scholarly journals Cell-Autonomous Cytokinin-Independent Growth of Tobacco Cells Transformed by Agrobacterium tumefaciens Strains Lacking the Cytokinin Biosynthesis Gene

1994 ◽  
Vol 105 (3) ◽  
pp. 989-998 ◽  
Author(s):  
R. C. Black ◽  
A. N. Binns ◽  
C. F. Chang ◽  
D. G. Lynn
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Cytokinin Biosynthesis ◽  
Tobacco Cells ◽  
Biosynthesis Gene

scholarly journals Regulated Expression of a Cytokinin Biosynthesis Gene IPT Delays Leaf Senescence and Improves Yield under Rainfed and Irrigated Conditions in Canola (Brassica napus L.)

PLoS ONE ◽  
2015 ◽  
Vol 10 (1) ◽  
pp. e0116349 ◽  
Author(s):  
Surya Kant ◽  
David Burch ◽  
Pieter Badenhorst ◽  
Rajasekaran Palanisamy ◽  
John Mason ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Leaf Senescence ◽  
Brassica Napus L ◽  
Cytokinin Biosynthesis ◽  
Regulated Expression ◽  
Biosynthesis Gene

Two Agrobacterium tumefaciens genes for cytokinin biosynthesis: Ti plasmid-coded isopentenyltransferases adapted for function in prokaryotic or eukaryotic cells

1987 ◽  
Vol 210 (1) ◽  
pp. 156-164 ◽  
Author(s):  
Wolfgang Heinemeyer ◽  
Ilka Buchmann ◽  
Dave W. Tonge ◽  
John D. Windass ◽  
Juliane Alt-Moerbe ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Ti Plasmid ◽  
Eukaryotic Cells ◽  
Cytokinin Biosynthesis

scholarly journals EXPRESSION AND PURIFICATION OF THE IPT GENE

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 580g-581
Author(s):  
Sandra L. Barbour ◽  
D.A. Schaff ◽  
A.C. Smigocki ◽  
J.J. Frett
Keyword(s):  
Agrobacterium Tumefaciens ◽  
New England ◽  
Cloning And Expression ◽  
Isopentenyl Transferase ◽  
Cytokinin Biosynthesis ◽  
E Coli ◽  
Shoot Production ◽  
Cytokinin Synthesis ◽  
Polymerase Chain

The ipt gene of Agrobacterium tumefaciens T-DNA encodes for isopentenyl transferase, which is an enzyme active in cytokinin biosynthesis. While it is known that cytokinins are associated with in vitro promotion of cell division and stimulation of shoot production, little is known about their mode of action. As the first step in localizing cytokinin synthesis, we present a cloning and expression strategy for the ipt gene. The source of the ipt gene was Agrobacterium tumefaciens octopine Ti plasmid 15955. The ipt gene was amplified by the polymerase chain reaction (PCR) and cloned into pMal-c2 (New England Biolabs, Beverly, MA). This construct was transformed into E. coli and the ipt gene was expressed as a fusion protein. The protein was purified by affinity chromatography to serve as an antigen for polyclonal antibody production. These antibodies will be used to localize isopentenyl transferase in plant tissue.

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