scholarly journals Carbonic Anhydrase and Its Influence on Carbon Isotope Discrimination during C4 Photosynthesis. Insights from Antisense RNA in Flaveria bidentis

2006 ◽  
Vol 141 (1) ◽  
pp. 232-242 ◽  
Author(s):  
Asaph B. Cousins ◽  
Murray R. Badger ◽  
Susanne von Caemmerer
Keyword(s):  
Carbonic Anhydrase ◽  
Carbon Isotope ◽  
Antisense Rna ◽  
Carbon Isotope Discrimination ◽  
C4 Photosynthesis ◽  
Isotope Discrimination ◽  
Flaveria Bidentis

Carbon Isotope Discrimination during C4 Photosynthesis: Insights from Transgenic Plants

1997 ◽  
Vol 24 (4) ◽  
pp. 487 ◽  
Author(s):  
Susanne von Caemmerer ◽  
Martha Ludwig ◽  
Anthony Millgate ◽  
Graham D. Farquhar ◽  
Dean Price ◽  
...  
Keyword(s):  
Carbon Isotope ◽  
Carbon Isotope Discrimination ◽  
Co2 Assimilation ◽  
Small Subunit ◽  
Bundle Sheath ◽  
Cauliflower Mosaic Virus ◽  
35S Promoter ◽  
Coding Region ◽  
Isotope Discrimination ◽  
Flaveria Bidentis

We have measured the discrimination against 13C during CO2 assimilation in Flaveria bidentis wild type plants and in transgenic Flaveria bidentis plants transformed (1) with an antisense RNA construct targeted to the nuclear encoded gene for the small subunit of Rubisco—these plants had reduced amounts of Rubisco, decreased CO2 assimilation rates and increased carbon isotope discrimination, which was also evident in the carbon isotope discrimination of leaf dry matter; and (2) transformed with the mature coding region of carbonic anhydrase, CA, from tobacco (Nicotiana tabacum) in the sense direction under the control of the cauliflower mosaic virus 35S promoter—these plants had slightly increased CA activity in the mesophyll as well as a 2–4-fold increase in CA activity in the bundle-sheath cells. The introduction of tobacco CA manifested itself by a reduction in CO2 assimilation rate and an increase in carbon isotope discrimination. We suggest that the increased carbon isotope discrimination is a result of increased bicarbonate leakage out of the bundle sheath.

scholarly journals Carbon isotope discrimination as a tool to explore C4 photosynthesis

2014 ◽  
Vol 65 (13) ◽  
pp. 3459-3470 ◽  
Author(s):  
Susanne von Caemmerer ◽  
Oula Ghannoum ◽  
Jasper J. L. Pengelly ◽  
Asaph B. Cousins
Keyword(s):  
Carbon Isotope ◽  
Carbon Isotope Discrimination ◽  
C4 Photosynthesis ◽  
Isotope Discrimination
Sign in / Sign up

Export Citation Format

Share Document