scholarly journals Proteome-Wide Characterization of Seed Aging in Arabidopsis: A Comparison between Artificial and Natural Aging Protocols

2008 ◽  
Vol 148 (1) ◽  
pp. 620-641 ◽  
Author(s):  
Loïc Rajjou ◽  
Yoann Lovigny ◽  
Steven P.C. Groot ◽  
Maya Belghazi ◽  
Claudette Job ◽  
...  
Keyword(s):  
2021 ◽  
Vol 7 (7) ◽  
pp. 559
Author(s):  
Yaping Wang ◽  
Yuhang Fu ◽  
Yuanyuan He ◽  
Muhammad Fakhar-e-Alam Kulyar ◽  
Mudassar Iqbal ◽  
...  

Development phases are important in maturing immune systems, intestinal functions, and metabolism for the construction, structure, and diversity of microbiome in the intestine during the entire life. Characterizing the gut microbiota colonization and succession based on age-dependent effects might be crucial if a microbiota-based therapeutic or disease prevention strategy is adopted. The purpose of this study was to reveal the dynamic distribution of intestinal bacterial and fungal communities across all development stages in yaks. Dynamic changes (a substantial difference) in the structure and composition ratio of the microbial community were observed in yaks that matched the natural aging process from juvenile to natural aging. This study included a significant shift in the abundance and proportion of bacterial phyla (Planctomycetes, Firmicutes, Bacteroidetes, Spirochaetes, Tenericutes, Proteobacteria, and Cyanobacteria) and fungal phyla (Chytridiomycota, Mortierellomycota, Neocallimastigomycota, Ascomycota, and Basidiomycota) across all development stages in yaks. As yaks grew older, variation reduced, and diversity increased as compared to young yaks. In addition, the intestine was colonized by a succession of microbiomes that coalesced into a more mature adult, including Ruminococcaceae_UCG-005, Romboutsia, Prevotellaceae_UCG-004, Blautia, Clostridium_sensu_stricto_1, Ruminococcus_1, Ruminiclostridium_5, Rikenellaceae_RC9_gut_group, Alloprevotella, Acetitomaculum, Lachnospiraceae_NK3A20_group, Bacteroides, Treponema_2, Olsenella, Escherichia-Shigella, Candidatus_Saccharimonas, and fungal communities Mortierella, Lomentospora, Orpinomyces, and Saccharomyces. In addition, microorganisms that threaten health, such as Escherichia-Shigella, Mortierella, Lomentospora and Hydrogenoanaerobacterium, Corynebacterium_1, Trichosporon, and Coprinellus, were enriched in young and old yaks, respectively, although all yaks were healthy. The significant shifts in microflora composition and structure might reflect adaptation of gut microbiome, which is associated with physicochemical conditions changes and substrate availability in the gut across all development periods of yaks.


PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e10569
Author(s):  
Arleta Małecka ◽  
Liliana Ciszewska ◽  
Aleksandra Staszak ◽  
Ewelina Ratajczak

Aging is one of the most fundamental biological processes occurring in all forms of eukaryotic life. Beech trees (Fagus sylvatica L.) produce seeds in intervals of 5–10 years. Its yearly seed yield is usually very low, so there is a need for long-term seed storage to enable propagation of this species upon demand. Seeds for sowing must be of high quality but they are not easy to store without viability loss. Understanding the mechanism responsible for seed aging is therefore very important. We observed the generation of reactive oxygen species (ROS) in mitochondria of embryonic axes and cotyledons of beech seeds during natural aging. The presence of ROS led to changes in compromised mitochondrial membrane integrity and in mitochondrial metabolism and morphology. In this study, we pointed to the involvement of mitochondria in the natural aging process of beech seeds, but the molecular mechanisms underlying this involvement are still unknown.


2012 ◽  
Vol 710 ◽  
pp. 174-179
Author(s):  
Niraj Nayan ◽  
S.V.S. Narayana Murty ◽  
S.C. Sharma ◽  
Parameshwar Prasad Sinha

High shear strength of 25 kg/mm2 combined with a high plasticity makes Russian grade V65 aluminum alloys as potential candidate compared to AA2024 for riveting applications in aerospace industries. The lower content of magnesium over the AA 2024 alloy decreases the aging kinetics of this alloy because of the decreased vacancy concentration in the as-quenched alloy which in turn gives flexibility to carryout riveting operation even after 4 days after solution heat treat¬ment and natural aging at room temperature. This paper presents the processing and characterization of V65 aluminium alloy wires processed from hot and warm rolled rods. Mechanical properties of the wires have been evaluated in T6 and T4 tempers and correlated with microstructures. Detailed microstructural examination using optical microscopy and fractography of the tensile tested samples using scanning electron microscopy were carriedout.


