scholarly journals Identification of a Bipartite Jasmonate-Responsive Promoter Element in the Catharanthus roseus ORCA3 Transcription Factor Gene That Interacts Specifically with AT-Hook DNA-Binding Proteins

2007 ◽  
Vol 144 (3) ◽  
pp. 1680-1689 ◽  
Author(s):  
Débora Vom Endt ◽  
Marina Soares e Silva ◽  
Jan W. Kijne ◽  
Giancarlo Pasquali ◽  
Johan Memelink
Keyword(s):  
Transcription Factor ◽  
Dna Binding ◽  
Catharanthus Roseus ◽  
Binding Proteins ◽  
Dna Binding Proteins ◽  
Promoter Element ◽  
Transcription Factor Gene ◽  
Factor Gene

scholarly journals THE CHARACTERISTICS OF MIRNA BINDING SITES IN MRNA OF ZFHX3 GENE AND ITS ORTHOLOGS

2018 ◽  
Vol 22 (4) ◽  
pp. 438-444 ◽  
Author(s):  
A. М. Kondybayeva ◽  
A. N. Akimniyazova ◽  
S. U. Kamenova ◽  
A. Т. Ivashchenko
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Dna Binding ◽  
Binding Sites ◽  
Animal Species ◽  
Biological Function ◽  
Dna Binding Proteins ◽  
Trinucleotide Repeats ◽  
Transcription Factor Gene ◽  
Factor Gene

Transcription factor gene ZFHX3 is one of the candidate genes involved in stroke development. The ZFHX3 protein contains oligopeptides encoded by trinucleotide repeats (TNRs). TNR variability is considered to be one of the causes of the disease, but their biological function has not yet been established. We assume that TNRs are the binding sites of miRNA to mRNA and are involved in regulation of ZFHX3 gene expression. The characteristics of miRNA–mRNA interaction were determined using MirTarget software. It has been shown that the first TNR in mRNA of the human ZFHX3 gene consists of the seven consecutive miR-12-32603-3p binding encoding polyGlu. The ZFHX3 protein of human polyGlu contains 30 Glu. In the orthologous proteins of 36 animal species the length of polyGlu varied from 27 Glu to 33 Glu. Negatively charged polyGlu of the ZFHX3 transcription factor probably interacted with positive DNA-binding proteins. The following mRNA region of the ZFHX3 gene contained the binding sites for miR-17-39416-3p, miR-5-15733-3p, miR-9-20317-3 encoding polyAla by 15 Ala lengths. In the 33 ZFHX3 orthologous proteins polyAla had the same length. The mRNA region of the human ZFHX3 gene with binding polysite of miR-1322-3p encoded polyGln consisting of 19 Gln. In the 41 orthologs of the ZFHX3 protein the length of polyGln varied from seven Gln to 23 Gln. The binding sites of miR-2-6184-3p, miR-5-14114-5p and miR-19-43437-5p were located with overlapping nucleotides sequences, and encode polyPro. In ZFHX3 human polyPro consisted of 12 Pro. In the orthologs, polyPro contained from 10 Pro to 14 Pro. The binding sites of miR-17-39416-3p, miR-9-20317-3p, miR-1-1819-3p, miR-5-15733-3p, miR-6-17815-3p, miR-18-39953-5p, miR-26862-5p, miR-1260b and miR-X-48174-3p in human ZFHX3 encoded polyGly by 22 Gly length. In the 28 orthologs of ZFHX3 the length of polyGly decreased to 11 Gly. The TNR regions could simultaneously bind several miRNAs, which increased the dependence of gene expression on miRNA. The oligopeptides encoded by the binding polysites of miRNA in mRNA in the orthologous ZFHX3 proteins were flanked by conserved oligopeptides.

scholarly journals Regulation of Transcription by Hypoxia Requires a Multiprotein Complex That Includes Hypoxia-Inducible Factor 1, an Adjacent Transcription Factor, and p300/CREB Binding Protein

1998 ◽  
Vol 18 (7) ◽  
pp. 4089-4096 ◽  
Author(s):  
Benjamin L. Ebert ◽  
H. Franklin Bunn
Keyword(s):  
Transcription Factor ◽  
Transcription Factors ◽  
Dna Binding ◽  
Binding Proteins ◽  
Binding Protein ◽  
Hypoxia Inducible Factor ◽  
Dna Binding Proteins ◽  
Creb Binding Protein ◽  
Multiprotein Complex ◽  
Hypoxia Inducible Factor 1

ABSTRACT Molecular adaptation to hypoxia depends on the binding of hypoxia-inducible factor 1 (HIF-1) to cognate response elements in oxygen-regulated genes. In addition, adjacent sequences are required for hypoxia-inducible transcription. To investigate the mechanism of interaction between these cis-acting sequences, the multiprotein complex binding to the lactate dehydrogenase A (LDH-A) promoter was characterized. The involvement of HIF-1, CREB-1/ATF-1, and p300/CREB binding protein (CBP) was demonstrated by techniques documenting in vitro binding, in combination with transient transfections that test the in vivo functional importance of each protein. In both the LDH-A promoter and the erythropoietin 3′ enhancer, formation of multiprotein complexes was analyzed by using biotinylated probes encompassing functionally critical cis-acting sequences. Strong binding of p300/CBP required interactions with multiple DNA binding proteins. Thus, the necessity of transcription factor binding sites adjacent to a HIF-1 site for hypoxically inducible transcription may be due to the requirement of p300 to interact with multiple transcription factors for high-affinity binding and activation of transcription. Since it has been found to interact with a wide range of transcription factors, p300 is likely to play a similar role in other genes, mediating interactions between DNA binding proteins, thereby activating stimulus-specific and tissue-specific gene transcription.

scholarly journals A family of genus-specific RNAs in tandem with DNA-binding proteins control expression of thebadAmajor virulence factor gene inBartonella henselae

2016 ◽  
Vol 6 (2) ◽  
pp. e00420 ◽  
Author(s):  
Nhan Tu ◽  
Ronan K. Carroll ◽  
Andy Weiss ◽  
Lindsey N. Shaw ◽  
Gael Nicolas ◽  
...  
Keyword(s):  
Dna Binding ◽  
Virulence Factor ◽  
Binding Proteins ◽  
Dna Binding Proteins ◽  
Factor Gene ◽  
Virulence Factor Gene

scholarly journals Differential display of DNA-binding proteins reveals heat-shock factor 1 as a circadian transcription factor

2008 ◽  
Vol 22 (3) ◽  
pp. 331-345 ◽  
Author(s):  
H. Reinke ◽  
C. Saini ◽  
F. Fleury-Olela ◽  
C. Dibner ◽  
I. J. Benjamin ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Heat Shock ◽  
Dna Binding ◽  
Differential Display ◽  
Binding Proteins ◽  
Dna Binding Proteins ◽  
Heat Shock Factor ◽  
Heat Shock Factor 1 ◽  
Circadian Transcription
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