scholarly journals Isolation of cDNA Clones for Genes That Are Expressed during Leaf Senescence in Brassica napus (Identification of a Gene Encoding a Senescence-Specific Metallothionein-Like Protein)

1994 ◽  
Vol 105 (3) ◽  
pp. 839-846 ◽  
Author(s):  
V. Buchanan-Wollaston
Keyword(s):  
Brassica Napus ◽  
Leaf Senescence ◽  
Cdna Clones ◽  
Gene Encoding

scholarly journals Dehiscence-related expression of an Arabidopsis thaliana gene encoding a polygalacturonase in transgenic plants of Brassica napus

1999 ◽  
Vol 22 (2) ◽  
pp. 159-167 ◽  
Author(s):  
E. S. JENKINS ◽  
W. PAUL ◽  
M. CRAZE ◽  
C. A. WHITELAW ◽  
A. WEIGAND ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Brassica Napus ◽  
Transgenic Plants ◽  
Gene Encoding

scholarly journals Structure, expression and phylogenetic analysis of the gene encoding actin I in Pneumocystis carinii.

Genetics ◽  
1994 ◽  
Vol 137 (3) ◽  
pp. 743-750 ◽  
Author(s):  
L D Fletcher ◽  
J M McDowell ◽  
R R Tidwell ◽  
R B Meagher ◽  
C C Dykstra
Keyword(s):  
Phylogenetic Analysis ◽  
Essential Gene ◽  
Pneumocystis Carinii ◽  
Comparative Sequence Analysis ◽  
Cdna Clones ◽  
Actin Gene ◽  
Gene Encoding ◽  
Actin Protein ◽  
Relationship Of ◽  
High Degree

Abstract Actin is a major component of the cytoskeleton and one of the most abundant proteins found in eukaryotic cells. Comparative sequence analysis shows that this essential gene has been highly conserved throughout eukaryotic evolution making it useful for phylogenetic analysis. Complete cDNA clones for the actin-encoding gene were isolated and characterized from Pneumocystis carinii purified from immunosuppressed rat lungs. The nucleotide sequence encodes a protein of 376 amino acids. The predicted actin protein of P. carinii shares a high degree of conservation to other known actins. Only one major actin gene was found in P. carinii. The P. carinii actin sequence was compared with 30 other actin sequences. Gene phylogenies constructed using both neighbor-joining and protein parsimony methods places the P. carinii actin sequence closest to the majority of the fungi. Since the phylogenetic relationship of P. carinii to fungi and protists has been questioned, these data on the actin gene phylogeny support the grouping of P. carinii with the fungi.

scholarly journals Bean polygalacturonase-inhibiting protein expressed in transgenic Brassica napus inhibits polygalacturonase from its fungal pathogen Rhizoctonia solani

2012 ◽  
Vol 48 (No. 1) ◽  
pp. 1-9 ◽  
Author(s):  
A.B. Akhgari ◽  
M. Motallebi ◽  
M.R. Zamani
Keyword(s):  
Brassica Napus ◽  
Rhizoctonia Solani ◽  
Pathogenic Fungi ◽  
Gene Encoding ◽  
Transgenic Canola ◽  
Antifungal Potential ◽  
Functional Inhibition ◽  
Commercially Important ◽  
Polygalacturonase Inhibiting Protein ◽  
Polymerase Chain

Polygalacturonase-inhibiting proteins (PGIPs) selectively inhibit polygalacturonases (PGs) secreted by invading plant pathogenic fungi. The objective of present research was to clone and introduce the pgip2 gene from bean (Phaseolus vulgaris) cv. Goli, with antifungal potential, into the commercially important canola (Brassica napus, R line Hyola 308) via Agrobacterium tumefaciens mediated transformation. Here we used a transgenic overexpression approach in order to investigate the inhibitory activity of the PGIP on the PG from Rhizoctonia solani, the causal agent of damping off and root rot of canola. PGIP expression was determined in the functional inhibition assays against fungal PGs. Crude protein extracts prepared from transgenic canola leaves were found to inhibit the R. solani PG from 29% to 37% as compared to untransformed plants. The putative transgenic canola lines harbouring the pgip2 gene encoding polygalacturonase-inhibiting proteins were identified by polymerase chain reaction and Southern blot analysis.  

scholarly journals Regulated Expression of a Cytokinin Biosynthesis Gene IPT Delays Leaf Senescence and Improves Yield under Rainfed and Irrigated Conditions in Canola (Brassica napus L.)