Author(s):  
Yu A Amelkovich ◽  
O B Nazarenko ◽  
A I Sechin ◽  
P M Visakh

2019 ◽  
Vol 126 (10) ◽  
pp. 105113 ◽  
Author(s):  
Byung Heon Han ◽  
Yoocheon Kim ◽  
Seung-gyo Jang ◽  
Jeayong Yoo ◽  
Jack J. Yoh

2016 ◽  
Vol 70 (5) ◽  
pp. 581-594 ◽  
Author(s):  
Nikola Gligorijevic ◽  
Vesna Rodic ◽  
Sasa Zivkovic ◽  
Bojan Pavkovic ◽  
Momcilo Nikolic ◽  
...  

This paper presents the procedure of uniaxial mechanical characterization of composite solid rocket propellant based on hydroxy-terminated polybutadiene (HTPB), whose mechanical properties strongly depend on temperature, strain rate, natural aging and accumulated damage. A method of processing data is presented in order to determine time-temperature shift factor and master curves for tensile strength, ultimate strain and relaxation modulus, depending on reduced time. Functional dependences of these features represent an input for structural analysis of a rocket motor propellant grain. The effects of natural aging on the mechanical properties are also considered.


2015 ◽  
Vol 830-831 ◽  
pp. 131-134 ◽  
Author(s):  
Bikramjit Podder ◽  
Chandan Mondal ◽  
G. Gopi ◽  
K. Ramesh Kumar

The present study reports the effect of the heat treatment on the tensile properties of the reverse flow-formed AA6082 Aluminum alloy tube. Tensile specimens obtained after each forming pass have been subjected to three different heat-treatment conditions viz., as-flow formed (AFF), as-flow formed followed by artificial aging (170°C/6h) and HT (solutionizing + 170°C/6h) treatments. Characterization of the tensile properties reveals that as-flow formed condition (followed by natural aging) gives the best combination of yield strength, UTS and percentage of elongation. The variations in tensile properties are correlated with microstructure of the materials.


2021 ◽  
pp. 096739112110476
Author(s):  
Cynthia DC Erbetta ◽  
Maria Elisa SR Silva ◽  
Roberto FS Freitas ◽  
Ricardo G Sousa

The study of the behavior of polymeric material used for insulators in the electrical system is extremely important in order to evaluate their lifetime as well as their performance when exposed to different environmental conditions. In the present work, the behavior of high-density polyethylene (HDPE) pin type insulators (15 kV), under accelerated aging conditions, was studied. Samples were exposed to aging, for 200 h, 1000 h, and 2000 h, in accelerated weathering chambers, according to two different methods. In Method 1, the parameters were established based on natural aging in location conditions, and in Method 2, ASTM G155 standard parameters were used. All samples were characterized by rheometry, Fourier-transform infrared spectroscopy (FTIR), and differential scanning calorimetry (DSC). The accelerated aging conditions, used in Methods 1 and 2, affected the aging of HDPE pin type insulators samples differently, the changes being more intense in Method 1.


Materials ◽  
2020 ◽  
Vol 13 (18) ◽  
pp. 4051
Author(s):  
Kyle Tsaknopoulos ◽  
Caitlin Walde ◽  
Derek Tsaknopoulos ◽  
Victor Champagne ◽  
Danielle Cote

Aluminum 5056 is a work-hardenable alloy known for its corrosion resistance with new applications in additive manufacturing. A good understanding of the secondary phases in Al 5056 powders is important for understanding the properties of the final parts. In this study, the effects of different thermal treatments on the microstructure of Al 5056 powder were studied. Thermodynamic models were used to guide the interpretation of the microstructure as a function of thermal treatment, providing insight into the stability of different possible phases present in the alloy. Through the use of transmission electron microscopy (TEM) and energy-dispersive X-ray spectroscopy (EDS), combined with thermodynamic modeling, a greater understanding of the internal microstructure of Al 5056 powder has been achieved in both the as-atomized and thermally treated conditions. Evidence of natural aging within these powders was observed, which speaks to the shelf-life of these powders and the importance of proper treatment and storage to maintain consistent results.


Author(s):  
B. L. Soloff ◽  
T. A. Rado

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.


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