High-Throughput Microscopy Using Live Mammalian Cells

2010 ◽  
Vol 2010 (8) ◽  
pp. pdb.top84 ◽  
Author(s):  
Stefan Terjung ◽  
Thomas Walter ◽  
Arne Seitz ◽  
Beate Neumann ◽  
Rainer Pepperkok ◽  
...  
Keyword(s):  
High Throughput ◽  
Mammalian Cells

A High-Throughput System for Transient and Stable Protein Production in Mammalian Cells

2019 ◽  
pp. 93-142 ◽  
Author(s):  
Sarah M. Rue ◽  
Paul W. Anderson ◽  
Michelle R. Gaylord ◽  
Jessica J. Miller ◽  
Scott M. Glaser ◽  
...  
Keyword(s):  
High Throughput ◽  
Mammalian Cells ◽  
Protein Production

Photothermal nanoblades for delivery of large-sized cargo into mammalian cells at high throughput

2016 ◽  
Author(s):  
Yi-Chien Wu ◽  
Tuhin Santra ◽  
Ting-Hsiang Wu ◽  
Daniel L. Clemens ◽  
Bai-Yu Lee ◽  
...  
Keyword(s):  
High Throughput ◽  
Mammalian Cells

scholarly journals Semicontinuous Flow Electroporation Chip for High-Throughput Transfection on Mammalian Cells

2009 ◽  
Vol 81 (11) ◽  
pp. 4414-4421 ◽  
Author(s):  
Shengnian Wang ◽  
Xulang Zhang ◽  
Weixiong Wang ◽  
L. James Lee
Keyword(s):  
High Throughput ◽  
Mammalian Cells

scholarly journals A high-throughput, flow cytometry-based method to quantify DNA-end resection in mammalian cells

2012 ◽  
Vol 81A (10) ◽  
pp. 922-928 ◽  
Author(s):  
Josep V. Forment ◽  
Rachael V. Walker ◽  
Stephen P. Jackson
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
Mammalian Cells ◽  
Dna End Resection ◽  
End Resection

Surprising roles for phospholipid binding proteins revealed by high throughput geneticsThis paper is one of a selection of papers published in this special issue entitled “Second International Symposium on Recent Advances in Basic, Clinical, and Social Medicine” and has undergone the Journal's usual peer review process.

2010 ◽  
Vol 88 (4) ◽  
pp. 565-574 ◽  
Author(s):  
Marissa A. LeBlanc ◽  
Christopher R. McMaster
Keyword(s):  
High Throughput ◽  
Mammalian Cells ◽  
Binding Proteins ◽  
Essential Gene ◽  
Peer Review Process ◽  
Vesicular Transport ◽  
Lipid Binding ◽  
And Function ◽  
Lipid Binding Proteins

Saccharomyces cerevisiae remains an ideal organism for studying the cell biological roles of lipids in vivo, as yeast has phospholipid metabolic pathways similar to mammalian cells, is easy and economical to manipulate, and is genetically tractable. The availability of isogenic strains containing specific genetic inactivation of each non-essential gene allowed for the development of a high-throughput method, called synthetic genetic analysis (SGA), to identify and describe precise pathways or functions associated with specific genes. This review describes the use of SGA to aid in elucidating the function of two lipid-binding proteins that regulate vesicular transport, Sec14 and Kes1. Sec14 was first identified as a phosphatidylcholine (PC) – phosphatidylinositol (PI) transfer protein required for viability, with reduced Sec14 function resulting in diminished vesicular transport out of the trans-Golgi. Although Sec14 is required for cell viability, inactivating the KES1 gene that encodes for a member of the oxysterol binding protein family in cells lacking Sec14 function results in restoration of vesicular transport and cell growth. SGA analysis identified a role for Kes1 and Sec14 in regulating the level and function of Golgi PI-4-phosphate (PI-4-P). SGA also determined that Sec14 not only regulates vesicular transport out of the trans-Golgi, but also transport from endosomes to the trans-Golgi. Comparing SGA screens in databases, coupled with genetic and cell biological analyses, further determined that the PI-4-P pool affected by Kes1 is generated by the PI 4-kinase Pik1. An important biological role for Sec14 and Kes1 revealed by SGA is coordinate regulation of the Pik1-generated Golgi PI-4-P pool that in turn is essential for vesicular transport into and out of the trans-Golgi.

scholarly journals Vertical silicon nanowires as a universal platform for delivering biomolecules into living cells

2010 ◽  
Vol 107 (5) ◽  
pp. 1870-1875 ◽  
Author(s):  
Alex K. Shalek ◽  
Jacob T. Robinson ◽  
Ethan S. Karp ◽  
Jin Seok Lee ◽  
Dae-Ro Ahn ◽  
...  
Keyword(s):  
Small Molecules ◽  
High Throughput ◽  
Silicon Nanowires ◽  
Mammalian Cells ◽  
Living Cells ◽  
Diverse Range ◽  
Viral Packaging ◽  
Neuronal Progenitor ◽  
Efficient Delivery ◽  
Surface Modified

A generalized platform for introducing a diverse range of biomolecules into living cells in high-throughput could transform how complex cellular processes are probed and analyzed. Here, we demonstrate spatially localized, efficient, and universal delivery of biomolecules into immortalized and primary mammalian cells using surface-modified vertical silicon nanowires. The method relies on the ability of the silicon nanowires to penetrate a cell’s membrane and subsequently release surface-bound molecules directly into the cell’s cytosol, thus allowing highly efficient delivery of biomolecules without chemical modification or viral packaging. This modality enables one to assess the phenotypic consequences of introducing a broad range of biological effectors (DNAs, RNAs, peptides, proteins, and small molecules) into almost any cell type. We show that this platform can be used to guide neuronal progenitor growth with small molecules, knock down transcript levels by delivering siRNAs, inhibit apoptosis using peptides, and introduce targeted proteins to specific organelles. We further demonstrate codelivery of siRNAs and proteins on a single substrate in a microarray format, highlighting this technology’s potential as a robust, monolithic platform for high-throughput, miniaturized bioassays.

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