Immunocytochemical Localization of Prunasin Hydrolase and Mandelonitrile Lyase in Stems and Leaves of Prunus serotina

Electron microscopic (EM) immunocytochemistry localization of the neuron specific protein p65 could show which organelles contain this antigen. Antibodies (Ab) labeled with horseradish peroxidase (HRP) followed by chromogen development show a broad diffuse label distribution within cells and restricting identification of organelles. Particulate label (e.g. 10 nm colloidal gold) is highly desirable but not practical because penetration into cells requires destroying the plasma membrane. We report pre-embedding immunocytochemistry with a particulate marker, 1 nm gold, that will pass through membranes treated with saponin, a mild detergent.Cell cultures of the rat cerebellum were fixed in buffered 4% paraformaldehyde and 0.1% glutaraldehyde (Glut.). The buffer for all incubations and rinses was phosphate buffered saline with: 1% calf serum, 0.2% saponin, 0.1% gelatin, 50 mM glycine 1 mg/ml bovine serum albumin, and (not in the HRP labeled cultures) 0.02% sodium azide. The monoclonal #48 to p65 was used with three label systems: HRP, 1 nm avidin gold with IntenSE M development, and 1 nm avidin gold with Danscher development.

Download Full-text

Immunocytochemical localization of desmin and vimentin in chick embryonic myocardial cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100159783 ◽

1990 ◽

Vol 48 (3) ◽

pp. 448-449

Author(s):

Yukiko Sugi

Keyword(s):

Sodium Methoxide ◽

Intermediate Filament Protein ◽

Chick Embryos ◽

Immunocytochemical Localization ◽

Myocardial Cells ◽

Immunofluorescence Study ◽

Immunofluorescent Localization ◽

Rabbit Igg ◽

Semithin Sections ◽

Filament Protein

In cultured skeletal muscle cells of chick, one intermediate filament protein, vimentin, is primarily formed and then synthesis of desmin follows. Coexistence of vimentin and desmin has been immunocytochemically confirmed in chick embryonic skeletal musclecells. Immunofluorescent localization of vimentin and desmin has been described in developing myocardial cells of hamster. However, initial localization of desmin and vimentin in early embryonic heart has not been reported in detail. By quick-freeze deep-etch method a loose network of intermediate filaments was revealed to exist surrounding myofibrils. In this report, immunocytochemical localization of desmin and vimentin is visualized in early stages of chick embryonic my ocardium.Chick embryos, Hamburger-Hamilton (H-H) stage 8 to hatch, and 1 day old postnatal chicks were used in this study. For immunofluorescence study, each embryo was fixed with 4% paraformaldehyde and embedded in Epon 812. De-epoxinized with sodium methoxide, semithin sections were stained with primary antibodies (rabbit anti-desmin antibody and anti-vimentin antibody)and secondary antibody (RITC conjugated goat-anti rabbit IgG).

Download Full-text

Pancreastatin: light and electron microscopical immunocytochemical localization of a novel peptide in porcine tissues

Acta Endocrinologica ◽

10.1530/acta.0.120s013 ◽

1989 ◽

Vol 120 (3_Suppl) ◽

pp. S13-S14

Author(s):

R. LAMBERTS ◽

W. SCHMIDT ◽

W. CREUTZFELD

Keyword(s):

Immunocytochemical Localization ◽

Electron Microscopical ◽

Porcine Tissues

Download Full-text

TISSUE DISTRIBUTION OF TRANSCRIPTS INVOLVED IN BIOSYNTHESIS OF BENZYLISOQUINOLINE ALKALOID IN MATURE PLANTS OF Argemone mexicana L. (Papaveraceae)

Revista Fitotecnia Mexicana ◽

10.35196/rfm.2018.1.13-21 ◽

2018 ◽

Vol 41 (1) ◽

pp. 13-21 ◽

Cited By ~ 1

Author(s):

Felipe Vázquez-Flota ◽

Jorge Rubio-Piña ◽

Jorge Xool-Tamayo ◽

Mariela Vergara-Olivares ◽

Yahaira Tamayo-Ordoñez ◽

...

Keyword(s):

Tissue Distribution ◽

Transport Mechanism ◽

Gene Products ◽

Argemone Mexicana ◽

Benzylisoquinoline Alkaloid ◽

Stems And Leaves ◽

Specific Reactions

The distribution of berberine and sanguinarine was analyzed in roots, stems and leaves of mature Argemone mexicana plants, along with that of transcripts corresponding to selected genes involved in both early biosynthetic reactions, which are common to both alkaloids, and in the late specific reactions conducting to the formation of each of them. Roots were the main sites of alkaloid accumulation, though they showed the lowest accumulation of the analyzed transcripts. Results are discussed in terms of the operation of a possible transport mechanism of alkaloids between the aerial tissues and the roots, or the occurrence of different biosynthetic alternative reactions in both parts, aerial and underground tissues, involving different gene products, yet with similar catalytic capacities.

Download Full-text