scholarly journals Nucleotide Sequence of the Tobacco (Nicotiana tabacum) Anionic Peroxidase Gene

1993 ◽  
Vol 101 (3) ◽  
pp. 1117-1118 ◽  
Author(s):  
F. Diaz-De-Leon ◽  
K. L. Klotz ◽  
L. M. Lagrimini
Keyword(s):  
Nicotiana Tabacum ◽  
Nucleotide Sequence ◽  
Anionic Peroxidase ◽  
Peroxidase Gene

scholarly journals Nucleotide Sequence for the Genomic DNA Encoding an Anionic Peroxidase Gene from a Hybrid Poplar,Populus kitakamiensis

1993 ◽  
Vol 57 (1) ◽  
pp. 131-133 ◽  
Author(s):  
Shinya Kawai ◽  
Yasuo Matsumoto ◽  
Shinya Kajita ◽  
Keiko Yamada ◽  
Yoshihiro Katayama ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Genomic Dna ◽  
Hybrid Poplar ◽  
Dna Encoding ◽  
Anionic Peroxidase ◽  
Peroxidase Gene

scholarly journals ANIONIC PEROXIDASE GENE POLYMORPHISM IN WHEAT

2015 ◽  
Vol 176 ◽  
pp. 225-236
Author(s):  
I. V. Maksimov ◽  
◽  
G. F. Burkhanova ◽  
O. I. Kuzmina Kuzmina ◽  
V. A. Vakhitov ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Anionic Peroxidase ◽  
Peroxidase Gene

scholarly journals Nucleotide Sequence of the Nicotiana tabacum cv Xanthi Gene Encoding 1-Aminocyclopropane-1-Carboxylate Synthase

1992 ◽  
Vol 100 (3) ◽  
pp. 1615-1616 ◽  
Author(s):  
Bryan A. Bailey ◽  
Adi Avni ◽  
Ning Li ◽  
Autar K. Mattoo ◽  
James D. Anderson
Keyword(s):  
Nicotiana Tabacum ◽  
Nucleotide Sequence ◽  
Gene Encoding

scholarly journals Peroxidase Overproduction in Tomato: Wound-induced Polyphenol Deposition and Disease Resistance

HortScience ◽  
1993 ◽  
Vol 28 (3) ◽  
pp. 218-221 ◽  
Author(s):  
L. Mark Lagrimini ◽  
Jill Vaughn ◽  
W. Alan Erb ◽  
Sally A. Miller
Keyword(s):  
Disease Resistance ◽  
Nicotiana Tabacum ◽  
Lignin Content ◽  
Lignin Biosynthesis ◽  
Anionic Peroxidase ◽  
Green Fruit ◽  
Control Fruit ◽  
Epidermal Tissue ◽  
Soluble Phenols ◽  
Lignin Composition

Lignin composition in leaf, fruit, and fruit outer epidermis of transgenic tomato (Lycopersicon esculentum Mill.) plants that overproduce the enzyme tobacco anionic peroxidase (TobAnPOD) was analyzed. This enzyme may catalyze the polymerization of cinnamyl alcohols into lignin in tobacco (Nicotiana tabacum L.); therefore, we predicted that its presence in the transformed tissue would increase lignin levels in healthy and wounded tissue. Lignin levels in healthy plants increased by 20% in leaf, 49% in fruit, and 106% in fruit outer epidermal tissue. Mature-green fruit were aseptically wounded and incubated in darkness for up to 7 days. Soluble phenols in wounded transgenic fruit increased by more than 300% hut changed little in control fruit. As with soluble phenols, lignin content in wounded transformed fruit increased by more than 20-fold hut increased less than two-fold in control fruit. Transgenic seedlings overproducing TobAnPOD were screened for susceptibility to several pathogens, but resistance did not increase. Possible TobAnPOD roles in lignin biosynthesis, phenol metabolism, stress response, and disease resistance are discussed.

scholarly journals Expression of a Chimeric Tobacco Peroxidase Gene in Transgenic Tomato Plants

1992 ◽  
Vol 117 (6) ◽  
pp. 1012-1016 ◽  
Author(s):  
L. Mark Lagrimini ◽  
Jill Vaughn ◽  
John Finer ◽  
Karen Klotz ◽  
Patrick Rubaihayo
Keyword(s):  
Peroxidase Activity ◽  
Tomato Fruit ◽  
Cauliflower Mosaic Virus ◽  
Soluble Solids ◽  
Foreign Gene ◽  
Peroxidase Isoenzyme ◽  
Tomato Plants ◽  
Anionic Peroxidase ◽  
Peroxidase Gene ◽  
Tobacco Peroxidase

Tomato plants (Lycopersicon esculentum cv. OH 7814) were transformed via Agrobacterium tumefaciens with a chimeric tobacco anionic peroxidase (EC 1.11.1.7) gene joined to the cauliflower mosaic virus (CaMV) 35S promoter. Transgenic plants obtained by selection on kanamycin were found to have more than five times the total leaf peroxidase activity of control plants. Transformed tomato plants chronically wilted upon reaching sexual maturity. Two independently selected transformants were self-fertilized, and progeny were obtained that were homozygous for the foreign gene. Isoelectric focusing gels stained for peroxidase activity revealed a new tomato leaf peroxidase isoenzyme with a pI of 3.75, which is similar to that seen in Nicotiana sylvestris L. Mature tomato fruit were found to have up to 1600-fold higher peroxidase activity in transformants expressing the tobacco anionic peroxidase (TobAnPOD) than control plants. Tissue blots showed the tobacco enzyme evenly distributed throughout the tomato fruit tissue. Progeny plants possessing the tobacco peroxidase gene (now homozygous) showed stunting, and fruit size was reduced by >80%. However, fruit set was normal and the rate of ripening was not altered from control plants. Fruit from transformed plants were found to have normal pigmentation, but the soluble solids concentration was 400% higher than in control tomato fruit. This result was predicted from the peroxidase-induced water stress. Possible roles for the tobacco anionic peroxidase in growth, development, and stress resistance are discussed.

Sign in / Sign up

Export Citation Format

Share Document