scholarly journals Overlaps in the Transcriptional Profiles of Medicago truncatula Roots Inoculated with Two Different Glomus Fungi Provide Insights into the Genetic Program Activated during Arbuscular Mycorrhiza

2005 ◽  
Vol 137 (4) ◽  
pp. 1283-1301 ◽  
Author(s):  
Natalija Hohnjec ◽  
Martin F. Vieweg ◽  
Alfred Pühler ◽  
Anke Becker ◽  
Helge Küster
Keyword(s):  
Arbuscular Mycorrhiza ◽  
Medicago Truncatula ◽  
Genetic Program ◽  
Transcriptional Profiles

scholarly journals Differential Expression of Eight Chitinase Genes in Medicago truncatula Roots During Mycorrhiza Formation, Nodulation, and Pathogen Infection

2000 ◽  
Vol 13 (7) ◽  
pp. 763-777 ◽  
Author(s):  
Peter Salzer ◽  
Athos Bonanomi ◽  
Katinka Beyer ◽  
Regina Vögeli-Lange ◽  
Roger A. Aeschbacher ◽  
...  
Keyword(s):  
Arbuscular Mycorrhiza ◽  
Medicago Truncatula ◽  
Glomus Intraradices ◽  
Chitinase Gene ◽  
Class I ◽  
Specific Class ◽  
Class Iii ◽  
Phytophthora Megasperma ◽  
Class Iii Chitinase ◽  
Chitinase Genes

Expression of eight different chitinase genes, representing members of five chitinase classes, was studied in Medicago truncatula roots during formation of arbuscular mycorrhiza with Glomus intraradices, nodulation with Rhizobium meliloti, and pathogen attack by Phytophthora megasperma f. sp. medicaginis, Fusarium solani f. sp. phaseoli (compatible interactions with root rot symptoms), Ascochyta pisi (compatible, symptomless), and F. solani f. sp. pisi (incompatible, nonhost interaction). In the compatible plant-pathogen interactions, expression of class I, II, and IV chitinase genes was enhanced. The same genes were induced during nodulation. Transcripts of class I and II chitinase genes accumulated transiently during early stages of the interaction, and transcripts of the class IV chitinase gene accumulated in mature nodules. The pattern of chitinase gene expression in mycorrhizal roots was markedly different: Expression of class I, II, and IV chitinase genes was not enhanced, whereas expression of three class III chitinase genes, with almost no basal expression, was strongly induced. Two of these three (Mtchitinase III-2 and Mtchitinase III-3) were not induced at all in interactions with pathogens and rhizobia. Thus, the expression of two mycorrhiza-specific class III chitinase genes can be considered a hallmark for the establishment of arbuscular mycorrhiza in Medicago truncatula.

scholarly journals Arbuscular mycorrhiza in mini-mycorrhizotrons: first contact of Medicago truncatula roots with Glomus intraradices induces chalcone synthase

2001 ◽  
Vol 150 (3) ◽  
pp. 573-582 ◽  
Author(s):  
Athos Bonanomi ◽  
Jürg H. Oetiker ◽  
Richard Guggenheim ◽  
Thomas Boller ◽  
Andres Wiemken ◽  
...  
Keyword(s):  
Arbuscular Mycorrhiza ◽  
Medicago Truncatula ◽  
Glomus Intraradices ◽  
Chalcone Synthase ◽  
First Contact

Medicago truncatula gene responses specific to arbuscular mycorrhiza interactions with different species and genera of Glomeromycota

Mycorrhiza ◽  
2007 ◽  
Vol 17 (3) ◽  
pp. 223-234 ◽  
Author(s):  
M. Massoumou ◽  
D. van Tuinen ◽  
O. Chatagnier ◽  
C. Arnould ◽  
L. Brechenmacher ◽  
...  
Keyword(s):  
Arbuscular Mycorrhiza ◽  
Medicago Truncatula

scholarly journals Transcriptional Changes in Response to Arbuscular Mycorrhiza Development in the Model Plant Medicago truncatula

2003 ◽  
Vol 16 (4) ◽  
pp. 306-314 ◽  
Author(s):  
Anne Wulf ◽  
Katja Manthey ◽  
Jasmin Doll ◽  
Andreas M. Perlick ◽  
Burkhard Linke ◽  
...  
Keyword(s):  
Arbuscular Mycorrhiza ◽  
Medicago Truncatula ◽  
Expressed Sequence Tag ◽  
Gene Expression Pattern ◽  
Cdna Libraries ◽  
Suppressive Subtractive Hybridization ◽  
Specific Gene ◽  
Cdna Sequences ◽  
Wide Range ◽  
Transcriptional Changes

