scholarly journals Environment or Development? Lifetime Net CO2 Exchange and Control of the Expression of Crassulacean Acid Metabolism in Mesembryanthemum crystallinum

2006 ◽  
Vol 143 (1) ◽  
pp. 98-107 ◽  
Author(s):  
Klaus Winter ◽  
Joseph A.M. Holtum
Keyword(s):  
Crassulacean Acid Metabolism ◽  
Acid Metabolism ◽  
Co2 Exchange ◽  
Mesembryanthemum Crystallinum ◽  
And Control

scholarly journals Transcriptional profiles of organellar metabolite transporters during induction of crassulacean acid metabolism in Mesembryanthemum crystallinum

2005 ◽  
Vol 32 (5) ◽  
pp. 451 ◽  
Author(s):  
Shin Kore-eda ◽  
Chiyuki Noake ◽  
Masahisa Ohishi ◽  
Jun-ichi Ohnishi ◽  
John C. Cushman
Keyword(s):  
Crassulacean Acid Metabolism ◽  
Acid Metabolism ◽  
Expression Patterns ◽  
Transcript Abundance ◽  
Transport Processes ◽  
Mesembryanthemum Crystallinum ◽  
Water Deficit Stress ◽  
Diurnal Changes ◽  
Metabolite Transport ◽  
Leaf Tissues

Metabolite transport across multiple organellar compartments is essential for the operation of crassulacean acid metabolism (CAM). To investigate potential circadian regulation of inter-organellar metabolite transport processes, we have identified eight full-length cDNAs encoding an organellar triose phosphate / Pi translocator (McTPT1), a phosphoenolpyruvate / Pi translocator (McPPT1), two glucose-6-phosphate / Pi translocators (McGPT1, 2), two plastidic Pi translocator-like proteins (McPTL1, 2), two adenylate transporters (McANT1, 2), a dicarboxylate transporter (McDCT2), and a partial cDNA encoding a second dicarboxylate transporter (McDCT1) in the model CAM plant, Mesembryanthemum crystallinum L. We next investigated day / night changes in steady-state transcript abundance of each of these transporters in plants performing either C3 photosynthesis or CAM induced by salinity or water-deficit stress. We observed that the expression of both isogenes of the glucose-6-phosphate / Pi translocator (McGPT1, 2) was enhanced by CAM induction, with McGPT2 transcripts exhibiting much more pronounced diurnal changes in transcript abundance than McGPT1. Transcripts for McTPT1, McPPT1, and McDCT1 also exhibited more pronounced diurnal changes in abundance in the CAM mode relative to the C3 mode. McGPT2 and McDCT1 transcripts exhibited sustained oscillations for at least 3 d under constant light and temperature conditions suggesting their expression is under circadian clock control. McTPT1 and McGPT2 transcripts were preferentially expressed in leaf tissues in either C3 or CAM modes. The leaf-specific and / or circadian controlled gene expression patterns are consistent with McTPT1, McGPT2 and McDCT1 playing CAM-specific metabolite transport roles.

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