scholarly journals Characterization of Geraniol Synthase from the Peltate Glands of Sweet Basil

2003 ◽  
Vol 134 (1) ◽  
pp. 370-379 ◽  
Author(s):  
Yoko Iijima ◽  
David R. Gang ◽  
Eyal Fridman ◽  
Efraim Lewinsohn ◽  
Eran Pichersky
Keyword(s):  
Geraniol Synthase ◽  
Sweet Basil

scholarly journals Characterization of some compounds isolated from Sweet Basil (Ocimum basilicum L.) leaf extract

2016 ◽  
Vol 2 (7) ◽  
pp. 159
Author(s):  
Hadush Gebrehiwot ◽  
Aman Dekebo ◽  
Rakesh Kumar Bachheti
Keyword(s):  
Crude Extract ◽  
Ocimum Basilicum ◽  
Spectroscopic Techniques ◽  
Traditional Medicines ◽  
Sweet Basil ◽  
H Nmr ◽  
The Family ◽  
Pure Compounds ◽  
Chloroform Methanol

<p class="abstract"><strong>Background: </strong>For thousands of years, several people depend on traditional medicines from flowers, bark, leaves and fruits of plants. Sweet Basil (<em>Ocimum basilicum L.</em>) is a medicinal herb which belongs to the family of Labiatae and it is the most abundant of the genus <em>Ocimum.</em> The present study aimed to isolate pure compounds from chloroform/methanol (1:1) crude extract of the leaves of <em>O. basilicum </em>herb and characterize them using infra-red (IR) and nuclear magnetic resonance (NMR) spectroscopic techniques.</p><p class="abstract"><strong>Methods:</strong> The sample of powdered leaves of <em>O. basilicum </em>was extracted using a mixture of chloroform and methanol (1:1) and the crude extract was investigated for its chemical composition using spectroscopic techniques.</p><p class="abstract"><strong>Results:</strong> Only one fraction designated as <em>O. basilicum </em>-14 (OB-14) displayed a single spot on TLC plate. Results showed that two isomeric compounds named <em>(1-(2-vinylcyclohexa-1,4-dienyl) propan-2-ol and 2-(2-vinylcyclohexa-1,5-dienyl) propan-1-ol) </em>were isolated as a mixtures using column chromatography over silica gel. The structures of these compounds were identified using IR and one dimensional NMR spectroscopic techniques such as proton NMR (<sup>1</sup>H NMR), carbon-13 NMR (<sup>13</sup>C NMR) and distortionless enhancement by polarization transfer (DEPT).</p><p class="abstract"><strong>Conclusions:</strong> So, more compounds can be isolated from the plant using different chromatographic techniques.</p>

scholarly journals Characterization of Phenylpropene O-Methyltransferases from Sweet Basil

2002 ◽  
Vol 14 (2) ◽  
pp. 505-519 ◽  
Author(s):  
David R. Gang ◽  
Noa Lavid ◽  
Chloe Zubieta ◽  
Feng Chen ◽  
Till Beuerle ◽  
...  
Keyword(s):  
Sweet Basil

scholarly journals Functional Characterization of a Dendrobium officinale Geraniol Synthase DoGES1 Involved in Floral Scent Formation

2020 ◽  
Vol 21 (19) ◽  
pp. 7005
Author(s):  
Conghui Zhao ◽  
Zhenming Yu ◽  
Jaime A. Teixeira da Silva ◽  
Chunmei He ◽  
Haobin Wang ◽  
...  
Keyword(s):  
Nicotiana Benthamiana ◽  
Floral Scent ◽  
Functional Characterization ◽  
Dendrobium Officinale ◽  
Transcript Levels ◽  
Geraniol Synthase ◽  
Medicinal Orchid

Floral scent is a key ornamental trait that determines the quality and commercial value of orchids. Geraniol, an important volatile monoterpene in orchids that attracts pollinators, is also involved in responses to stresses but the geraniol synthase (GES) responsible for its synthesis in the medicinal orchid Dendrobium officinale has not yet been identified. In this study, three potential geraniol synthases were mined from the D. officinale genome. DoGES1, which was localized in chloroplasts, was characterized as a geraniol synthase. DoGES1 was highly expressed in flowers, especially in petals. DoGES1 transcript levels were high in the budding stage of D. officinale flowers at 11:00 a.m. DoGES1 catalyzed geraniol in vitro, and transient expression of DoGES1 in Nicotiana benthamiana leaves resulted in the accumulation of geraniol in vivo. These findings on DoGES1 advance our understanding of geraniol biosynthesis in orchids, and lay the basis for genetic modification of floral scent in D. officinale or in other ornamental orchids.

