scholarly journals A Symbiotic Plant Peroxidase Involved in Bacterial Invasion of the Tropical Legume Sesbania rostrata

2007 ◽  
Vol 144 (2) ◽  
pp. 717-727 ◽  
Author(s):  
Jeroen Den Herder ◽  
Sam Lievens ◽  
Stephane Rombauts ◽  
Marcelle Holsters ◽  
Sofie Goormachtig
Keyword(s):  
Bacterial Invasion ◽  
Sesbania Rostrata ◽  
Plant Peroxidase ◽  
Tropical Legume

Chalcone reductase-homologous transcripts accumulate during development of stem-borne nodules on the tropical legume Sesbania rostrata

Planta ◽  
1999 ◽  
Vol 209 (1) ◽  
pp. 45-52 ◽  
Author(s):  
Sofie Goormachtig ◽  
Sam Lievens ◽  
Sylvia Herman ◽  
Marc Van Montagu ◽  
Marcelle Holsters
Keyword(s):  
Sesbania Rostrata ◽  
Tropical Legume ◽  
Chalcone Reductase

Nodulation-enhanced sequences from the water stress-tolerant tropical legume Sesbania rostrata

Plant Science ◽  
2004 ◽  
Vol 167 (2) ◽  
pp. 207-216 ◽  
Author(s):  
Katrien Schroeyers ◽  
Cristian Chaparro ◽  
Sofie Goormachtig ◽  
Marcelle Holsters
Keyword(s):  
Water Stress ◽  
Sesbania Rostrata ◽  
Tropical Legume

scholarly journals Rhizobial Factors Required for Stem Nodule Maturation and Maintenance in Sesbania rostrata-Azorhizobium caulinodans ORS571 Symbiosis

2007 ◽  
Vol 73 (20) ◽  
pp. 6650-6659 ◽  
Author(s):  
Shino Suzuki ◽  
Toshihiro Aono ◽  
Kyung-Bum Lee ◽  
Tadahiro Suzuki ◽  
Chi-Te Liu ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Sesbania Rostrata ◽  
Azorhizobium Caulinodans ◽  
Physiological Mechanisms ◽  
Repeated Observations ◽  
Stem Nodule ◽  
Tropical Legume ◽  
Fixation Activity ◽  
Stem Nodulation ◽  
Azorhizobium Caulinodans Ors571

ABSTRACT The molecular and physiological mechanisms behind the maturation and maintenance of N2-fixing nodules during development of symbiosis between rhizobia and legumes still remain unclear, although the early events of symbiosis are relatively well understood. Azorhizobium caulinodans ORS571 is a microsymbiont of the tropical legume Sesbania rostrata, forming N2-fixing nodules not only on the roots but also on the stems. In this study, 10,080 transposon-inserted mutants of A. caulinodans ORS571 were individually inoculated onto the stems of S. rostrata, and those mutants that induced ineffective stem nodules, as displayed by halted development at various stages, were selected. From repeated observations on stem nodulation, 108 Tn5 mutants were selected and categorized into seven nodulation types based on size and N2 fixation activity. Tn5 insertions of some mutants were found in the well-known nodulation, nitrogen fixation, and symbiosis-related genes, such as nod, nif, and fix, respectively, lipopolysaccharide synthesis-related genes, C4 metabolism-related genes, and so on. However, other genes have not been reported to have roles in legume-rhizobium symbiosis. The list of newly identified symbiosis-related genes will present clues to aid in understanding the maturation and maintenance mechanisms of nodules.

scholarly journals Functional nodFE Genes Are Present in Sinorhizobium sp. Strain MUS10, a Symbiont of the Tropical Legume Sesbania rostrata

2008 ◽  
Vol 74 (9) ◽  
pp. 2921-2923 ◽  
Author(s):  
Hari B. Krishnan ◽  
Demosthenis Chronis
Keyword(s):  
Sequence Homology ◽  
Acyl Carrier Protein ◽  
Sesbania Rostrata ◽  
Carrier Protein ◽  
Tropical Legume

ABSTRACT We have cloned the nodFE operon from Sinorhizobium sp. strain MUS10. MUS10 NodF shows sequence homology to acyl carrier protein and enables an S. meliloti nodF mutant to effectively nodulate alfalfa. Our results demonstrate the occurrence of nodFE in a symbiont that nodulates a legume host not belonging to the galegoid group.

scholarly journals Expression of Cell Cycle Genes During Sesbania rostrata Stem Nodule Development

1997 ◽  
Vol 10 (3) ◽  
pp. 316-325 ◽  
Author(s):  
Sofie Goormachtig ◽  
Marcio Alves-Ferreira ◽  
Marc Van Montagu ◽  
Gilbert Engler ◽  
Marcelle Holsters
Keyword(s):  
Root Meristem ◽  
Expression Patterns ◽  
Nodule Development ◽  
Bacterial Invasion ◽  
Sesbania Rostrata ◽  
Histone H4 ◽  
Root Primordia ◽  
Kinase Gene ◽  
Stem Nodule ◽  
Infected Root

Upon infection of Sesbania rostrata with Azorhizobium caulinodans, nodules are formed on roots and stems. Stem nodules develop from abundantly distributed dormant root primordia. To acquire more insight into the meristem organization during stem nodule development, the expression patterns of a mitotic B1-type cyclin gene (Sesro; CycB1;1), a cyclin-dependent kinase gene (Cdc2-1Sr), and a histone H4 gene (H4-1Sr) of S. rostrata were followed by in situ hybridization. Cdc2-1Sr transcripts were found in all cells of uninfected and infected root primordia. In uninfected root primordia, Sesro;CycB1;1 transcripts were detected in a few cells of the apical root meristem whereas H4-1Sr transcripts were abundant in this region. Interestingly, after inoculation with A. caulinodans, H4-1Sr transcripts disappeared in the root meristem and a patchy pattern of Sesro;CycB1;1 and H4-1Sr expression appeared in the cortex of the root primordium, reflecting the formation of globular nodule primordia. When bacterial invasion started, a distal nodule meristem was delimited wherein Sesro;CycB1;1 and H4-1Sr expression was concentrated. Approximately 1 week after inoculation, meristem activity ceased, indicated by the loss of Sesro;CycB1;1 and H4-1Sr expression.

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