scholarly journals Virus-Induced Gene Silencing-Based Functional Characterization of Genes Associated with Powdery Mildew Resistance in Barley

2005 ◽  
Vol 138 (4) ◽  
pp. 2155-2164 ◽  
Author(s):  
Ingo Hein ◽  
Maria Barciszewska-Pacak ◽  
Katarina Hrubikova ◽  
Sandie Williamson ◽  
Malene Dinesen ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Gene Silencing ◽  
Powdery Mildew Resistance ◽  
Functional Characterization ◽  
Virus Induced Gene Silencing ◽  
Mildew Resistance

Characterization of Pm59, a novel powdery mildew resistance gene in Afghanistan wheat landrace PI 181356

2018 ◽  
Vol 131 (5) ◽  
pp. 1145-1152 ◽  
Author(s):  
Chengcheng Tan ◽  
Genqiao Li ◽  
Christina Cowger ◽  
Brett F. Carver ◽  
Xiangyang Xu
Keyword(s):  
Powdery Mildew ◽  
Resistance Gene ◽  
Powdery Mildew Resistance ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance ◽  
Wheat Landrace

Characterization of the Powdery Mildew Resistance Gene in Wheat Breeding Line KN0816 and its Evaluation in Marker-Assisted Selection

Plant Disease ◽  
2021 ◽  
Author(s):  
Wenrui Wang ◽  
Huagang He ◽  
Huiming Gao ◽  
Hongxing Xu ◽  
Wenyue Song ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Resistance Gene ◽  
Marker Assisted Selection ◽  
Powdery Mildew Resistance ◽  
Wheat Breeding ◽  
Agronomic Performance ◽  
Breeding Line ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance

Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a destructive disease seriously threatening yield and quality of common wheat (Triticum aestivum L., 2n=6x=42, AABBDD). Characterization of resistance genes against powdery mildew is useful in parental selection and for developing disease resistant cultivars. Chinese wheat breeding line KN0816 has superior agronomic performance and resistance to powdery mildew at all growth stages. Genetic analysis using populations of KN0816 crossed with different susceptible parents indicated that a single dominant gene, tentatively designated PmKN0816, conferred seedling resistance to different Bgt isolates. Using a bulked segregant analysis (BSA), PmKN0816 was mapped to the Pm6 interval on chromosome arm 2BL using polymorphic markers linked to the catalogued genes Pm6, Pm52, and Pm64, and flanked by markers CISSR02g-6 and CIT02g-2 both with genetic distances of 0.7 cM. Analysis of closely linked molecular markers indicated that the marker alleles of PmKN0816 differed from those of other powdery mildew resistance genes on 2BL, including Pm6, Pm33, Pm51, Pm64, and PmQ. Based on the genetic and physical locations and response pattern to different Bgt isolates, PmKN0816 is most likely a new powdery mildew resistance gene and confers effective resistance to all the 14 tested Bgt isolates. In view of the elite agronomic performance of KN0816 combined with the resistance, PmKN0816 is expected to become a valuable resistance gene in wheat breeding. To transfer PmKN0816 to different genetic backgrounds using marker-assisted selection (MAS), closely linked markers of PmKN0816 were evaluated and four of them (CIT02g-2, CISSR02g-6, CIT02g-10, and CIT02g-17) were confirmed to be applicable for MAS in different genetic backgrounds.

scholarly journals Phenotypic characterization of papaya genotypes to determine powdery mildew resistance

2017 ◽  
Vol 17 (3) ◽  
pp. 198-205
Author(s):  
Marcelo Vivas ◽  
Silvaldo Felipe da Silveira ◽  
Janieli Maganha Silva Vivas ◽  
Pedro Henrique Dias dos Santos ◽  
Beatriz Murizini Carvalho ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Powdery Mildew Resistance ◽  
Phenotypic Characterization ◽  
Mildew Resistance

scholarly journals Naturally Occurring Broad-Spectrum Powdery Mildew Resistance in a Central American Tomato Accession Is Caused by Loss of Mlo Function