Synthesis and characterization of nanoliposome containing Fe2+ element: A superior nano-fertilizer for ferrous iron delivery to sweet basil

2021 ◽  
Vol 283 ◽  
pp. 110110
Author(s):  
Helale Kaboli Farshchi ◽  
Majid Azizi ◽  
Manouchehr Teymouri ◽  
Amin Reza Nikpoor ◽  
Mahmoud Reza Jaafari
Keyword(s):  
Ferrous Iron ◽  
Synthesis And Characterization ◽  
Sweet Basil ◽  
Iron Delivery

scholarly journals Sweet Basil Has Distinct Synthases for Eugenol Biosynthesis in Glandular Trichomes and Roots with Different Regulatory Mechanisms

2021 ◽  
Vol 22 (2) ◽  
pp. 681
Author(s):  
Vaishnavi Amarr Reddy ◽  
Chunhong Li ◽  
Kumar Nadimuthu ◽  
Jessica Gambino Tjhang ◽  
In-Cheol Jang ◽  
...  
Keyword(s):  
Glandular Trichomes ◽  
Functional Characterization ◽  
Rna Seq ◽  
Post Translational Modifications ◽  
E Coli ◽  
Sweet Basil ◽  
Specific Regulation

Production of a volatile phenylpropene; eugenol in sweet basil is mostly associated with peltate glandular trichomes (PGTs) found aerially. Currently only one eugenol synthase (EGS), ObEGS1 which belongs to PIP family is identified from sweet basil PGTs. Reports of the presence of eugenol in roots led us to analyse other EGSs in roots. We screened for all the PIP family reductase transcripts from the RNA-Seq data. In vivo functional characterization of all the genes in E. coli showed their ability to produce eugenol and were termed as ObEGS2-8. Among all, ObEGS1 displayed highest expression in PGTs and ObEGS4 in roots. Further, eugenol was produced only in the roots of soil-grown plants, but not in roots of aseptically-grown plants. Interestingly, eugenol production could be induced in roots of aseptically-grown plants under elicitation suggesting that eugenol production might occur as a result of environmental cues in roots. The presence of ObEGS4 transcript and protein in aseptically-grown plants indicated towards post-translational modifications (PTMs) of ObEGS4. Bioinformatics analysis showed possibility of phosphorylation in ObEGS4 which was further confirmed by in vitro experiment. Our study reveals the presence of multiple eugenol synthases in sweet basil and provides new insights into their diversity and tissue specific regulation.

Characterization of the plastidial geraniol synthase from Madagascar periwinkle which initiates the monoterpenoid branch of the alkaloid pathway in internal phloem associated parenchyma

2013 ◽  
Vol 85 ◽  
pp. 36-43 ◽  
Author(s):  
Andrew J. Simkin ◽  
Karel Miettinen ◽  
Patricia Claudel ◽  
Vincent Burlat ◽  
Grégory Guirimand ◽  
...  
Keyword(s):  
Madagascar Periwinkle ◽  
Geraniol Synthase ◽  
Internal Phloem

scholarly journals Characterization of a sweet basil acyltransferase involved in eugenol biosynthesis

2020 ◽  
Vol 71 (12) ◽  
pp. 3638-3652 ◽  
Author(s):  
Niha Dhar ◽  
Sreelatha Sarangapani ◽  
Vaishnavi Amarr Reddy ◽  
Nadimuthu Kumar ◽  
Deepa Panicker ◽  
...  
Keyword(s):  
Lignin Content ◽  
Glandular Trichomes ◽  
Lignin Biosynthesis ◽  
Coniferyl Alcohol ◽  
Sweet Basil ◽  
Transgenic Lines ◽  
Common Substrate ◽  
Total Lignin

Abstract Sweet basil (Ocimum basilicum) plants produce its characteristic phenylpropene-rich essential oil in specialized structures known as peltate glandular trichomes (PGTs). Eugenol and chavicol are the major phenylpropenes produced by sweet basil varieties whose synthetic pathways are not fully elucidated. Eugenol is derived from coniferyl acetate by a reaction catalysed by eugenol synthase. An acyltransferase is proposed to convert coniferyl alcohol to coniferyl acetate which is the first committed step towards eugenol synthesis. Here, we perform a comparative next-generation transcriptome sequencing of different tissues of sweet basil, namely PGT, leaf, leaf stripped of PGTs (leaf–PGT), and roots, to identify differentially expressed transcripts specific to PGT. From these data, we identified a PGT-enriched BAHD acyltransferase gene ObCAAT1 and functionally characterized it. In vitro coupled reaction of ObCAAT1 with eugenol synthase in the presence of coniferyl alcohol resulted in eugenol production. Analysis of ObCAAT1-RNAi transgenic lines showed decreased levels of eugenol and accumulation of coniferyl alcohol and its derivatives. Coniferyl alcohol acts as a common substrate for phenylpropene and lignin biosynthesis. No differences were found in total lignin content of PGTs and leaves of transgenic lines, indicating that phenylpropene biosynthesis is not coupled to lignification in sweet basil.

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