scholarly journals A Reevaluation of the Role of the Heterotrimeric G Protein in Coupling Light Responses in Arabidopsis

2003 ◽  
Vol 131 (4) ◽  
pp. 1623-1627 ◽  
Author(s):  
Alan M. Jones ◽  
Joseph R. Ecker ◽  
Jin-Gui Chen
Keyword(s):  
G Protein ◽  
Light Responses ◽  
Heterotrimeric G Protein

scholarly journals Role of Isoprenoid Lipids on the Heterotrimeric G Protein γ Subunit in Determining Effector Activation

1999 ◽  
Vol 274 (23) ◽  
pp. 16595-16603 ◽  
Author(s):  
Chang-Seon Myung ◽  
Hiroshi Yasuda ◽  
Wendy W. Liu ◽  
T. Kendall Harden ◽  
James C. Garrison
Keyword(s):  
G Protein ◽  
Heterotrimeric G Protein ◽  
Γ Subunit

scholarly journals Role of a Heterotrimeric G Protein in Regulation of Arabidopsis Seed Germination

2002 ◽  
Vol 129 (2) ◽  
pp. 897-907 ◽  
Author(s):  
Hemayet Ullah ◽  
Jin-Gui Chen ◽  
Shucai Wang ◽  
Alan M. Jones
Keyword(s):  
Seed Germination ◽  
G Protein ◽  
Heterotrimeric G Protein ◽  
Arabidopsis Seed

Role of the α subunit of heterotrimeric G-protein in probenazole-inducing defense signaling in rice

2003 ◽  
Vol 69 (1) ◽  
pp. 83-86 ◽  
Author(s):  
Michiaki Iwata ◽  
Kenji Umemura ◽  
Takeshi Teraoka ◽  
Hideki Usami ◽  
Yukiko Fujisawa ◽  
...  
Keyword(s):  
G Protein ◽  
Heterotrimeric G Protein ◽  
Α Subunit ◽  
Defense Signaling

scholarly journals The Role of Arabidopsis Heterotrimeric G-Protein Subunits in MLO2 Function and MAMP-Triggered Immunity

2013 ◽  
Vol 26 (9) ◽  
pp. 991-1003 ◽  
Author(s):  
Justine Lorek ◽  
Thomas Griebel ◽  
Alan M. Jones ◽  
Hannah Kuhn ◽  
Ralph Panstruga
Keyword(s):  
G Protein ◽  
G Proteins ◽  
Protein Complex ◽  
Defense Responses ◽  
Resistance Locus ◽  
Callose Deposition ◽  
Heterotrimeric G Protein ◽  
Mildew Resistance ◽  
Genetic Ablation

Heterotrimeric G-proteins, composed of Gα, Gβ, and Gγ subunits, regulate many fundamental processes in plants. In animals, ligand binding to seven transmembrane (7TM) cell surface receptors designated G-protein coupled receptors (GPCR) leads to heterotrimeric G-protein activation. Because the plant G-protein complex is constitutively active, the exact role of plant 7TM proteins in this process is unclear. Members of the mildew resistance locus O (MLO) family represent the best-characterized 7TM plant proteins. Although genetic ablation of either MLO2 or G-proteins alters susceptibility to pathogens in Arabidopsis thaliana, it is unknown whether G-proteins directly couple signaling through MLO2. Here, we exploited two well-documented phenotypes of mlo2 mutants, broad-spectrum powdery mildew resistance and spontaneous callose deposition in leaf mesophyll cells, to assess the relationship of MLO2 proteins to the G-protein complex. Although our data reveal modulation of antifungal defense responses by Gβ and Gγ subunits and a role for the Gγ1 subunit in mlo2-conditioned callose deposition, our findings overall are inconsistent with a role of MLO2 as a canonical GPCR. We discovered that mutants lacking the Gβ subunit show delayed accumulation of a subset of defense-associated genes following exposure to the microbe-associated molecular pattern flg22. Moreover, Gβ mutants were found to be hypersusceptible to spray inoculation with the bacterial pathogen Pseudomonas syringae.

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