Erratum: Entropic Trapping and Escape of Long DNA Molecules at Submicron Size Constriction [Phys. Rev. Lett. 83, 1688 (1999)]

J. Han; S. W. Turner; H. G. Craighead

doi:10.1103/physrevlett.86.1394

Erratum: Entropic Trapping and Escape of Long DNA Molecules at Submicron Size Constriction [Phys. Rev. Lett. 83, 1688 (1999)]

Physical Review Letters ◽

10.1103/physrevlett.86.1394 ◽

2001 ◽

Vol 86 (7) ◽

pp. 1394-1394 ◽

Cited By ~ 9

Author(s):

J. Han ◽

S. W. Turner ◽

H. G. Craighead

Keyword(s):

Dna Molecules ◽

Submicron Size ◽

Entropic Trapping

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Entropic Trapping and Escape of Long DNA Molecules at Submicron Size Constriction

Physical Review Letters ◽

10.1103/physrevlett.83.1688 ◽

1999 ◽

Vol 83 (8) ◽

pp. 1688-1691 ◽

Cited By ~ 294

Author(s):

J. Han ◽

S. W. Turner ◽

H. G. Craighead

Keyword(s):

Dna Molecules ◽

Submicron Size ◽

Entropic Trapping

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Entropic trapping and sieving of long DNA molecules in a nanofluidic channel

Journal of Vacuum Science & Technology A Vacuum Surfaces and Films ◽

10.1116/1.581740 ◽

1999 ◽

Vol 17 (4) ◽

pp. 2142-2147 ◽

Cited By ~ 91

Author(s):

J. Han ◽

H. G. Craighead

Keyword(s):

Dna Molecules ◽

Entropic Trapping

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Replication of Bacteriophage 186 DNA

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010009422x ◽

1972 ◽

Vol 30 ◽

pp. 194-195

Author(s):

Dhruba K. Chattoraj ◽

Ross B. Inman

Keyword(s):

Electron Microscopy ◽

Dna Replication ◽

Frame Of Reference ◽

Dna Molecules ◽

E Coli ◽

Phage Dna ◽

Partial Denaturation

Electron microscopy of replicating intermediates has been quite useful in understanding the mechanism of DNA replication in DNA molecules of bacteriophage, mitochondria and plasmids. The use of partial denaturation mapping has made the tool more powerful by providing a frame of reference by which the position of the replicating forks in bacteriophage DNA can be determined on the circular replicating molecules. This provided an easy means to find the origin and direction of replication in λ and P2 phage DNA molecules. DNA of temperate E. coli phage 186 was found to have an unique denaturation map and encouraged us to look into its mode of replication.

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Synchrony of Digestion of SV40 DNA by Exonuclease III Determined by EM

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100116991 ◽

1975 ◽

Vol 33 ◽

pp. 674-675

Author(s):

Ray Wu ◽

G. Ruben ◽

B. Siegel ◽

P. Spielman ◽

E. Jay

Keyword(s):

Dark Field ◽

Uranyl Acetate ◽

Enzyme Digestion ◽

Dna Molecules ◽

Exonuclease Iii ◽

Aluminum Films ◽

Double Stranded Dna ◽

Before And After ◽

Exo Iii ◽

Sv40 Dna

A method for determining long nucleotide sequences of double-stranded DNA is being developed. It involves (a) the synchronous digestion of the DNA from the 3' ends with EL coli exonuclease III (Exo III) followed by (b) resynthesis with labeled nucleotides and DNA polymerase. A crucial factor in the success of this method is the degree to which the enzyme digestion proceeds synchronously under proper conditions of incubation (step a). Dark field EM is used to obtain accurate measurements on the lengths and distribution of the DNA molecules before and after digestion with Exo III, while gel electrophoresis is used in parallel to obtain a mean length for these molecules. It is the measurements on a large enough sample of individual molecules by EM that provides the information on how synchronously the digestion proceeds. For length measurements, the DNA molecules were picked up on 20-30 Å thick carbon-aluminum films, using the aqueous Kleinschmidt technique and stained with 7.5 x 10-5M uranyl acetate in 90% ethanol for 3 minutes.

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