2008 ◽  
Vol 21 (1) ◽  
pp. 30-39 ◽  
Author(s):  
Yuling Bai ◽  
Stefano Pavan ◽  
Zheng Zheng ◽  
Nana F. Zappel ◽  
Anja Reinstädler ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Broad Spectrum ◽  
Powdery Mildew Resistance ◽  
Candidate Gene Approach ◽  
Loss Of Function ◽  
Virus Induced Gene Silencing ◽  
Coding Region ◽  
Oidium Neolycopersici ◽  
Tomato Cultivar ◽  
Mildew Resistance

The resistant cherry tomato (Solanum lycopersicum var. cerasiforme) line LC-95, derived from an accession collected in Ecuador, harbors a natural allele (ol-2) that confers broad-spectrum and recessively inherited resistance to powdery mildew (Oidium neolycopersici). As both the genetic and phytopathological characteristics of ol-2–mediated resistance are reminiscent of powdery mildew immunity conferred by loss-of-function mlo alleles in barley and Arabidopsis, we initiated a candidate-gene approach to clone Ol-2. A tomato Mlo gene (SlMlo1) with high sequence-relatedness to barley Mlo and Arabidopsis AtMLO2 mapped to the chromosomal region harboring the Ol-2 locus. Complementation experiments using transgenic tomato lines as well as virus-induced gene silencing assays suggested that loss of SlMlo1 function is responsible for powdery mildew resistance conferred by ol-2. In progeny of a cross between a resistant line bearing ol-2 and the susceptible tomato cultivar Moneymaker, a 19-bp deletion disrupting the SlMlo1 coding region cosegregated with resistance. This polymorphism results in a frameshift and, thus, a truncated nonfunctional SlMlo1 protein. Our findings reveal the second example of a natural mlo mutant that possibly arose post-domestication, suggesting that natural mlo alleles might be evolutionarily short-lived due to fitness costs related to loss of mlo function.

Characterization of PmDGM conferring powdery mildew resistance in Chinese wheat landrace Duanganmang

Plant Disease ◽  
2021 ◽  
Author(s):  
Yanan Wu ◽  
Xiaoting Yu ◽  
Xu Zhang ◽  
Lijuan Yan ◽  
Li Gao ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Powdery Mildew Resistance ◽  
Single Nucleotide Polymorphism Array ◽  
Single Nucleotide ◽  
Mildew Resistance ◽  
Wheat Landrace ◽  
Yield And Quality ◽  
Genome Homology ◽  
Chinese Wheat

Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a devastating disease threatening yield and quality. Host resistance is considered the most effective and preferred means to control this disease. Wheat landrace Duanganmang (DGM) showed high resistance or near immunity to Bgt mixture from Henan province, China. DGM was crossed with highly susceptible Chinese wheat landrace Huixianhong (HXH) and cultivar Shimai 15 (SM15) to produce genetic populations. The resistance of DGM to Bgt isolate E09 was shown to be controlled by a single dominant Mendelian factor, tentatively designated PmDGM. Marker analysis and 55K SNP (single nucleotide polymorphism) array scanning showed that this gene was positioned in the Pm5 interval (2.4 cM or 1.61 Mb) flanked by Xhenu099 and Xmp1158 in the Chinese Spring reference genome. Homology-based cloning and sequence analysis demonstrated that DGM has the identical NLR gene (Pm5e) and RXL gene reported in Fuzhuang 30 (FZ30) conferring and modifying the powdery mildew resistance, respectively. However, based on the different reaction patterns to the Bgt isolate B15 between DGM and FZ30, we speculate that DGM may have two tightly linked genes that could not be separated in the current mapping population, one is PmDGM and the other is Pm5e. Hence, this study provides a valuable resistance resource for improvement of powdery mildew resistance.

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