Neurobasal medium (PSG)

2008 ◽  
Vol 2008 (12) ◽  
pp. pdb.rec11501-pdb.rec11501
Keyword(s):  
Neurobasal Medium

Survival of hippocampal and cortical neurons in a mixture of MEM+ and B27-supplemented neurobasal medium

2000 ◽  
Vol 28 (5) ◽  
pp. 665-672 ◽  
Author(s):  
Chengsong Xie ◽  
William R Markesbery ◽  
Mark A Lovell
Keyword(s):  
Cortical Neurons ◽  
Neurobasal Medium

scholarly journals Comparison of Rat Primary Midbrain Neurons Cultured in DMEM/F12 and Neurobasal Mediums

2021 ◽  
Vol 12 (2) ◽  
pp. 205-212
Author(s):  
Neda Valian ◽  
◽  
Mansooreh Heravi ◽  
Abolhassan Ahmadiani ◽  
Leila Dargahi ◽  
...  
Keyword(s):  
Light Microscopy ◽  
Dopaminergic Neurons ◽  
Motor Behavior ◽  
Cell Number ◽  
High Price ◽  
Rat Embryo ◽  
Brain Functions ◽  
Midbrain Neurons ◽  
Neurobasal Medium ◽  
Learning And Cognition

Introduction: Midbrain dopaminergic neurons are involved in various brain functions, including motor behavior, reinforcement, motivation, learning, and cognition. Primary dopaminergic neurons and also several lines of these cells are extensively used in cell culture studies. Primary dopaminergic neurons prepared from rodents have been cultured in both DMEM/F12 and neurobasal mediums in several studies. However, there is no document reporting the comparison of these two mediums. So in this study, we evaluated the neurons and astroglial cells in primary midbrain neurons from rat embryos cultured in DMEM/F12 and neurobasal mediums. Methods: Primary mesencephalon cells were prepared from the E14.5 rat embryo. Then they were seeded in two different mediums ( Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 [DMEM/F12] and neurobasal). On day 3 and day 5, half of the medium was replaced with a fresh medium. On day 7, β3-tubulin-, GFAP (Glial fibrillary acidic protein)- and Tyrosine Hydroxylase TH-positive cells were characterized as neurons, astrocytes, and dopaminergic neurons, respectively, using immunohistochemistry. Furthermore, the morphology of the cells in both mediums was observed under light microscopy on days 1, 3, and 5. Results: The cells cultured in both mediums were similar under light microscopy regarding the cell number, but in a neurobasal medium, the cells have aggregated and formed clustering structures. Although GFAP-immunoreactive cells were lower in neurobasal compared to DMEM/F12, the number of β3-tubulin- and TH-positive cells in both cultures was the same. Conclusion: This study’s findings demonstrated that primary midbrain cells from the E14.5 rat embryo could grow in both DMEM/F12 and neurobasal mediums. Therefore, considering the high price of a neurobasal medium, it can be replaced with DMEM/F12 for culturing primary dopaminergic neurons.

Neurobasal medium toxicity on mature cortical neurons

Neuroreport ◽  
2015 ◽  
Vol 26 (6) ◽  
pp. 320-324 ◽  
Author(s):  
Daniele Maggioni ◽  
Marianna Monfrini ◽  
Maddalena Ravasi ◽  
Giovanni Tredici ◽  
Arianna Scuteri
Keyword(s):  
Cortical Neurons ◽  
Neurobasal Medium ◽  
Medium Toxicity
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