scholarly journals The Involvement of Respiration in Free Radical Processes during Loss of Desiccation Tolerance in Germinating Zea mays L. (An Electron Paramagnetic Resonance Study)

1994 ◽  
Vol 104 (4) ◽  
pp. 1333-1339 ◽  
Author(s):  
O. Leprince ◽  
N. M. Atherton ◽  
R. Deltour ◽  
GAF. Hendry
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Zea Mays ◽  
Free Radical ◽  
Desiccation Tolerance ◽  
Zea Mays L ◽  
Electron Paramagnetic Resonance Study ◽  
Paramagnetic Resonance ◽  
Free Radical Processes ◽  
Radical Processes ◽  
Electron Paramagnetic

Free radical processes induced by desiccation in germinating maize. The relationship with respiration and loss of desiccation tolerance

1994 ◽  
Vol 102 ◽  
pp. 211-218 ◽  
Author(s):  
O. Leprince ◽  
G. A. F. Hendry ◽  
N. M. Atherton
Keyword(s):  
Free Radical ◽  
Desiccation Tolerance ◽  
Respiratory Enzymes ◽  
Stable Free Radical ◽  
Paramagnetic Resonance ◽  
Free Radical Processes ◽  
Transport Chain ◽  
Resonance Response ◽  
Radical Processes

SynopsisUsing germination of maize as a model, desiccation-induced free radical processes were studied with the object of understanding desiccation tolerance. Several significant elements of damage were observed in desiccated material associated with development of desiccation intolerance: increased lipid peroxidation, phospholipid de-esterification, build-up of a stable free radical, supression or repression of respiratory enzymes from complex I, II and IV. An EPR (electron paramagnetic resonance) response was also detected in isolated mitochondria following in vitro desiccation. The loss of desiccation tolerance appeared to be dependent on oxygen concentration. Two highly significant correlations were independently found between respiration rates and production of a stable free radical detected by EPR. These data suggest that respiration is an important factor in the loss of desiccation tolerance. We present a model suggesting that activated oxygen formation during desiccation originates in the disruption of the mitochondrial electron transport chain with increasing leakage to oxygen so generating irreversible and lethal peroxidative damage, leading to the development of desiccation intolerance.

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