scholarly journals Treatment of Pea (Pisum sativum L.) Protoplasts with DNA-Damaging Agents Induces a 39-Kilodalton Chloroplast Protein Immunologically Related to Escherichia coli RecA

1993 ◽  
Vol 102 (1) ◽  
pp. 155-163 ◽  
Author(s):  
H. Cerutti ◽  
H. Z. Ibrahim ◽  
A. T. Jagendorf
Keyword(s):  
Escherichia Coli ◽  
Pisum Sativum ◽  
Chloroplast Protein ◽  
Dna Damaging Agents ◽  
Pisum Sativum L

scholarly journals Purification and kinetic analysis of pea (Pisum sativum L.) NADPH:protochlorophyllide oxidoreductase expressed as a fusion with maltose-binding protein in Escherichia coli

1997 ◽  
Vol 325 (1) ◽  
pp. 139-145 ◽  
Author(s):  
Giles E. M. MARTIN ◽  
Michael P. TIMKO ◽  
Helen M. WILKS
Keyword(s):  
Escherichia Coli ◽  
Pisum Sativum ◽  
Molecular Mass ◽  
Binding Protein ◽  
Maltose Binding Protein ◽  
Cell Protein ◽  
Kinetic Properties ◽  
Nadph:Protochlorophyllide Oxidoreductase ◽  
Pisum Sativum L

NADPH:protochlorophyllide oxidoreductase (POR) catalyses the light-dependent reduction of protochlorophyllide to chlorophyllide, a key reaction in the chlorophyll biosynthetic pathway. To facilitate structure–function studies, POR from pea (Pisum sativum L.) has been overexpressed in Escherichia coli as a fusion with maltose-binding protein (MBP) at 5–10% of the total soluble cell protein. The fusion protein (MBP–POR) has been purified to greater than 90% homogeneity by a two-step affinity-purification procedure. This represents the first successful overexpression and purification of a plant POR. MBP–POR was found to be active, and the kinetic properties were determined using a continuous assay in which the rate of chlorophyllide formation was measured. The Vmax was 20.6± 0.9 nmol·min-1·mg-1 and the Km values for NADPH and protochlorophyllide were 8.7±1.9 μM and 0.27±0.04 μM respectively. These results represent the first determination of the kinetic properties of a pure POR and the first report on the kinetics of POR from a dicotyledenous plant. The experiments described here demonstrate that the enzyme is not a ‘suicide’ enzyme, and the only components required for catalysis are NADPH, protochlorophyllide and light. Size-exclusion chromatography on a Superose 6 HR column indicated that MBP–POR has a molecular mass of 155 kDa (compared with the molecular mass of 80 kDa estimated by SDS/PAGE), indicating that it behaves as a dimer in solution. This is the first direct determination of the oligomerization state of POR.

Mitigation Effects of 24-Epibrassinolide and Thiourea in Field Pea (Pisum sativum L.) under Drought Stress

2018 ◽  
Vol 34 (2) ◽  
pp. 229-235 ◽  
Author(s):  
Prachi Garg ◽  
◽  
A. Hemantaranjan ◽  
Jyostnarani Pradhan ◽  
◽  
...  
Keyword(s):  
Drought Stress ◽  
Pisum Sativum ◽  
Field Pea ◽  
Pisum Sativum L

МЕТОД ЭКСПРЕСС-ОЦЕНКИ АЛЮМОТОЛЕРАНТНОСТИ У ГОРОХА ПОСЕВНОГО (Pisum sativum L.)

2015 ◽  
Vol 50 (3) ◽  
pp. 353-360
Author(s):  
М.А. ВИШНЯКОВА ◽  
◽  
Е.В. СЕМЕНОВА ◽  
И.А. КОСАРЕВА ◽  
Н.Д. КРАВЧУК ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Pisum Sativum L
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