient). Only jar1-1, eir1-1, and npr1-1 mutants were unable to undergo ISR. Sensitivity to jasmonic acid and functional NPR1 and EIR1 proteins were required for full expression of CHA0r-mediated ISR. The requirements for ISR observed in this study in Peronospora parasitica induced by Pseudomonas fluorescens CHA0r only partially overlap with those published so far for Peronospora parasitica, indicating a great degree of flexibility in the molecular processes leading to ISR.

scholarly journals Role of salicylic acid in systemic resistance induced by Pseudomonas fluorescens against Fusarium oxysporum f. sp. ciceri in chickpea

2003 ◽  
Vol 158 (3) ◽  
pp. 203-213 ◽  
Author(s):  
Ratul Saikia ◽  
Tanuja Singh ◽  
Rakesh Kumar ◽  
Juhi Srivastava ◽  
Alok K. Srivastava ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Fusarium Oxysporum ◽  
Pseudomonas Fluorescens ◽  
Systemic Resistance

scholarly journals Salicylic Acid Produced by Serratia marcescens 90-166 Is Not the Primary Determinant of Induced Systemic Resistance in Cucumber or Tobacco

1997 ◽  
Vol 10 (6) ◽  
pp. 761-768 ◽  
Author(s):  
C. M. Press ◽  
M. Wilson ◽  
S. Tuzun ◽  
J. W. Kloepper
Keyword(s):  
Salicylic Acid ◽  
Serratia Marcescens ◽  
Pseudomonas Syringae ◽  
Ferric Iron ◽  
Induced Systemic Resistance ◽  
Iron Concentration ◽  
Systemic Resistance ◽  
Broth Culture ◽  
In Planta

The rhizobacterial strain Serratia marcescens 90–66 mediates induced systemic resistance (ISR) to fungal, bacterial, and viral pathogens. It was determined that strain 90–166 produced salicylic acid (SA), using the salicylateresponsive reporter plasmid pUTK21. High-pressure liquid chromatography analysis of culture extracts confirmedthe production of SA in broth culture. Mini-Tn5phoA mutants, which did not produce detectable amounts of SA, retained ISR activity in cucumber against the fungal pathogen Colletotrichum orbiculare. Strain 90–166 induced disease resistance to Pseudomonas syringae pv. tabaci in wild-type Xanthi-nc and transgenic NahG-10 tobacco expressing salicylate hydroxylase. Increasing ferric iron concentrations in vitro reduced SA production below detectable limits, and increasing ferric iron concentration in planta, applied as a root drench, significantly reduced the level of ISR observed in cucumber to C. orbiculare. An ISR¯ mutant (90-166-2882) still produced SA. The results of this study indicate that SA produced by 90–166 is not the primary bacterial determinant of ISR and that this bacterial-mediated ISR system is affected by iron concentration.

scholarly journals No Role for Bacterially Produced Salicylic Acid in Rhizobacterial Induction of Systemic Resistance in Arabidopsis

2005 ◽  
Vol 95 (11) ◽  
pp. 1349-1355 ◽  
Author(s):  
L. X. Ran ◽  
L. C. van Loon ◽  
P. A. H. M. Bakker
Keyword(s):  
Salicylic Acid ◽  
Induced Systemic Resistance ◽  
Incubation Temperature ◽  
Growth Conditions ◽  
Systemic Resistance ◽  
Bacterial Speck ◽  
Fluorescent Pseudomonas ◽  
Pathogenesis Related Protein ◽  
Induction Of Resistance

