scholarly journals The Eukaryotic Translation Initiation Factor 4E Controls Lettuce Susceptibility to the Potyvirus Lettuce mosaic virus

2003 ◽  
Vol 132 (3) ◽  
pp. 1272-1282 ◽  
Author(s):  
Valérie Nicaise ◽  
Sylvie German-Retana ◽  
Raquel Sanjuán ◽  
Marie-Pierre Dubrana ◽  
Marianne Mazier ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Translation Initiation ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Lettuce Mosaic Virus ◽  
Eukaryotic Translation Initiation Factor ◽  
Eukaryotic Translation ◽  
Eukaryotic Translation Initiation

scholarly journals Targeted Knockout of Eukaryotic Translation Initiation Factor 4E Confers Bymovirus Resistance in Winter Barley

2021 ◽  
Vol 3 ◽  
Author(s):  
Robert Eric Hoffie ◽  
Ingrid Otto ◽  
Dragan Perovic ◽  
Nagaveni Budhagatapalli ◽  
Antje Habekuß ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Translation Initiation ◽  
Winter Barley ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Yellow Mosaic Virus ◽  
Eukaryotic Translation Initiation Factor ◽  
Eukaryotic Translation ◽  
Barley Yellow Mosaic Virus ◽  
Eukaryotic Translation Initiation

The Eukaryotic Translation Initiation Factor 4E (EIF4E) is a well-known susceptibility factor for potyvirus infections in many plant species. The barley yellow mosaic virus disease, caused by the bymoviruses Barley yellow mosaic virus (BaYMV) and Barley mild mosaic virus (BaMMV), can lead to yield losses of up to 50% in winter barley. In autumn, the roots of young barley plants are infected by the soil-borne plasmodiophoraceous parasite Polymyxa graminis L. that serves as viral vector. Upon viral establishment and systemic spreading into the upper parts of the plants, yellow mosaics occur as first symptoms on leaves. In the further course of plant development, the disease entails leaf necrosis and increased susceptibility to frost damage. Thanks to the rym4 and rym5 allelic variants of the HvEIF4E gene, more than two thirds of current European winter barley cultivars are resistant to BaYMV and BaMMV. However, several strains of BaYMV and BaMMV have already overcome rym4- and rym5-mediated resistance. Accordingly, new resistance-conferring alleles are needed for barley breeding. Therefore, we performed targeted mutagenesis of the EIF4E gene by Cas9 endonuclease in BaMMV/BaYMV-susceptible winter barley cv. “Igri”. Small insertions were generated, resulting in a shift of the translational reading frame, thereby causing the loss-of-function of EIF4E. The mutations occurred in the homozygous state already in the primary mutants. Their progeny proved invariably homozygous and fully resistant to mechanical inoculation with BaMMV. EIF4E knockout plants showed normal growth habit and produced grains, yet exhibited a yield penalty.

scholarly journals Adaptation of Lettuce mosaic virus to Catharanthus roseus Involves Mutations in the Central Domain of the VPg

2014 ◽  
Vol 27 (5) ◽  
pp. 491-497 ◽  
Author(s):  
Laurence Svanella-Dumas ◽  
Eric Verdin ◽  
Chantal Faure ◽  
Sylvie German-Retana ◽  
Patrick Gognalons ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Catharanthus Roseus ◽  
Translation Initiation ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Terminal Part ◽  
Lettuce Mosaic Virus ◽  
Eukaryotic Translation ◽  
Illumina Deep Sequencing ◽  
Eukaryotic Translation Initiation

An isolate of Lettuce mosaic virus (LMV, a Potyvirus) infecting Madagascar periwinckle (Catharanthus roseus) was identified and characterized by Illumina deep sequencing. LMV-Cr has no close affinities to previously sequenced LMV isolates and represents a novel, divergent LMV clade. Inoculation experiments with other representative LMV isolates showed that they are unable to infect C. roseus, which was not known to be a host for LMV. However, three C. roseus variants of one of these isolates, LMV-AF199, could be selected and partially or completely sequenced. These variants are characterized by the accumulation of mutations affecting the C-terminal part of the cylindrical inclusion (CI) helicase and the central part of the VPg. In particular, a serine to proline mutation at amino acid 143 of the VPg was observed in all three independently selected variants and is also present in the LMV-Cr isolate, making it a prime candidate as a host-range determinant. Other mutations at VPg positions 65 and 144 could also contribute to the ability to infect C. roseus. Inoculation experiments involving a recombinant LMV expressing a permissive lettuce eukaryotic translation initiation factor 4E (eIF4E) suggest that eIF4E does not contribute to the interaction of most LMV isolates with C. roseus.

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