scholarly journals Nitrogen Supply Influences Herbivore-Induced Direct and Indirect Defenses and Transcriptional Responses in Nicotiana attenuata

2004 ◽  
Vol 135 (1) ◽  
pp. 496-506 ◽  
Author(s):  
Yonggen Lou ◽  
Ian T. Baldwin
Keyword(s):  
Nitrogen Supply ◽  
Nicotiana Attenuata ◽  
Transcriptional Responses ◽  
Indirect Defenses

Ecophysiological comparison of direct and indirect defenses in Nicotiana attenuata

Oecologia ◽  
2000 ◽  
Vol 124 (3) ◽  
pp. 408-417 ◽  
Author(s):  
R. Halitschke ◽  
A. Keßler ◽  
J. Kahl ◽  
A. Lorenz ◽  
I. T. Baldwin
Keyword(s):  
Nicotiana Attenuata ◽  
Indirect Defenses

Distinct physiological and transcriptional responses of leaves of paper mulberry (Broussonetia kazinoki × B. papyrifera) under different nitrogen supply levels

2020 ◽  
Vol 40 (5) ◽  
pp. 667-682
Author(s):  
Jianwei Ni ◽  
Shang Su ◽  
Hui Li ◽  
Yonghang Geng ◽  
Houjun Zhou ◽  
...  
Keyword(s):  
Nitrogen Metabolism ◽  
Expression Patterns ◽  
Sugar Metabolism ◽  
Glutamate Synthase ◽  
Amino Sugar ◽  
Nitrogen Supply ◽  
Inorganic Pyrophosphatase ◽  
Transcriptional Responses ◽  
Nitrogenous Compounds ◽  
Nitrogen Concentrations

Abstract Paper mulberry, a vigorous pioneer species used for ecological reclamation and a high-protein forage plant for economic development, has been widely planted in China. To further develop its potential value, it is necessary to explore the regulatory mechanism of nitrogen metabolism for rational nitrogen utilization. In this study, we investigated the morphology, physiology and transcriptome of a paper mulberry hybrid (Broussonetia kazinoki × B. papyrifera) in response to different nitrogen concentrations. Moderate nitrogen promoted plant growth and biomass accumulation. Photosynthetic characteristics, concentration of nitrogenous compounds and activities of enzymes were stimulated under nitrogen treatment. However, these enhancements were slightly or severely inhibited under excessive nitrogen supply. Nitrite reductase and glutamate synthase were more sensitive than nitrate reductase and glutamine synthetase and more likely to be inhibited under high nitrogen concentrations. Transcriptome analysis of the leaf transcriptome identified 161,961 unigenes. The differentially expressed genes associated with metabolism of nitrogen, alanine, aspartate, glutamate and glycerophospholipid showed high transcript abundances after nitrogen application, whereas those associated with glycerophospholipid, glycerolipid, amino sugar and nucleotide sugar metabolism were down-regulated. Combined with weighted gene coexpression network analysis, we uncovered 16 modules according to similarity in expression patterns. Asparagine synthetase and inorganic pyrophosphatase were considered two hub genes in two modules, which were associated with nitrogen metabolism and phosphorus metabolism, respectively. The expression characteristics of these genes may explain the regulation of morphological, physiological and other related metabolic strategies harmoniously. This multifaceted study provides valuable insights to further understand the mechanism of nitrogen metabolism and to guide utilization of paper mulberry.

Nitrogen distribution in young Norway spruce (Picea abies) trees as affected by pedospheric nitrogen supply

1997 ◽  
Vol 101 (4) ◽  
pp. 764-769 ◽  
Author(s):  
Heike Stoermer ◽  
Bettina Seith ◽  
Ulrike Hanemann ◽  
Eckhard George ◽  
Heinz Rennenberg
Keyword(s):  
Picea Abies ◽  
Norway Spruce ◽  
Nitrogen Supply ◽  
Nitrogen Distribution

Influence of increased nutrient supply on Microcystis aeruginosa at cellular and proteomic levels

2020 ◽  
Vol 85 ◽  
pp. 47-58
Author(s):  
Y Jiang ◽  
Y Liu
Keyword(s):  
Microcystis Aeruginosa ◽  
Regulatory Protein ◽  
Acid Synthesis ◽  
Nutrient Supply ◽  
Nitrogen Supply ◽  
Nitrogen And Phosphorus ◽  
Comprehensive Description ◽  
Amino Acid Synthesis ◽  
High Nutrient ◽  
Proteomic Responses

Various studies have observed that increased nutrient supply promotes the growth of bloom-forming cyanobacteria, but only a limited number of studies have investigated the influence of increased nutrient supply on bloom-forming cyanobacteria at the proteomic level. We investigated the cellular and proteomic responses of Microcystis aeruginosa to elevated nitrogen and phosphorus supply. Increased supply of both nutrients significantly promoted the growth of M. aeruginosa and the synthesis of chlorophyll a, protein, and microcystins. The release of microcystins and the synthesis of polysaccharides negatively correlated with the growth of M. aeruginosa under high nutrient levels. Overexpressed proteins related to photosynthesis, and amino acid synthesis, were responsible for the stimulatory effects of increased nutrient supply in M. aeruginosa. Increased nitrogen supply directly promoted cyanobacterial growth by inducing the overexpression of the cell division regulatory protein FtsZ. NtcA, that regulates gene transcription related to both nitrogen assimilation and microcystin synthesis, was overexpressed under the high nitrogen condition, which consequently induced overexpression of 2 microcystin synthetases (McyC and McyF) and promoted microcystin synthesis. Elevated nitrogen supply induced the overexpression of proteins involved in gas vesicle organization (GvpC and GvpW), which may increase the buoyancy of M. aeruginosa. Increased phosphorus level indirectly affected growth and the synthesis of cellular substances in M. aeruginosa through the mediation of differentially expressed proteins related to carbon and phosphorus metabolism. This study provides a comprehensive description of changes in the proteome of M. aeruginosa in response to an increased supply of 2 key nutrients.

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