scholarly journals Three Distinct Rice Cellulose Synthase Catalytic Subunit Genes Required for Cellulose Synthesis in the Secondary Wall

2003 ◽  
Vol 133 (1) ◽  
pp. 73-83 ◽  
Author(s):  
Katsuyuki Tanaka ◽  
Kazumasa Murata ◽  
Muneo Yamazaki ◽  
Katsura Onosato ◽  
Akio Miyao ◽  
...  
Keyword(s):  
Secondary Wall ◽  
Cellulose Synthase ◽  
Catalytic Subunit ◽  
Cellulose Synthesis

scholarly journals Cellulose synthase complexes display distinct dynamic behaviors during xylem transdifferentiation

2018 ◽  
Vol 115 (27) ◽  
pp. E6366-E6374 ◽  
Author(s):  
Yoichiro Watanabe ◽  
Rene Schneider ◽  
Sarah Barkwill ◽  
Eliana Gonzales-Vigil ◽  
Joseph L. Hill ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plasma Membrane ◽  
Cell Expansion ◽  
Secondary Wall ◽  
Transition Period ◽  
Cellulose Synthase ◽  
Wall Thickening ◽  
Cellulose Synthesis ◽  
Primary Wall ◽  
Dynamic Behaviors

In plants, plasma membrane-embedded CELLULOSE SYNTHASE (CESA) enzyme complexes deposit cellulose polymers into the developing cell wall. Cellulose synthesis requires two different sets of CESA complexes that are active during cell expansion and secondary cell wall thickening, respectively. Hence, developing xylem cells, which first undergo cell expansion and subsequently deposit thick secondary walls, need to completely reorganize their CESA complexes from primary wall- to secondary wall-specific CESAs. Using live-cell imaging, we analyzed the principles underlying this remodeling. At the onset of secondary wall synthesis, the primary wall CESAs ceased to be delivered to the plasma membrane and were gradually removed from both the plasma membrane and the Golgi. For a brief transition period, both primary wall- and secondary wall-specific CESAs coexisted in banded domains of the plasma membrane where secondary wall synthesis is concentrated. During this transition, primary and secondary wall CESAs displayed discrete dynamic behaviors and sensitivities to the inhibitor isoxaben. As secondary wall-specific CESAs were delivered and inserted into the plasma membrane, the primary wall CESAs became concentrated in prevacuolar compartments and lytic vacuoles. This adjustment in localization between the two CESAs was accompanied by concurrent decreased primary wall CESA and increased secondary wall CESA protein abundance. Our data reveal distinct and dynamic subcellular trafficking patterns that underpin the remodeling of the cellulose biosynthetic machinery, resulting in the removal and degradation of the primary wall CESA complex with concurrent production and recycling of the secondary wall CESAs.

scholarly journals Phenotypic effects of changes in the FTVTxK region of an Arabidopsis secondary wall cellulose synthase compared with results from analogous mutations in other isoforms

Plant Direct ◽  
2021 ◽  
Vol 5 (8) ◽  
Author(s):  
Jason N. Burris ◽  
Mohamadamin Makarem ◽  
Erin Slabaugh ◽  
Arielle Chaves ◽  
Ethan T. Pierce ◽  
...  
Keyword(s):  
Secondary Wall ◽  
Cellulose Synthase

scholarly journals Higher plants contain homologs of the bacterial celA genes encoding the catalytic subunit of cellulose synthase.

1996 ◽  
Vol 93 (22) ◽  
pp. 12637-12642 ◽  
Author(s):  
J. R. Pear ◽  
Y. Kawagoe ◽  
W. E. Schreckengost ◽  
D. P. Delmer ◽  
D. M. Stalker
Keyword(s):  
Higher Plants ◽  
Cellulose Synthase ◽  
Catalytic Subunit ◽  
Genes Encoding

scholarly journals Secondary Wall Regulating NACs Differentially Bind at the Promoter at a CELLULOSE SYNTHASE A4 Cis-eQTL

2018 ◽  
Vol 9 ◽  
Author(s):  
Jennifer R. Olins ◽  
Li Lin ◽  
Scott J. Lee ◽  
Gina M. Trabucco ◽  
Kirk J.-M. MacKinnon ◽  
...  
Keyword(s):  
Secondary Wall ◽  
Cellulose Synthase

scholarly journals Disruption of Very-Long-Chain-Fatty Acid Synthesis Has an Impact on the Dynamics of Cellulose Synthase in Arabidopsis thaliana