PLoS ONE ◽  
2015 ◽  
Vol 10 (1) ◽  
pp. e0116349 ◽  
Author(s):  
Surya Kant ◽  
David Burch ◽  
Pieter Badenhorst ◽  
Rajasekaran Palanisamy ◽  
John Mason ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Leaf Senescence ◽  
Brassica Napus L ◽  
Cytokinin Biosynthesis ◽  
Regulated Expression ◽  
Biosynthesis Gene

scholarly journals Characterization of the rat salivary-gland B1-immunoreactive proteins

1998 ◽  
Vol 330 (1) ◽  
pp. 437-444 ◽  
Author(s):  
Lily MIRELS ◽  
J. Abigail MIRANDA ◽  
D. William BALL
Keyword(s):  
Submandibular Gland ◽  
Protein A ◽  
Secretory Protein ◽  
Cdna Clones ◽  
Parotid Glands ◽  
Gene Encoding ◽  
Adult Rat ◽  
Related Clone ◽  
Secretory Products

The B1-immunoreactive proteins (B1-IPs) are major secretory products of rat submandibular gland acinar-cell progenitors, and are also produced by neonatal and adult rat sublingual and parotid glands. In order to characterize the B1-IPs, we have previously isolated cDNA clones encoding rat parotid secretory protein (PSP; the predominant parotid B1-IP) and the related clone ZZ3, which is developmentally regulated in the neonatal submandibular gland. The remainder of the B1-IPs were uncharacterized. This report demonstrates that all of the B1-IPs are derived from the PSP and ZZ3 transcripts. Molecular cloning and Western-blot analyses using PSP- and ZZ3-specific antisera show that, of the B1-IPs, only PSP and neonatal submandibular gland protein A (SMGA) are products of the Psp gene. This finding corrects our previous assertion that SMGA is derived from ZZ3. Neonatal submandibular gland proteins B1 and B2, as well as apparent Mr 26000-28000 and Mr 18000-20000 forms in submandibular, sublingual and parotid glands, are derived from the gene encoding ZZ3 by differential N-glycosylation and by proteolytic cleavage. The apparent Mr 18000-20000 proteolytic products are significant in secretion product collected in vitro, but rare in gland homogenate and submandibular/sublingual saliva. The gene encoding ZZ3 has been named Smgb. Psp and Smgb are regulated similarly in the developing submandibular gland, but differently in the sublingual and parotid glands. The expression pattern of Psp is conserved between rat and mouse. However, no evidence for proteins derived from an Smgb-like gene was observed in neonatal mouse submandibular or sublingual glands.

scholarly journals Molecular Characterization of a Senescence-Associated Gene Encoding Cysteine Proteinase and its Gene Expression during Leaf Senescence in Sweet Potato

2002 ◽  
Vol 43 (9) ◽  
pp. 984-991 ◽  
Author(s):  
Guan-Hong Chen ◽  
Lin-Tzu Huang ◽  
Mee-Ngan Yap ◽  
Ruey-Hua Lee ◽  
Yih-Jong Huang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Molecular Characterization ◽  
Sweet Potato ◽  
Leaf Senescence ◽  
Cysteine Proteinase ◽  
Gene Encoding ◽  
Its Gene

scholarly journals Isolation and functional analysis of a cDNA for human Jagged2, a gene encoding a ligand for the Notch1 receptor.

1997 ◽  
Vol 17 (10) ◽  
pp. 6057-6067 ◽  
Author(s):  
B Luo ◽  
J C Aster ◽  
R P Hasserjian ◽  
F Kuo ◽  
J Sklar
Keyword(s):  
Feedback Control ◽  
Expressed Sequence Tag ◽  
Myogenic Differentiation ◽  
Notch Receptor ◽  
Full Length ◽  
C2c12 Cells ◽  
Cdna Clones ◽  
Gene Encoding ◽  
Full Length Cdna

Signaling through Notch receptors has been implicated in the control of cellular differentiation in animals ranging from nematodes to humans. Starting from a human expressed sequence tag-containing sequence resembling that of Serrate, the gene for a ligand of Drosophila melanogaster Notch, we assembled a full-length cDNA, now called human Jagged2, from overlapping cDNA clones. The full-length cDNA encodes a polypeptide having extensive sequence homology to Serrate (40.6% identity and 58.7% similarity) and even greater homology to several putative mammalian Notch ligands that have subsequently been described. When in situ hybridization was performed, expression of the murine Jagged2 homolog was found to be highest in fetal thymus, epidermis, foregut, dorsal root ganglia, and inner ear. In Northern blot analysis of RNA from tissues of 2-week-old mice, the 5.0-kb Jagged2 transcript was most abundant in heart, lung, thymus, skeletal muscle, brain, and testis. Immunohistochemistry revealed coexpression of Jagged2 and Notch1 within thymus and other fetal murine tissues, consistent with interaction of the two proteins in vivo. Coculture of fibroblasts expressing human Jagged2 with murine C2C12 myoblasts inhibited myogenic differentiation, accompanied by increased Notch1 and the appearance of a novel 115-kDa Notch1 fragment. Exposure of C2C12 cells to Jagged2 led to increased amounts of Notch mRNA as well as mRNAs for a second Notch receptor, Notch3, and a second Notch ligand, Jagged1. Constitutively active forms of Notchl in C2C12 cells also induced increased levels of the same set of mRNAs, suggesting positive feedback control of these genes initiated by binding of Jagged2 to Notch1. This feedback control may function in vivo to coordinate differentiation across certain groups of progenitor cells adopting identical cell fates.

