scholarly journals Dithiol Oxidant and Disulfide Reductant Dynamically Regulate the Phosphorylation of Light-Harvesting Complex II Proteins in Thylakoid Membranes

2003 ◽  
Vol 133 (1) ◽  
pp. 37-46 ◽  
Author(s):  
Päivi Martinsuo ◽  
Saijaliisa Pursiheimo ◽  
Eva-Mari Aro ◽  
Eevi Rintamäki
Keyword(s):  
Light Harvesting ◽  
Thylakoid Membranes ◽  
Light Harvesting Complex ◽  
Complex Ii ◽  
Light Harvesting Complex Ii

scholarly journals Functional domain size in aggregates of light-harvesting complex II and thylakoid membranes

2011 ◽  
Vol 1807 (9) ◽  
pp. 1022-1031 ◽  
Author(s):  
Petar H. Lambrev ◽  
Franz-Josef Schmitt ◽  
Sabine Kussin ◽  
Max Schoengen ◽  
Zsuzsanna Várkonyi ◽  
...  
Keyword(s):  
Light Harvesting ◽  
Domain Size ◽  
Thylakoid Membranes ◽  
Functional Domain ◽  
Light Harvesting Complex ◽  
Complex Ii ◽  
Light Harvesting Complex Ii

scholarly journals Diffusion of light‐harvesting complex II in the thylakoid membranes

EMBO Reports ◽  
2005 ◽  
Vol 6 (8) ◽  
pp. 782-786 ◽  
Author(s):  
Elena Consoli ◽  
Roberta Croce ◽  
David D Dunlap ◽  
Laura Finzi
Keyword(s):  
Light Harvesting ◽  
Thylakoid Membranes ◽  
Light Harvesting Complex ◽  
Complex Ii ◽  
Light Harvesting Complex Ii

scholarly journals Conformational dynamics of Light-Harvesting Complex II in a native membrane environment

2018 ◽  
Author(s):  
F. Azadi-Chegeni ◽  
M.E. Ward ◽  
G. Perin ◽  
D. Simionato ◽  
T. Morosinotto ◽  
...  
Keyword(s):  
Solid State ◽  
Nmr Spectroscopy ◽  
Light Harvesting ◽  
Conformational Dynamics ◽  
Conformational Transitions ◽  
Thylakoid Membranes ◽  
Light Harvesting Complexes ◽  
Light Harvesting Complex ◽  
Complex Ii ◽  
Light Harvesting Complex Ii

ABSTRACTPhotosynthetic light-harvesting complexes of higher plants, moss and green algae can undergo dynamic conformational transitions, which have been correlated to their ability to adapt to fluctuations in the light environment. Herein, we demonstrate the application of solid-state NMR spectroscopy on native, heterogeneous thylakoid membranes of Chlamydomonas reinhardtii (Cr) and on Cr Light-Harvesting Complex II (LHCII) in thylakoid lipid bilayers to detect LHCII conformational dynamics in its native membrane environment. We show that membrane-reconstituted LHCII contains selective sites that undergo fast, large-amplitude motions, including the phytol tails of two chlorophylls. Protein plasticity is also observed in the N-terminal stromal loop and in protein fragments facing the lumen, involving sites that stabilize the xanthophyll-cycle carotenoid violaxanthin and the two luteins. The results report on the intrinsic flexibility of LHCII pigment-protein complexes in a membrane environment, revealing putative sites for conformational switching. In thylakoid membranes, fast dynamics of protein and pigment sites is significantly reduced, which suggests that in their native organelle membranes, LHCII complexes are locked in specific conformational states.STATEMENT OF SIGNIFICANCEPhotosynthetic Light-Harvesting Complexes undergo dynamic conformational transitions that regulate the capacity of the light-harvesting antenna. We demonstrate the application of solid-state (ss)NMR spectroscopy to investigate the structural dynamics of LHCII, the most abundant LHC complex of plants and algae, in native membranes. Selective dynamic protein and pigment residues are identified that are putative sites for a conformational switch.

Macroorganisation and flexibility of thylakoid membranes

2019 ◽  
Vol 476 (20) ◽  
pp. 2981-3018 ◽  
Author(s):  
Petar H. Lambrev ◽  
Parveen Akhtar
Keyword(s):  
Light Harvesting ◽  
Current Knowledge ◽  
Three Dimensional ◽  
Photosynthetic Apparatus ◽  
Dynamic Responses ◽  
State Transitions ◽  
Photochemical Quenching ◽  
Light Harvesting Complex ◽  
Complex Ii ◽  
Light Harvesting Complex Ii

Abstract The light reactions of photosynthesis are hosted and regulated by the chloroplast thylakoid membrane (TM) — the central structural component of the photosynthetic apparatus of plants and algae. The two-dimensional and three-dimensional arrangement of the lipid–protein assemblies, aka macroorganisation, and its dynamic responses to the fluctuating physiological environment, aka flexibility, are the subject of this review. An emphasis is given on the information obtainable by spectroscopic approaches, especially circular dichroism (CD). We briefly summarise the current knowledge of the composition and three-dimensional architecture of the granal TMs in plants and the supramolecular organisation of Photosystem II and light-harvesting complex II therein. We next acquaint the non-specialist reader with the fundamentals of CD spectroscopy, recent advances such as anisotropic CD, and applications for studying the structure and macroorganisation of photosynthetic complexes and membranes. Special attention is given to the structural and functional flexibility of light-harvesting complex II in vitro as revealed by CD and fluorescence spectroscopy. We give an account of the dynamic changes in membrane macroorganisation associated with the light-adaptation of the photosynthetic apparatus and the regulation of the excitation energy flow by state transitions and non-photochemical quenching.

scholarly journals The Three Isoforms of the Light-harvesting Complex II

2004 ◽  
Vol 279 (35) ◽  
pp. 36884-36891 ◽  
Author(s):  
Joerg Standfuss ◽  
Werner Kühlbrandt
Keyword(s):  
Light Harvesting ◽  
Light Harvesting Complex ◽  
Complex Ii ◽  
Light Harvesting Complex Ii
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