Plants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1599
Author(s):  
Xiaoyu Zhu ◽  
Frédérique Tellier ◽  
Ying Gu ◽  
Shundai Li
Keyword(s):  
Fatty Acid ◽  
Cell Biology ◽  
Higher Plants ◽  
Threshold Level ◽  
Cellulose Synthase ◽  
Chain Fatty Acid ◽  
Cellulose Synthesis ◽  
Cellulose Content ◽  
Long Chain Fatty Acid ◽  
Long Chain

In higher plants, cellulose is synthesized by membrane-spanning large protein complexes named cellulose synthase complexes (CSCs). In this study, the Arabidopsis PASTICCINO2 (PAS2) was identified as an interacting partner of cellulose synthases. PAS2 was previously characterized as the plant 3-hydroxy-acyl-CoA dehydratase, an ER membrane-localized dehydratase that is essential for very-long-chain-fatty acid (VLCFA) elongation. The pas2-1 mutants show defective cell elongation and reduction in cellulose content in both etiolated hypocotyls and light-grown roots. Although disruption of VLCFA synthesis by a genetic alteration had a reduction in VLCFA in both etiolated hypocotyls and light-grown roots, it had a differential effect on cellulose content in the two systems, suggesting the threshold level of VLCFA for efficient cellulose synthesis may be different in the two biological systems. pas2-1 had a reduction in both CSC delivery rate and CSC velocity at the PM in etiolated hypocotyls. Interestingly, Golgi but not post-Golgi endomembrane structures exhibited a severe defect in motility. Experiments using pharmacological perturbation of VLCFA content in etiolated hypocotyls strongly indicate a novel function of PAS2 in the regulation of CSC and Golgi motility. Through a combination of genetic, biochemical and cell biology studies, our study demonstrated that PAS2 as a multifunction protein has an important role in the regulation of cellulose biosynthesis in Arabidopsis hypocotyl.

scholarly journals A mutation in the catalytic domain of cellulose synthase 6 halts its transport to the Golgi apparatus

2019 ◽  
Vol 70 (21) ◽  
pp. 6071-6083 ◽  
Author(s):  
Sungjin Park ◽  
Bo Song ◽  
Wei Shen ◽  
Shi-You Ding
Keyword(s):  
Golgi Apparatus ◽  
Catalytic Domain ◽  
Cellulose Synthase ◽  
Cellulose Synthesis ◽  
Normal Transport

D395N in the catalytic domain of CESA6 interrupts its normal transport to the Golgi, which hampers its function in cellulose synthesis.

scholarly journals Identification of Putative Cell Wall Synthesis Genes in Betula pendula

2020 ◽  
Author(s):  
Song Chen ◽  
Xin Lin ◽  
Xiyang Zhao ◽  
Su Chen
Keyword(s):  
Cell Wall ◽  
Betula Pendula ◽  
Secondary Cell Wall ◽  
Plant Cell Wall ◽  
Gene Families ◽  
Cellulose Synthase ◽  
Wall Structure ◽  
Cellulose Synthesis ◽  
Cell Wall Synthesis ◽  
Secondary Cell Wall Synthesis

Abstract BackgroundCellulose is an essential structural component of plant cell wall and is an important resource to produce paper, textiles, bioplastics and other biomaterials. The synthesis of cellulose is among the most important but poorly understood biochemical processes, which is precisely regulated by internal and external cues.ResultsHere, we identified 46 gene models in 7 gene families which encoding cellulose synthase and related enzymes of Betula pendula, and the transcript abundance of these genes in xylem, root, leaf and flower tissues also be determined. Based on these RNA-seq data, we have identified 8 genes that most likely participate in secondary cell wall synthesis, which include 3 cellulose synthase genes and 5 cellulose synthase-like genes. In parallel, a gene co-expression network was also constructed based on transcriptome sequencing.ConclusionsIn this study, we have identified a total of 46 cell wall synthesis genes in B. pendula, which include 8 secondary cell wall synthesis genes. These analyses will help decipher the genetic information of the cell wall synthesis genes, elucidate the molecular mechanism of cellulose synthesis and understand the cell wall structure in B. pendula.

Sign in / Sign up

Export Citation Format

Share Document