scholarly journals Purification and Characterization of Pea Seedling Amine Oxidase for Crystallization Studies

1994 ◽  
Vol 106 (3) ◽  
pp. 1205-1211 ◽  
Author(s):  
M. A. McGuirl ◽  
C. D. McCahon ◽  
K. A. McKeown ◽  
D. M. Dooley
Keyword(s):  
Amine Oxidase ◽  
Purification And Characterization ◽  
Pea Seedling

Purification and Characterization of Amine Oxidase from Soybean Seedlings

1993 ◽  
Vol 307 (1) ◽  
pp. 35-39 ◽  
Author(s):  
F. Vianello ◽  
M.L. Dipaolo ◽  
R. Stevanato ◽  
R. Gasparini ◽  
A. Rigo
Keyword(s):  
Amine Oxidase ◽  
Purification And Characterization ◽  
Soybean Seedlings

Purification and Characterization of Amine Oxidase from Pea Seedlings

1999 ◽  
Vol 15 (2) ◽  
pp. 196-201 ◽  
Author(s):  
Fabio Vianello ◽  
Armond Malek-Mirzayans ◽  
Maria Luisa Di Paolo ◽  
Roberto Stevanato ◽  
Adelio Rigo
Keyword(s):  
Amine Oxidase ◽  
Purification And Characterization ◽  
Pea Seedlings

Purification and characterization of phosphoglucomutase from peas

1994 ◽  
Vol 92 (3) ◽  
pp. 479-486 ◽  
Author(s):  
Cynthia M. Galloway ◽  
W. Mack Dugger
Keyword(s):  
Purification And Characterization

Purification and Characterization of Tissue-Type Plasminogen Activator in Maxillary Mucosa with Chronic Inflammation

1985 ◽  
Vol 54 (02) ◽  
pp. 485-489 ◽  
Author(s):  
Yukiyoshi Hamaguchi ◽  
Masuichi Ohi ◽  
Yasuo Sakakura ◽  
Yasuro Miyoshi
Keyword(s):  
Plasminogen Activator ◽  
Chronic Inflammation ◽  
Gel Filtration ◽  
Potassium Acetate ◽  
Tissue Type ◽  
Purification And Characterization ◽  
Tissue Type Plasminogen Activator ◽  
Urokinase Inhibitor ◽  
Type Plasminogen Activator

SummaryTissue-type plasminogen activator (TPA) was purified from maxillary mucosa with chronic inflammation and compared with urokinase. Purification procedure consisted of the extraction from delipidated mucosa with 0.3M potassium acetate buffer (pH 4.2), 66% saturation of ammonium sulfate, zinc chelate-Sepharose, concanavalin A-Sepharose and Sephadex G-100 gel filtration chromatographies.The molecular weight of the TPA was approximately 58,000 ± 3,000. Its activity was enhanced in the presence of fibrin and was quenched by placental urokinase inhibitor, but not quenched by anti-urokinase antibody. The TPA made no precipitin line against anti-urokinase antibody, while urokinase did.All these findings indicate that the TPA in maxillary mucosa with chronic inflammation is immunologically dissimilar to urokinase and in its affinity for fibrin.

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