Alkaline phosphatase reaction buffer (APRB)

2009 ◽  
Vol 2009 (8) ◽  
pp. pdb.rec11874-pdb.rec11874
Keyword(s):  
Alkaline Phosphatase ◽  
Alkaline Phosphatase Reaction

scholarly journals A Model of the Transition State in the Alkaline Phosphatase Reaction

1999 ◽  
Vol 274 (13) ◽  
pp. 8351-8354 ◽  
Author(s):  
Kathleen M. Holtz ◽  
Boguslaw Stec ◽  
Evan R. Kantrowitz
Keyword(s):  
Alkaline Phosphatase ◽  
Transition State ◽  
Alkaline Phosphatase Reaction

A study on the structure of the whiting integument (Merlangius merlangus L.)

1976 ◽  
Vol 56 (1) ◽  
pp. 213-226 ◽  
Author(s):  
Alistair M. Bullock ◽  
Ronald J. Roberts ◽  
John D. M. Gordon
Keyword(s):  
Alkaline Phosphatase ◽  
Mucous Cell ◽  
Succinic Dehydrogenase ◽  
The Body ◽  
Adult Fish ◽  
Succinic Dehydrogenase Activity ◽  
Acid Component ◽  
Merlangius Merlangus ◽  
Mature Fish ◽  
Alkaline Phosphatase Reaction

The histology and histochemistry of whiting skin was studied in larval, juvenile and adult fish. The adult specimens were obtained throughout the year but there was no evidence of seasonal variation in the areas of the body sampled. The skin of the whiting is considerably more complex than that of any other teleost fish which has been described in detail. The epidermis of the mature fish comprises five layers, consisting of Malpighian cells and a variety of other structures including large cystic bullae, which ultrastructurally and histochemically are relatively amorphous but comprise a major proportion of the epidermis. A reticulum of epidermal melanocytes occurs above these structures and two types of mucous cell are described. The major histochemical feature of the epidermis was the alkaline phosphatase reaction, similar to that found in higher animals. Succinic dehydrogenase activity was found at all levels. The epithelial mucins were mainly acid mucopolysaccharide with a small sialic acid component and a greater proportion of sulphated muco-substance. The dermis was morphologically similar to that of other scaled fishes. Mast cells were particularly prominent and occasional non-pigmented ‘melanophores’ were seen.

THE INFLUENCE OF OVARIAN HORMONES ON THE DECIDUAL ALKALINE PHOSPHATASE REACTION IN ADRENALECTOMIZED PSEUDOPREGNANT RATS

1971 ◽  
Vol 50 (3) ◽  
pp. 451-455
Author(s):  
J. P. MANNING
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Activity ◽  
Enzyme Reaction ◽  
Ovarian Hormones ◽  
Adrenal Hormone ◽  
Alkaline Phosphatase Reaction ◽  
Adrenalectomized Rats

SUMMARY The influence of oestradiol-17β and/or progesterone on decidual alkaline phosphatase was studied in adrenalectomized (day 1) and ovariectomized (day 1) rats after uterine traumatization on day 4 of pseudopregnancy. Enzyme activity was 10–15 times greater on day 8 of pseudopregnancy in untreated adrenalectomized and intact animals than in non-decidualized uteri. Treatment of ovariectomized and/or adrenalectomized rats with seven daily injections of 5 mg progesterone alone or combined with oestradiol-17β (0·1 μg on days 1–3, and 1·0 μg on days 4–7), did not alter the enzyme reaction. If 1·0 μg of the oestrogen was administered with 5 mg progesterone from days 1–7, no deciduoma response was observed. The results substantiate the hypothesis that progesterone is the only ovarian and adrenal hormone necessary for decidualization of traumatized uteri in pseudopregnant rats.

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