NBT staining solution

2007 ◽  
Vol 2007 (8) ◽  
pp. pdb.rec11024-pdb.rec11024
Keyword(s):  
Staining Solution ◽  
Nbt Staining

scholarly journals Fourier transform infrared spectroscopy coupled with machine learning classification for identification of oxidative damage in freeze-dried heart valves

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Dejia Liu ◽  
Sükrü Caliskan ◽  
Bita Rashidfarokhi ◽  
Harriëtte Oldenhof ◽  
Klaus Jung ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Oxidative Damage ◽  
Fourier Transform Infrared Spectroscopy ◽  
Heart Valve ◽  
Freeze Drying ◽  
Heart Valves ◽  
Fourier Transform Infrared ◽  
Freeze Dried ◽  
Nbt Staining

AbstractFreeze-drying can be used to ensure off-the-shelf availability of decellularized heart valves for cardiovascular surgery. In this study, decellularized porcine aortic heart valves were analyzed by nitroblue tetrazolium (NBT) staining and Fourier transform infrared spectroscopy (FTIR) to identify oxidative damage during freeze-drying and subsequent storage as well as after treatment with H2O2 and FeCl3. NBT staining revealed that sucrose at a concentration of at least 40% (w/v) is needed to prevent oxidative damage during freeze-drying. Dried specimens that were stored at 4 °C depict little to no oxidative damage during storage for up to 2 months. FTIR analysis shows that fresh control, freeze-dried and stored heart valve specimens cannot be distinguished from one another, whereas H2O2- and FeCl3-treated samples could be distinguished in some tissue section. A feed forward artificial neural network model could accurately classify H2O2 and FeCl3 treated samples. However, fresh control, freeze-dried and stored samples could not be distinguished from one another, which implies that these groups are very similar in terms of their biomolecular fingerprints. Taken together, we conclude that sucrose can minimize oxidative damage caused by freeze-drying, and that subsequent dried storage has little effects on the overall biochemical composition of heart valve scaffolds.

Staining solution A

2008 ◽  
Vol 2008 (8) ◽  
pp. pdb.rec11401-pdb.rec11401
Keyword(s):  
Staining Solution

Hoechst staining solution

2007 ◽  
Vol 2007 (23) ◽  
pp. pdb.rec10914-pdb.rec10914
Keyword(s):  
Staining Solution ◽  
Hoechst Staining

Staining solution (SS)

2009 ◽  
Vol 2009 (5) ◽  
pp. pdb.rec11789-pdb.rec11789
Keyword(s):  
Staining Solution

scholarly journals A new technique for staining sections and recovering celloidin film in high resolution autoradiography.

1983 ◽  
Vol 31 (7) ◽  
pp. 964-966
Author(s):  
G Pradal ◽  
P Barriere ◽  
F Mollat ◽  
G Lefranc
Keyword(s):  
Heavy Metals ◽  
Stainless Steel ◽  
High Resolution ◽  
Filter Paper ◽  
New Technique ◽  
Staining Solution ◽  
High Resolution Autoradiography ◽  
Ultrathin Sections ◽  
A New Technique

Ultrathin sections are stained immediately after cutting by placing them in contact with staining solution and then placed on a slide covered by a celloidin film. This method largely avoids precipitates of heavy metals. The recovering of celloidin film is improved using a stainless steel basket. This technique is far more reliable than that involving use of a filter paper.

scholarly journals Discoloration of Coating Resins Exposed to Staining Solution

2012 ◽  
Vol 32 (2) ◽  
pp. 74-80
Author(s):  
Mai YAMAGUCHI ◽  
Hisashi HISAMITSU ◽  
Atsufumi MANABE
Keyword(s):  
Staining Solution
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