scholarly journals Effects on Photosystem II Function, Photoinhibition, and Plant Performance of the Spontaneous Mutation of Serine-264 in the Photosystem II Reaction Center D1 Protein in Triazine-Resistant Brassica napus L

1993 ◽  
Vol 103 (1) ◽  
pp. 105-113 ◽  
Author(s):  
C. Sundby ◽  
W. S. Chow ◽  
J. M. Anderson
Keyword(s):  
Brassica Napus ◽  
Photosystem Ii ◽  
Reaction Center ◽  
Spontaneous Mutation ◽  
D1 Protein ◽  
Plant Performance ◽  
Brassica Napus L

Breakdown of the photosystem II reaction center D1 protein under photoinhibitory conditions: identification and localization of the C-terminal degradation products

Biochemistry ◽  
1991 ◽  
Vol 30 (42) ◽  
pp. 10220-10226 ◽  
Author(s):  
Roberto Barbato ◽  
Giulia Friso ◽  
Maria Teresa Giardi ◽  
Fernanda Rigoni ◽  
Giorgio Mario Giacometti
Keyword(s):  
Photosystem Ii ◽  
Reaction Center ◽  
Degradation Products ◽  
D1 Protein

scholarly journals Formation of DEG5 and DEG8 Complexes and Their Involvement in the Degradation of Photodamaged Photosystem II Reaction Center D1 Protein in Arabidopsis

2007 ◽  
Vol 19 (4) ◽  
pp. 1347-1361 ◽  
Author(s):  
Xuwu Sun ◽  
Lianwei Peng ◽  
Jinkui Guo ◽  
Wei Chi ◽  
Jinfang Ma ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Reaction Center ◽  
D1 Protein

Fine mapping of the epistatic suppressor gene (esp) of a recessive genic male sterility in rapeseed (Brassica napus L.)

Genome ◽  
2009 ◽  
Vol 52 (9) ◽  
pp. 755-760 ◽  
Author(s):  
Zhenghua Xu ◽  
Yanzhou Xie ◽  
Dengfeng Hong ◽  
Pingwu Liu ◽  
Guangsheng Yang
Keyword(s):  
Brassica Napus ◽  
Male Sterility ◽  
Fine Mapping ◽  
Ssr Marker ◽  
Scar Marker ◽  
Spontaneous Mutation ◽  
Suppressor Gene ◽  
Brassica Napus L ◽  
Genic Male Sterility ◽  
Recessive Genic Male Sterility

9012AB, a recessive genic male sterility (RGMS) line derived from spontaneous mutation in Brassica napus , has been playing an important role in rapeseed hybrid production in China. The male sterility of 9012AB is controlled by two recessive genes (ms3 and ms4) interacting with one recessive epistatic suppressor gene (esp). The objective of this study was to develop PCR-based markers tightly linked to the esp gene and construct a high-resolution map surrounding the esp gene. From the survey of 512 AFLP primer combinations, 3 tightly linked AFLP markers were obtained and successfully converted to codominant or dominant SCAR markers. Furthermore, a codominant SSR marker (Ra2G08) associated with the esp gene was identified through genetic map integration. For fine mapping of the esp gene, these PCR-based markers were analyzed in a large BC1 population of 2545 plants. The esp gene was then genetically restricted to a region of 1.03 cM, 0.35 cM from SSR marker Ra2G08 and 0.68 cM from SCAR marker WSC6. The SCAR marker WSC5 co-segregated with the target gene. These results lay a solid foundation for map-based cloning of esp and will facilitate the selection of RGMS lines and their temporary maintainers.

Photoinactivation of Photosystem II and Degradation of the D1 Protein are Reduced in a Cytochrome b6/f -Less Mutant of Chlamydomonas reinhardtii

1990 ◽  
Vol 45 (5) ◽  
pp. 395-401 ◽  
Author(s):  
Susana Shochat ◽  
Noam Adir ◽  
Alma Gal ◽  
Yorinao Inoue ◽  
Laurence Mets ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Chlamydomonas Reinhardtii ◽  
Reaction Center ◽  
Electron Flow ◽  
D1 Protein ◽  
Charge Recombination ◽  
Cyt B ◽  
Variable Fluorescence ◽  
Cytochrome B6 ◽  
Mutant Cells

Abstract The effect of unoccupancy of the QB site by plastoquinone on the photoinactivation of reaction center II in a Cyt b6/f-less mutant of Chlamydomonas reinhardtii, B6, was investigated. In these cells the oxidation of plastoquinol generated by electron flow via RC II to plastoquinone and thus the turnover of PQH2/PQ via the QB site are drastically reduced. Reaction center II of the mutant cells was resistant to photoinactivation relative to the control cells as demonstrated by measurements of light-induced destabilization of S2-QB charge recombination, rise in in­ trinsic fluorescence and loss of variable fluorescence. These parameters relate to functions in­ volving the reaction center II D1 protein. The light-induced degradation of D1 in the mutant cells was also considerably reduced, with a t 1/2 value of 7 h as compared, under similar conditions, to about 1.5 h for the control cells. These results indicate that the photoinactivation of RC II and turnover of the D1 protein are related and require occupancy of the QB site by PQ and its light-driven reduction.

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