Selection medium for T-DNA insertion

2009 ◽  
Vol 2009 (2) ◽  
pp. pdb.rec11662-pdb.rec11662
Keyword(s):  
Dna Insertion ◽  
Selection Medium

scholarly journals mRNA Surveillance Complex PELOTA-HBS1 Regulates Phosphoinositide-Dependent Protein Kinase1 and Plant Growth

2021 ◽  
Author(s):  
Wei Kong ◽  
Shutang Tan ◽  
Qing Zhao ◽  
De-Li Lin ◽  
Zhi-Hong Xu ◽  
...  
Keyword(s):  
Quality Control ◽  
Messenger Rna ◽  
Loss Of Function ◽  
Yeast Saccharomyces Cerevisiae ◽  
Developmental Defects ◽  
Mrna Surveillance ◽  
Dna Insertion ◽  
Dependent Protein ◽  
Heterodimeric Complex ◽  
Severe Growth

Abstract The quality control system for messenger RNA (mRNA) is fundamental for cellular activities in eukaryotes. To elucidate the molecular mechanism of 3’-Phosphoinositide-Dependent Protein Kinase1 (PDK1), a master regulator that is essential throughout eukaryotic growth and development, we employed a forward genetic approach to screen for suppressors of the loss-of-function T-DNA insertion double mutant pdk1.1 pdk1.2 in Arabidopsis thaliana. Notably, the severe growth attenuation of pdk1.1 pdk1.2 was rescued by sop21 (suppressor of pdk1.1 pdk1.2), which harbours a loss-of-function mutation in PELOTA1 (PEL1). PEL1 is a homologue of mammalian PELOTA and yeast (Saccharomyces cerevisiae) DOM34p, which each form a heterodimeric complex with the GTPase HBS1 (HSP70 SUBFAMILY B SUPPRESSOR1, also called SUPERKILLER PROTEIN7, SKI7), a protein that is responsible for ribosomal rescue and thereby assures the quality and fidelity of mRNA molecules during translation. Genetic analysis further revealed that a dysfunctional PEL1-HBS1 complex failed to degrade the T-DNA-disrupted PDK1 transcripts, which were truncated but functional, and thus rescued the growth and developmental defects of pdk1.1 pdk1.2. Our studies demonstrated the functionality of a homologous PELOTA-HBS1 complex and identified its essential regulatory role in plants, providing insights into the mechanism of mRNA quality control.

scholarly journals Identification of highly transformable wheat genotypes for mass production of fertile transgenic plants

Genome ◽  
2002 ◽  
Vol 45 (2) ◽  
pp. 421-430 ◽  
Author(s):  
A Pellegrineschi ◽  
L M Noguera ◽  
B Skovmand ◽  
R M Brito ◽  
L Velazquez ◽  
...  
Keyword(s):  
Transformation Efficiency ◽  
Transformation Process ◽  
Plant Genotype ◽  
Donor Plant ◽  
Genotype Effect ◽  
Factors Influencing ◽  
Number Of Factors ◽  
Selection Medium ◽  
Callus Regeneration ◽  
Transformation Systems

The efficiency of wheat biolistic transformation systems strongly depends on the bombardment parameters, the condition of the donor plant, and the plant genotype chosen for the transformation process. This paper analyzes the transformation efficiency of the 129 wheat sister lines generically called 'Bobwhite', originally obtained from the cross 'Aurora'//'Kalyan'/'Bluebird 3'/'Woodpecker'. A number of factors influencing the transformation were examined, such as the ability to produce embryogenic callus, regeneration in selection medium, and overall transformation performance. Of the 129 genotypes evaluated, eight demonstrated transformation efficiencies above 60% (60 independent transgenic events per 100 immature embryos bombarded). Among the eight genotypes identified, we studied agronomic characteristics such as earliness to identify the most adaptable line(s) for different lab conditions. 'Bobwhite' SH 98 26 was identified as a super-transformable wheat line.Key words: wheat transformation, 'Bobwhite', genotype effect.

scholarly journals The Zebrafish Insertion Collection (ZInC): a web based, searchable collection of zebrafish mutations generated by DNA insertion

2012 ◽  
Vol 41 (D1) ◽  
pp. D861-D864 ◽  
Author(s):  
Gaurav K. Varshney ◽  
Haigen Huang ◽  
Suiyuan Zhang ◽  
Jing Lu ◽  
Derek E. Gildea ◽  
...  
Keyword(s):  
Web Based ◽  
Dna Insertion

DNA Insertion Elements, Plasmids and Episomes

FEBS Letters ◽  
1978 ◽  
Vol 93 (2) ◽  
pp. 380-381
Author(s):  
P. Broda
Keyword(s):  
Insertion Elements ◽  
Dna Insertion
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