Manipulation and Assessment of Human Red Blood Cells with Tunable “Tug-of-War” Optical Tweezers

2019 ◽  
Vol 12 (6) ◽  
Author(s):  
Yi Liang ◽  
Guo Liang ◽  
Yinxiao Xiang ◽  
Josh Lamstein ◽  
Rekha Gautam ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Optical Tweezers ◽  
Blood Cells ◽  
Human Red Blood Cells

Manipulation on human red blood cells with femtosecond optical tweezers

2008 ◽  
Vol 6 (12) ◽  
pp. 919-921 ◽  
Author(s):  
杨海峰 Ming Zhou ◽  
周明 Haifeng Yang ◽  
狄建科 Jianke Di ◽  
赵恩兰 Enlan Zhao
Keyword(s):  
Red Blood Cells ◽  
Optical Tweezers ◽  
Blood Cells ◽  
Human Red Blood Cells

THE INVERSE PROBLEM OF RED BLOOD CELLS DEFORMED BY OPTICAL TWEEZERS

2011 ◽  
Vol 08 (03) ◽  
pp. 483-492
Author(s):  
X. Z. ZHOU ◽  
F. P. ZHAO ◽  
Z. H. SUN ◽  
H. A. WU
Keyword(s):  
Inverse Problem ◽  
Finite Element ◽  
Red Blood Cells ◽  
Optical Tweezers ◽  
Blood Cells ◽  
Constitutive Models ◽  
Finite Element Simulations ◽  
Human Red Blood Cells ◽  
Yeoh Model ◽  
Constitutive Parameters

Optical tweezers are widely used to study the mechanical properties of human red blood cells. This paper examines the inverse problem of computing constitutive parameters from the experimental loading-response data. Hyperelastic constitutive models are employed to characterize the stress–strain relationship of red blood cells. The large deformation and evolution of stress in red blood cells under different tensile loadings are investigated using finite element simulations. The results show that the Yeoh model provides a better characterization of human red blood cells. A nonlinear regression analysis method is presented to derive hyperelastic parameters from the experimental results. The obtained constitutive model and parameters are validated by comparing the force–displacement curves from finite element simulations and from experimental data.

scholarly journals Lateral Deformation of Human Red Blood Cells by Optical Tweezers

Micromachines ◽  
2021 ◽  
Vol 12 (9) ◽  
pp. 1024
Author(s):  
Pavel Yale ◽  
Michel A. Kouacou ◽  
Jean-Michel E. Konin ◽  
Eugène Megnassan ◽  
Jérémie T. Zoueu
Keyword(s):  
Red Blood Cells ◽  
Optical Tweezers ◽  
Blood Cells ◽  
Ccd Camera ◽  
Shear Stiffness ◽  
Lateral Force ◽  
Elastic Stiffness ◽  
Lateral Deformation ◽  
Mean Values ◽  
Human Red Blood Cells

In this paper, we studied the lateral deformation of human red blood cells (RBCs) during lateral indentation by an optically trapped silica bead with a diameter of 4.5 µm (Bangs Laboratories, Inc. Fishers, IN, USA). The images were captured using a CCD camera and the Boltzmann statistics method was used for force calibration. Using the Hertz model, we calculated and compared the elastic stiffness resulting from the lateral force, showing that the differences are important and that the force should be considered. Besides the lateral component, this setup also allowed us to examine the lateral cell–bead interaction. The mean values of the cell shear stiffness measured during indentation were 3.37 ± 0.40 µN/m for biconcave RBCs, 3.48 ± 0.23 µN/m for spherical RBCs, and 3.80 ± 0.22 µN/m for crenelated RBCs, respectively. These results show that this approach can be used as a routine method for RBC study, because it enabled us to manipulate the cell without contact with the wall.

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