scholarly journals Convective dispersion of particles in a segmented flow

2019 ◽  
Vol 4 (10) ◽  
Author(s):  
Wafa Bouhlel ◽  
S. Danial Naghib ◽  
Jérôme Bibette ◽  
Nicolas Bremond
Chemosensors ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 122
Author(s):  
Aldo Roda ◽  
Pierpaolo Greco ◽  
Patrizia Simoni ◽  
Valentina Marassi ◽  
Giada Moroni ◽  
...  

A simple and versatile continuous air-segmented flow sensor using immobilized luciferase was designed as a general miniaturized platform based on sensitive biochemiluminescence detection. The device uses miniaturized microperistaltic pumps to deliver flows and compact sensitive light imaging detectors based on BI-CMOS (smartphone camera) or CCD technology. The low-cost components and power supply make it suitable as out-lab device at point of need to monitor kinetic-related processes or ex vivo dynamic events. A nylon6 flat spiral carrying immobilized luciferase was placed in front of the detector in lensless mode using a fiber optic tapered faceplate. ATP was measured in samples collected by microdialysis from rat brain with detecting levels as low as 0.4 fmoles. The same immobilized luciferase was also used for the evaluation of bile salt hydrolase (BSH) activity in intestinal microbiota. An aminoluciferin was conjugatated with chenodeoxycholic acid forming the amide derivative aLuc-CDCA. The hydrolysis of the aLuc-CDCA probe by BSH releases free uncaged aminoluciferin which is the active substrate for luciferase leading to light emission. This method can detect as low as 0.5 mM of aLuc-CDCA, so it can be used on real faecal human samples to study BSH activity and its modulation by diseases and drugs.


2019 ◽  
Vol 207 ◽  
pp. 1288-1298 ◽  
Author(s):  
Maren Termühlen ◽  
Benedikt Strakeljahn ◽  
Gerhard Schembecker ◽  
Kerstin Wohlgemuth
Keyword(s):  

2017 ◽  
Vol 122 (1) ◽  
pp. 48-51 ◽  
Author(s):  
R. C. Mbwahnche ◽  
L. B. Matyushkin ◽  
O. A. Ryzhov ◽  
O. A. Aleksandrova ◽  
V. A. Moshnikov

2015 ◽  
Vol 54 (17) ◽  
pp. 4699-4708 ◽  
Author(s):  
Anne-Kathrin Liedtke ◽  
Frederik Scheiff ◽  
Frédéric Bornette ◽  
Régis Philippe ◽  
David W. Agar ◽  
...  

2017 ◽  
Vol 12 (2) ◽  
Author(s):  
Veronika Kmecl ◽  
Tea Knap ◽  
Dragan Žnidarčič

Nitrate (NO<sub>3</sub><sup>–</sup>) and nitrite (NO<sub>2</sub><sup>–</sup>) levels of a total 1195 samples of nine different vegetables (lettuce, potato, cabbage, carrot, string beans, tomato, cucumber, cauliflower and pepper) collected at several locations of an intensive agricultural area in Slovenia were analysed during a period of 13 years. The content of NO<sub>2</sub><sup>–</sup> and NO<sub>3</sub><sup>–</sup> ions in commercial mature samples was determined using a segmented flow analyser. The average NO<sub>3</sub><sup>–</sup> content was the highest in lettuce (962 mg/kg), cabbage (795 mg/kg), string beans (298 mg/kg), carrot (264 mg/kg), cauliflower (231 mg/kg), potato (169 mg/kg) and was moderately high in cucumber (93 mg/kg) and pepper (69 mg/kg). A low NO<sub>3</sub><sup>–</sup> content was found in tomato (2<sup>–</sup> did not exceed 0.5 mg/kg, with the exception of potato (1.08 mg/kg). Six samples of lettuce exceeded the maximum permissible level of NO<sub>3</sub><sup>–</sup> according to current European Union (EU) legislation. Based on the results of our investigation, we assessed the approximate daily intake (DI) of NO<sub>3</sub>– and NO<sub>2</sub><sup>–</sup> to human body. The results indicated that with the consumption of potato, the daily intake per inhabitant is close to the acceptable DI permitted in EU.


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