scholarly journals THE EXTRACTION OF PLANT TISSUE FLUIDS AND THEIR UTILITY IN PHYSIOLOGICAL STUDIES

1926 ◽  
Vol 1 (1) ◽  
pp. 57-65 ◽  
Author(s):  
R. Newton ◽  
W. R. Brown ◽  
W. M. Martin
1922 ◽  
Vol 74 (3) ◽  
pp. 308-313 ◽  
Author(s):  
Ross Aiken Gortner ◽  
Walter F. Hoffman

1921 ◽  
Vol 18 (4) ◽  
pp. 106-109 ◽  
Author(s):  
J. A. Harris ◽  
R. A. Gortner ◽  
W. F. Hofmann ◽  
A. T. Valentine

1924 ◽  
Vol 77 (1) ◽  
pp. 96-102
Author(s):  
Ross Aiken Gortner ◽  
Walter F. Hoffman

1922 ◽  
Vol 74 (4) ◽  
pp. 442-446 ◽  
Author(s):  
Robert Newton ◽  
Ross Aiken Gortner
Keyword(s):  

Ecology ◽  
1925 ◽  
Vol 6 (4) ◽  
pp. 391-396 ◽  
Author(s):  
John V. Lawrence ◽  
J. Arthur Harris

Author(s):  
Russell L. Steere ◽  
Eric F. Erbe

It has been assumed by many involved in freeze-etch or freeze-fracture studies that it would be useless to etch specimens which were cryoprotected by more than 15% glycerol. We presumed that the amount of cryoprotective material exposed at the surface would serve as a contaminating layer and prevent the visualization of fine details. Recent unexpected freeze-etch results indicated that it would be useful to compare complementary replicas in which one-half of the frozen-fractured specimen would be shadowed and replicated immediately after fracturing whereas the complement would be etched at -98°C for 1 to 10 minutes before being shadowed and replicated.Standard complementary replica holders (Steere, 1973) with hinges removed were used for this study. Specimens consisting of unfixed virus-infected plant tissue infiltrated with 0.05 M phosphate buffer or distilled water were used without cryoprotectant. Some were permitted to settle through gradients to the desired concentrations of different cryoprotectants.


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