Resuspension Buffer for Plant Ribosomes

2015 ◽  
Vol 2015 (3) ◽  
pp. pdb.rec081612
Keyword(s):  
Plant Ribosomes

scholarly journals Effect of RIP Overexpression on Abiotic Stress Tolerance and Development of Rice

2021 ◽  
Vol 22 (3) ◽  
pp. 1434
Author(s):  
Pieter Wytynck ◽  
Jeroen Lambin ◽  
Simin Chen ◽  
Sinem Demirel Asci ◽  
Isabel Verbeke ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Stress Responses ◽  
Abiotic Stress Tolerance ◽  
Protein Translation ◽  
Ribosome Inactivating Proteins ◽  
Close Proximity ◽  
Inhibit Protein Translation ◽  
Plant Ribosomes ◽  
A Cell ◽  
Type 3

Ribosome-inactivating proteins (RIPs) are a class of cytotoxic enzymes that can inhibit protein translation by depurinating rRNA. Most plant RIPs are synthesized with a leader sequence that sequesters the proteins to a cell compartment away from the host ribosomes. However, several rice RIPs lack these signal peptides suggesting they reside in the cytosol in close proximity to the plant ribosomes. This paper aims to elucidate the physiological function of two nucleocytoplasmic RIPs from rice, in particular, the type 1 RIP referred to as OsRIP1 and a presumed type 3 RIP called nuRIP. Transgenic rice lines overexpressing these RIPs were constructed and studied for developmental effects resulting from this overexpression under greenhouse conditions. In addition, the performance of transgenic seedlings in response to drought, salt, abscisic acid and methyl jasmonate treatment was investigated. Results suggest that both RIPs can affect methyl jasmonate mediated stress responses.

Functions of cytokinins

1978 ◽  
Vol 284 (1002) ◽  
pp. 449-457 ◽  
Keyword(s):  
Cell Division ◽  
Binding Protein ◽  
Leaf Tissue ◽  
Mechanisms Of Action ◽  
Transfer Rna ◽  
Biological Functions ◽  
Higher Plant ◽  
Rna Molecules ◽  
Plant Ribosomes ◽  
Stimulation Of

Mechanisms of action of cytokinins at the cellular and molecular levels are still unknown. Biological functions of cytokinins are presented through specific bioassay systems which are regarded as standard (delay of senescence of leaf tissue and stimulation of cell division) and which have been or may be biochemically investigated. These ‘biochemical functions’ of cytokinins are reviewed. The biochemical significance of the specific occurrence of cytokinins in transfer RNA molecules is discussed with respect to the question of the incorporation of labelled cytokinins into RNA molecules. Also, the significance of the cytokinin binding protein recently isolated from higher plant ribosomes is discussed.

Über die Ribosomen aus Blättern von Allium porrum

1962 ◽  
Vol 17 (12) ◽  
pp. 837-847 ◽  
Author(s):  
Ruth Ehring
Keyword(s):  
Ribosomal Protein ◽  
Ribonucleic Acid ◽  
Dry Weight ◽  
Allium Porrum ◽  
Structural Protein ◽  
Amino Terminal ◽  
Plant Ribosomes ◽  
Detergent Treatment ◽  
Phenol Treatment ◽  
Protein Moiety

Plant ribosomes prepared from growing leaves of Allium porrum were purified by differential centrifugation. Isolated ribosomes had a sedimentation constant of 79 —80 S and a ribonucleic acid content of at least 34% dry weight. The ribonucleic acid could be separated from the protein moiety by detergent treatment and was found to sediment as three fractions with sedimentation constants of 27 S, 9-10 S and 5 —6 S respectively. Similar ribonucleic acid components were obtained by phenol treatment of unfractionated leaf homogenates.A comparison of the nucleotide and amino acid composition of Allium porrum ribosomes with data reported for ribosomes from other plant and animal tissues revealed no significant differences. However, the lysine and arginine content of Allium porrum ribosomal protein was found to be significantly greater than that reported for the structural protein of the chloroplasts of the same species.Analysis of the amino terminal end groups of the Allium porrum ribosomal protein yielded predominantly serine.

Salt-tolerance in plants. I. Ions, compatible organic solutes and the stability of plant ribosomes

1984 ◽  
Vol 7 (8) ◽  
pp. 571-578
Author(s):  
C. J. BRADY ◽  
T. S. GIBSON ◽  
E. W. R. BARLOW ◽  
J. SPEIRS ◽  
R. G. WYN JONES
Keyword(s):  
Salt Tolerance ◽  
Organic Solutes ◽  
Plant Ribosomes ◽  
The Stability

Region 1112–1123 in the central domain of 18S rRNA in 40S subunits of plant ribosomes: Accessibility for complementary interactions and the functional role

2010 ◽  
Vol 36 (3) ◽  
pp. 336-343 ◽  
Author(s):  
A. V. Zhigailov ◽  
D. M. Graifer ◽  
E. S. Babaylova ◽  
N. S. Polimbetova ◽  
G. G. Karpova ◽  
...  
Keyword(s):  
Functional Role ◽  
18S Rrna ◽  
Central Domain ◽  
Plant Ribosomes ◽  
Complementary Interactions

scholarly journals Differential cytokinin binding to dioecious plant ribosomes

FEBS Letters ◽  
1979 ◽  
Vol 102 (2) ◽  
pp. 211-215 ◽  
Author(s):  
S.R. Chung ◽  
R. Durand ◽  
B. Durand
Keyword(s):  
Dioecious Plant ◽  
Plant Ribosomes
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