scholarly journals Identification of Genes with Potential Roles in Apple Fruit Development and Biochemistry through Large-Scale Statistical Analysis of Expressed Sequence Tags

2006 ◽  
Vol 141 (3) ◽  
pp. 811-824 ◽  
Author(s):  
Sunchung Park ◽  
Nobuko Sugimoto ◽  
Matthew D. Larson ◽  
Randy Beaudry ◽  
Steven van Nocker
Keyword(s):  
Statistical Analysis ◽  
Fruit Development ◽  
Expressed Sequence Tags ◽  
Large Scale ◽  
Apple Fruit ◽  
Expressed Sequence

scholarly journals Large-scale identification of odorant-binding proteins and chemosensory proteins from expressed sequence tags in insects

BMC Genomics ◽  
2009 ◽  
Vol 10 (1) ◽  
pp. 632 ◽  
Author(s):  
Ya-Long Xu ◽  
Peng He ◽  
Lan Zhang ◽  
Shao-Qing Fang ◽  
Shuang-Lin Dong ◽  
...  
Keyword(s):  
Expressed Sequence Tags ◽  
Large Scale ◽  
Binding Proteins ◽  
Odorant Binding Proteins ◽  
Chemosensory Proteins ◽  
Odorant Binding ◽  
Expressed Sequence

scholarly journals Development of Microsatellite Markers Derived from Expressed Sequence Tags of Polyporales for Genetic Diversity Analysis of EndangeredPolyporus umbellatus

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Yuejin Zhang ◽  
Yuanyuan Chen ◽  
Ruihong Wang ◽  
Ailin Zeng ◽  
Michael K. Deyholos ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Expressed Sequence Tags ◽  
Large Scale ◽  
Gene Diversity ◽  
High Genetic Diversity ◽  
Information Index ◽  
Polyporus Umbellatus ◽  
Ssr Primers ◽  
Expressed Sequence

A large scale of EST sequences of Polyporales was screened in this investigation in order to identify EST-SSR markers for various applications. The distribution of EST sequences and SSRs in five families of Polyporales was analyzed, respectively. Mononucleotide was the most abundant type, followed by trinucleotide. Among five families, Ganodermataceae occupied the most SSR markers, followed by Coriolaceae. Functional prediction of SSR marker-containing EST sequences inGanoderma lucidumobtained three main groups, namely, cellular component, biological process, and molecular function. Thirty EST-SSR primers were designed to evaluate the genetic diversity of 13 naturalPolyporus umbellatusaccessions. Twenty one EST-SSRs were polymorphic with average PIC value of 0.33 and transferability rate of 71%. These 13P.umbellatusaccessions showed relatively high genetic diversity. The expected heterozygosity, Nei’s gene diversity, and Shannon information index were 0.41, 0.39, and 0.57, respectively. Both UPGMA dendrogram and principal coordinate analysis (PCA) showed the same cluster result that divided the 13 accessions into three or four groups.

scholarly journals Large-Scale Generation and Analysis of Expressed Sequence Tags from Porcine Ovary1

2004 ◽  
Vol 71 (6) ◽  
pp. 1991-2002 ◽  
Author(s):  
Honglin Jiang ◽  
Kristin M. Whitworth ◽  
Nathan J. Bivens ◽  
James E. Ries ◽  
Rami J. Woods ◽  
...  
Keyword(s):  
Expressed Sequence Tags ◽  
Large Scale ◽  
Expressed Sequence

Development and use of EST-SSR markers for assessing genetic diversity in the brown planthopper (Nilaparvata lugensStål)

2011 ◽  
Vol 102 (1) ◽  
pp. 113-122 ◽  
Author(s):  
S. Jing ◽  
B. Liu ◽  
L. Peng ◽  
X. Peng ◽  
L. Zhu ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Expressed Sequence Tags ◽  
Large Scale ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Rice Varieties ◽  
Genetics And Genomics ◽  
Expressed Sequence ◽  
Simple Sequence

AbstractTo assess genetic diversity in populations of the brown planthopper (Nilaparvata lugensStål) (Homoptera: Delphacidae), we have developed and applied microsatellite, or simple sequence repeat (SSR), markers from expressed sequence tags (ESTs). We found that the brown planthopper clusters of ESTs were rich in SSRs with unique frequencies and distributions of SSR motifs. Three hundred and fifty-one EST-SSR markers were developed and yielded clear bands from samples of four brown planthopper populations. High cross-species transferability of these markers was detected in the closely related planthopperN. muiri. The newly developed EST-SSR markers provided sufficient resolution to distinguish within and among biotypes. Analyses based on SSR data revealed host resistance-based genetic differentiation among different brown planthopper populations; the genetic diversity of populations feeding on susceptible rice varieties was lower than that of populations feeding on resistant rice varieties. This is the first large-scale development of brown planthopper SSR markers, which will be useful for future molecular genetics and genomics studies of this serious agricultural pest.

scholarly journals Construction of a full-length cDNA library from young spikelets of hexaploid wheat and its characterization by large-scale sequencing of expressed sequence tags

2004 ◽  
Vol 79 (4) ◽  
pp. 227-232 ◽  
Author(s):  
Yasunari Ogihara ◽  
Keiichi Mochida ◽  
Kanako Kawaura ◽  
Koji Murai ◽  
Motoaki Seki ◽  
...  
Keyword(s):  
Cdna Library ◽  
Expressed Sequence Tags ◽  
Hexaploid Wheat ◽  
Large Scale ◽  
Full Length ◽  
Full Length Cdna ◽  
Expressed Sequence
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