scholarly journals Leaf Developmental Age Controls Expression of Genes Encoding Enzymes of Chlorophyll and Heme Biosynthesis in Pea (Pisum sativum L.)

1994 ◽  
Vol 106 (2) ◽  
pp. 537-546 ◽  
Author(s):  
Z. H. He ◽  
J. Li ◽  
C. Sundqvist ◽  
M. P. Timko
PLoS ONE ◽  
2015 ◽  
Vol 10 (3) ◽  
pp. e0119835 ◽  
Author(s):  
Vera S. Bogdanova ◽  
Olga O. Zaytseva ◽  
Anatoliy V. Mglinets ◽  
Natalia V. Shatskaya ◽  
Oleg E. Kosterin ◽  
...  

1988 ◽  
Vol 250 (1) ◽  
pp. 15-24 ◽  
Author(s):  
J A Gatehouse ◽  
D Bown ◽  
J Gilroy ◽  
M Levasseur ◽  
J Castleton ◽  
...  

A genomic clone from pea (Pisum sativum L.) contains all of one gene encoding a ‘minor’ (B-type) legumin polypeptide, and most of a second very similar gene. The two genes, designated LegJ and LegK, are arranged in tandem, separated by approx. 6 kb. A complete sequence of gene LegJ and its flanking sequences is given, with as much of the sequence of gene LegK as is present on the genomic clone. Hybridization of 3′ flanking sequence probes to seed mRNA, and sequence comparisons with cDNA species, suggested that gene LegJ, and probably gene LegK, was expressed. The partial amino acid sequences of ‘minor’ legumin α- and beta-polypeptides were used to confirm the identity of these genes. The transciption start in gene LegJ was mapped. The 5′ flanking sequence of gene LegJ contains a sequence conserved in legumin genes from pea and other species, which is likely to have functional significance in control of gene expression. Sequence comparisons with legumin genes and cDNA species from Vicia faba and soya bean show that separation of legumin genes into A- and B-type subfamilies occurred before separation of the Viciae and Glycinae tribes.


2021 ◽  
Vol 9 (12) ◽  
pp. 2458
Author(s):  
Alexey M. Afonin ◽  
Emma S. Gribchenko ◽  
Evgeny A. Zorin ◽  
Anton S. Sulima ◽  
Vladimir A. Zhukov

Rhizobium leguminosarum (Rl) is a common name for several genospecies of rhizobia able to form nitrogen-fixing nodules on the roots of pea (Pisum sativum L.) while undergoing terminal differentiation into a symbiotic form called bacteroids. In this work, we used Oxford Nanopore sequencing to analyze the genome methylation states of the free-living and differentiated forms of the Rl strain RCAM1026. The complete genome was assembled; no significant genome rearrangements between the cell forms were observed, but the relative abundances of replicons were different. GANTC, GGCGCC, and GATC methylated motifs were found in the genome, along with genes encoding methyltransferases with matching predicted target motifs. The GGCGCC motif was completely methylated in both states, with two restriction–modification clusters on different replicons enforcing this specific pattern of methylation. Methylation patterns for the GANTC and GATC motifs differed significantly depending on the cell state, which indicates their possible connection to the regulation of symbiotic differentiation. Further investigation into the differences of methylation patterns in the bacterial genomes coupled with gene expression analysis is needed to elucidate the function of bacterial epigenetic regulation in nitrogen-fixing symbiosis.


2020 ◽  
Vol 47 (5) ◽  
pp. 409 ◽  
Author(s):  
Galina Smolikova ◽  
Olga Shiroglazova ◽  
Galina Vinogradova ◽  
Irina Leppyanen ◽  
Ekaterina Dinastiya ◽  
...  

Developing seeds of some higher plants are photosynthetically active and contain chlorophylls (Chl), which are typically destroyed at the late stages of seed maturation. However, in some crop plant cultivars, degradation of embryonic Chl remains incomplete, and mature seeds preserve green colour, as it is known for green-seeded cultivars of pea (Pisum sativum L.). The residual Chl compromise seed quality and represent a severe challenge for farmers. Hence, comprehensive understanding of the molecular mechanisms, underlying incomplete Chl degradation is required for maintaining sustainable agriculture. Therefore, here we address dynamics of plastid conversion and photochemical activity alterations, accompanying degradation of Chl in embryos of yellow- and green-seeded cultivars Frisson and Rondo respectively. The yellow-seeded cultivar demonstrated higher rate of Chl degradation at later maturation stage, accompanied with termination of photochemical activity, seed dehydration and conversion of green plastids into amyloplasts. In agreement with this, expression of genes encoding enzymes of Chl degradation was lower in the green seeded cultivar, with the major differences in the levels of Chl b reductase (NYC1) and pheophytinase (PPH) transcripts. Thus, the difference between yellow and green seeds can be attributed to incomplete Chl degradation in the latter at the end of maturation period.


2018 ◽  
Vol 34 (2) ◽  
pp. 229-235 ◽  
Author(s):  
Prachi Garg ◽  
◽  
A. Hemantaranjan ◽  
Jyostnarani Pradhan ◽  
◽  
...  

2015 ◽  
Vol 50 (3) ◽  
pp. 353-360
Author(s):  
М.А. ВИШНЯКОВА ◽  
◽  
Е.В. СЕМЕНОВА ◽  
И.А. КОСАРЕВА ◽  
Н.Д. КРАВЧУК ◽  
...  

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