Significant changes in root morphology and physiology during arbuscular mycorrhiza (AM) development are likely to be controlled by specific gene expression pattern in the host plant. Until now, little was known about transcriptional changes which occur AM-exclusively; that is, they do not occur during other root-microbe associations, nor are they induced by improved phosphate nutrition. In order to identify such AM-exclusive gene inductions of Medicago truncatula, we used a pool of different RNA samples as subtractor population in a suppressive subtractive hybridization (SSH) experiment. This approach resulted in the identification of a number of new AM-regulated genes. None of these genes were expressed in nonmycorrhiza roots or leaves. Electronic data obtained by comparison of the cDNA sequences to expressed sequence tag (EST) sequences from a wide range of cDNA libraries in the M. truncatula EST database (Gene Index, MtGI) support the mycorrhiza specificity of the corresponding genes, because sequences in the MtGI that were found to match the identified SSH-cDNA sequences originated exclusively from AM cDNA libraries. The promoter of one of those genes, MtGst1, showing similarities to plant glutathione-S-transferase (GST) encoding genes, was cloned and used in reporter gene studies. In contrast to studies with the potato GST gene PRP, MtGst 1 promoter activity was detected in all zones of the root cortex colonized by Glomus intraradices, but nowhere else.

scholarly journals Nodule Inception Is Not Required for Arbuscular Mycorrhizal Colonization of Medicago truncatula

Plants ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 71
Author(s):  
Anil Kumar ◽  
Donna R. Cousins ◽  
Cheng-Wu Liu ◽  
Ping Xu ◽  
Jeremy D. Murray
Keyword(s):  
Arbuscular Mycorrhiza ◽  
Medicago Truncatula ◽  
Time Course ◽  
Phylogenetic Analyses ◽  
Arbuscular Mycorrhizal ◽  
Land Plants ◽  
Altered Expression ◽  
Mycorrhiza Formation ◽  
Mycorrhizal Roots ◽  
Time Course Study

Most legumes can engage in symbiosis with N-fixing bacteria called rhizobia. This symbiosis, called nodulation, evolved from the more widespread symbiosis that most land plants form with arbuscular mycorrhiza, which is reflected in a common requirement of certain genes for both these symbioses. One key nodulation gene, Nodule Inception (NIN), has been intensively studied. Mutants in NIN are unable to form nodules, which has made it difficult to identify downstream genes under the control of NIN. The analysis of data from our recent transcriptomics study revealed that some genes with an altered expression of nin during nodulation are upregulated in mycorrhizal roots. In addition, another study reported the decreased colonization of nin roots by arbuscular mycorrhiza. We therefore investigated a role for NIN in mycorrhiza formation. Our time course study, using two nin alleles with differing genetic backgrounds, suggests that that loss of NIN does not affect colonization of Medicago truncatula roots, either in the presence or absence of rhizobia. This, and recent phylogenetic analyses showing that the loss of NIN is correlated with loss of nodulation in the FaFaCuRo clade, but not with the ability to form mycorrhiza, argue against NIN being required for arbuscular mycorrhization in legumes.

scholarly journals The Medicago truncatula GRAS protein RAD1 supports arbuscular mycorrhiza symbiosis and Phytophthora palmivora susceptibility

2017 ◽  
Vol 68 (21-22) ◽  
pp. 5871-5881 ◽  
Author(s):  
Thomas Rey ◽  
Maxime Bonhomme ◽  
Abhishek Chatterjee ◽  
Aleksandr Gavrin ◽  
Justine Toulotte ◽  
...  
Keyword(s):  
Arbuscular Mycorrhiza ◽  
Medicago Truncatula ◽  
Phytophthora Palmivora ◽  
Gras Protein

scholarly journals Transcriptome Profiling in Root Nodules and Arbuscular Mycorrhiza Identifies a Collection of Novel Genes Induced During Medicago truncatula Root Endosymbioses

2004 ◽  
Vol 17 (10) ◽  
pp. 1063-1077 ◽  
Author(s):  
Katja Manthey ◽  
Franziska Krajinski ◽  
Natalija Hohnjec ◽  
Christian Firnhaber ◽  
Alfred Pühler ◽  
...  
Keyword(s):  
Arbuscular Mycorrhiza ◽  
Medicago Truncatula ◽  
In Silico ◽  
Root Nodules ◽  
Expression Patterns ◽  
Cdna Array ◽  
Transcriptome Profiling ◽  
Transgenic Roots ◽  
Genes Encoding ◽  
Induced Genes

Transcriptome profiling based on cDNA array hybridizations and in silico screening was used to identify Medicago truncatula genes induced in both root nodules and arbuscular mycorrhiza (AM). By array hybridizations, we detected several hundred genes that were upregulated in the root nodule and the AM symbiosis, respectively, with a total of 75 genes being induced during both interactions. The second approach based on in silico data mining yielded several hundred additional candidate genes with a predicted symbiosis-enhanced expression. A subset of the genes identified by either expression profiling tool was subjected to quantitative real-time reverse-transcription polymerase chain reaction for a verification of their symbiosis-induced expression. That way, induction in root nodules and AM was confirmed for 26 genes, most of them being reported as symbiosis-induced for the first time. In addition to delivering a number of novel symbiosis-induced genes, our approach identified several genes that were induced in only one of the two root endo-symbioses. The spatial expression patterns of two symbiosis-induced genes encoding an annexin and a β-tubulin were characterized in transgenic roots using promoter-reporter gene fusions.

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