The role of bacterially produced salicylic acid (SA) in the induction of systemic resistance in plants by rhizobacteria is far from clear. The strong SA producer Pseudomonas fluorescens WCS374r induces resistance in radish but not in Arabidopsis thaliana, whereas application of SA leads to induction of resistance in both plant species. In this study, we compared P. fluorescens WCS374r with three other SA-producing fluorescent Pseudomonas strains, P. fluorescens WCS417r and CHA0r, and P. aeruginosa 7NSK2 for their abilities to produce SA under different growth conditions and to induce systemic resistance in A. thaliana against bacterial speck, caused by P. syringae pv. tomato. All strains produced SA in vitro, varying from 5 fg cell-1 for WCS417r to >25 fg cell-1 for WCS374r. Addition of 200 μM FeCl3 to standard succinate medium abolished SA production in all strains. Whereas the incubation temperature did not affect SA production by WCS417r and 7NSK2, strains WCS374r and CHA0r produced more SA when grown at 33 instead of 28°C. WCS417r, CHA0r, and 7NSK2 induced systemic resistance apparently associated with their ability to produce SA, but WCS374r did not. Conversely, a mutant of 7NSK2 unable to produce SA still triggered induced systemic resistance (ISR). The possible involvement of SA in the induction of resistance was evaluated using SA-nonaccumulating transgenic NahG plants. Strains WCS417r, CHA0r, and 7NSK2 induced resistance in NahG Arabidopsis. Also, WCS374r, when grown at 33 or 36°C, triggered ISR in these plants, but not in ethylene-insensitive ein2 or in non-plant pathogenesis- related protein-expressing npr1 mutant plants, irrespective of the growth temperature of the bacteria. These results demonstrate that, whereas WCS374r can be manipulated to trigger ISR in Arabidopsis, SA is not the primary determinant for the induction of systemic resistance against bacterial speck disease by this bacterium. Also, for the other SAproducing strains used in this study, bacterial determinants other than SA must be responsible for inducing resistance.

Salicylic acid induced systemic resistance on onion plants againstStemphylium vesicarium

2009 ◽  
Vol 42 (11) ◽  
pp. 1042-1050 ◽  
Author(s):  
Kamal A. M. Abo-Elyousr ◽  
M. A. M. Hussein ◽  
A. D. A. Allam ◽  
M. H. Hassan
Keyword(s):  
Salicylic Acid ◽  
Induced Systemic Resistance ◽  
Systemic Resistance

Induced resistance and control of charcoal rot in Cicer arietinum (chickpea) by Pseudomonas fluorescens

2001 ◽  
Vol 79 (7) ◽  
pp. 787-795 ◽  
Author(s):  
Alok K Srivastava ◽  
Tanuja Singh ◽  
T K Jana ◽  
Dilip K Arora
Keyword(s):  
Pseudomonas Fluorescens ◽  
Cicer Arietinum ◽  
Induced Resistance ◽  
Time Course ◽  
Induced Systemic Resistance ◽  
Macrophomina Phaseolina ◽  
Systemic Resistance ◽  
Charcoal Rot ◽  
Chemical Inducers ◽  
Rot Disease

Pseudomonas fluorescens isolate 4-92 induced systemic resistance against charcoal rot disease in chickpea (Cicer arietinum L.) caused by Macrophomina phaseolina (Tassi) Goidanich. Time-course accumulation of pathogenesis-related (PR) proteins (chitinases and glucanases) in chickpea plants inoculated with P. fluorescens was significantly (P = 0.05) higher than in control plants. The level of chitinases and glucanases increased by 6.6- to 7-fold up to 4 days postinoculation; thereafter, little decrease in the activity of PR proteins was observed. Root-colonizing populations of P. fluorescens were at a maximum 2 days after transplantation at different inoculum concentrations, and decreased over time. Inoculation of root tips of chickpea by P. fluorescens, 2,6-dichloroisonicotinic acid, and o-acetylsalicylic acid induced systemic resistance against charcoal rot. Disease was 33 to 55.5% higher in control plants than in plants inoculated with chemical inducers or P. fluorescens. Single treatment of plants with P. fluorescens increased disease resistance by 33%, whereas combined application of P. fluorescens with either of the chemical inducers was most effective in inducing the resistance by 2- to 2.25-fold. The time-course study shows that an interval of at least 2 days was required between induction treatment and challenge inoculation. Biocontrol efficacy of P. fluorescens against charcoal rot disease in chickpea was demonstrated under greenhouse conditions.Key words: biological control, induced resistance, Macrophomina phaseolina, Pseudomonas fluorescens.

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