scholarly journals Aktivitas ACCase mesokarp kelapa sawit dan kloning fragmen gen penyandi ACCase subunit biotin karboksilase ACCase activity of oil palm mesocarp and cloning of gene fragment encoding biotin carboxylase subunit of ACCase

2016 ◽  
Vol 74 (1) ◽  
Author(s):  
Asmini BUDIANI ◽  
Djoko SANTOSO ◽  
Hajrial ASWIDINNOOR ◽  
Antonius SUWANTO
Keyword(s):  
Arabidopsis Thaliana ◽  
Brassica Napus ◽  
Nicotiana Tabacum ◽  
Oil Palm ◽  
Annealing Temperature ◽  
Rt Pcr ◽  
Cdna Fragment ◽  
Gene Encoding ◽  
Conserved Region ◽  
Cloned Cdna

Summary Genetic engineering to produce high yielding oil palm might be done by over expressing gene encoding key enzyme for oil biosynthesis in the oil palm mesocarp, one of which is ACCase. The objective of this research was to analyze ACCase activity of mesocarp from several developmental stages of fruit and to clone conserved region cDNA of gene encoding biotin carboxylase subunit of ACCase (BC-htACCase) from oil palm mesocarp. Activity of ACCase was analyzed by HPLC. Amplification of cDNA was done by means of reverse transcriptase polymerase chain reaction (RT-PCR) using degenerate heterologous primer on several annealing temperature and MgCl2 concentration. The cDNA fragment of RT-PCR product was cloned, sequenced and analyzed to confirm that the cloned cDNA was conserved region of BC-htACCase. The result showed that ACCase activity increased from the 14 week to the 20 week-old fruit, and then decreased. Using heterologous degenerate primers, cDNA fragments of BC-htACCase conserved region (469 bp) can be specifically amplified at 60 oC annealing temperature with 2 mM MgCl2 concentration.The result of BlastX analysis showed that the sequence of cloned cDNA fragment was highly homologous with the conserved region of BC-htACCase from Glycine max, Arabidopsis thaliana, Nicotiana tabacum,  and Brassica napus with 243, 237, 236, 231 bit score, and E. value 2e-63, 1e-61, 2e-61 and 5e-60, respectively. Ringkasan Rekayasa genetika untuk menghasilkan bibit kelapa sawit berdaya hasil tinggi dapat ditempuh dengan meningkatkan ekspresi gen penyandi enzim kunci biosintesis minyak pada kelapa sawit, salah satunya adalah ACCase. Tujuan penelitian ini adalah menguji aktivitas ACCase mesokarp beberapa tahap perkem-bangan buah sawit dan mengklon fragmen cDNA daerah konservatif gen penyandi ACCase heteromerik subunit biotin karbok-silase (BC-htACCase) dari mesokarp buah sawit. Aktivitas ACCase dianalisis dengan HPLC. Amplifikasi cDNA dilakukan dengan teknik RT-PCR menggunakan primer degene-rate heterologus pada berbagai suhu penempelan dan konsentrasi MgCl2. Fragmen cDNA hasil RT-PCR diklon, disekuen dan dianalisis untuk mengkonfirmasi bahwa cDNA terklon adalah daerah konservatif BC-htACCase. Hasil penelitian menunjukkan bahwa aktivitas ACCase meningkat dari buah berumur 14 minggu hingga buah berumur  20 minggu, kemudian menurun kembali Dengan primer degenerate heterologus, fragmen cDNA daerah konservatif BC-htACCase  (469 pb) dapat diamplifikasi secara spesifik pada suhu penempelan 60 oC dan konsentrasi MgCl2 2 mM. Hasil analisis BlastX dari sekuen DNA fragmen terklon menunjuk-kan bahwa sekuen tersebut mempunyai homologi tinggi antara lain dengan gen penyandi BC-htACCase dari Glycine max, Arabidopsis thaliana, Nicotiana tabacum dan Brassica napus, masing-masing dengan skor 243, 237, 236, 231 bit, dan E. value 2e-63, 1e-61, 2e-61 dan 5e-60.

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