scholarly journals Isolating the Arabidopsis thaliana Genes for de Novo Purine Synthesis by Suppression of Escherichia coli Mutants (I. 5[prime]-Phosphoribosyl-5-Aminoimidazole Synthetase)

1993 ◽  
Vol 102 (2) ◽  
pp. 387-399 ◽  
Author(s):  
J. F. Senecoff ◽  
R. B. Meagher
Keyword(s):  
Escherichia Coli ◽  
Arabidopsis Thaliana ◽  
De Novo ◽  
Purine Synthesis

Cloning and characterization of a cDNA encoding serine palmitoyltransferase in Arabidopsis thaliana

2000 ◽  
Vol 28 (6) ◽  
pp. 745-747 ◽  
Author(s):  
K. Tamura ◽  
H. Nishiura ◽  
J. Mori ◽  
H. Imai
Keyword(s):  
Escherichia Coli ◽  
Arabidopsis Thaliana ◽  
Cdna Clone ◽  
De Novo ◽  
Lipid Synthesis ◽  
Serine Palmitoyltransferase ◽  
Functional Identification ◽  
E Coli ◽  
Significant Production

The first and committed step in de novo sphingo-lipid synthesis is catalysed by serine palmitoyl-transferase (EC 2.3.1.50), which condenses serine and palmitoyl-CoA to form 3-ketosphinganine in a pyridoxal-5Î-phosphate-dependent reaction. We have isolated and characterized a cDNA clone from Arabidopsis thaliana that is homologous to yeast and mammalian LCB2. For a functional identification, the A. thaliana homologous cDNA was expressed in Escherichia coli which resulted in significant production of new sphinganine in E. coli cells.

scholarly journals A new folate antimetabolite, 5,10-dideaza-5,6,7,8-tetrahydrofolate is a potent inhibitor of de novo purine synthesis

1989 ◽  
Vol 264 (1) ◽  
pp. 328-333 ◽  
Author(s):  
G P Beardsley ◽  
B A Moroson ◽  
E C Taylor ◽  
R G Moran
Keyword(s):  
De Novo ◽  
Potent Inhibitor ◽  
Purine Synthesis
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