scholarly journals Time-Lapse Imaging of Postimplantation Mouse Embryos

2011 ◽  
Vol 2011 (4) ◽  
pp. pdb.prot5595-pdb.prot5595 ◽  
Author(s):  
M. D. Garcia ◽  
R. S. Udan ◽  
A.-K. Hadjantonakis ◽  
M. E. Dickinson
Keyword(s):  
Time Lapse ◽  
Mouse Embryos ◽  
Time Lapse Imaging

scholarly journals Microwells support high-resolution time-lapse imaging and development of preimplanted mouse embryos

2015 ◽  
Vol 9 (2) ◽  
pp. 022407 ◽  
Author(s):  
Yu-Hsiang Chung ◽  
Yi-Hsing Hsiao ◽  
Wei-Lun Kao ◽  
Chia-Hsien Hsu ◽  
Da-Jeng Yao ◽  
...  
Keyword(s):  
High Resolution ◽  
Time Lapse ◽  
Mouse Embryos ◽  
Resolution Time ◽  
Time Lapse Imaging

scholarly journals Epithelial geometry regulates spindle orientation and progenitor fate during formation of the mammalian epidermis

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Kimberly Box ◽  
Bradley W Joyce ◽  
Danelle Devenport
Keyword(s):  
Cell Fate ◽  
Planar Cell Polarity ◽  
Time Lapse ◽  
Mouse Embryos ◽  
Spindle Orientation ◽  
Basal Cells ◽  
Tissue Architecture ◽  
Cell Geometry ◽  
Oriented Cell Division ◽  
Time Lapse Imaging

The control of cell fate through oriented cell division is imperative for proper organ development. Basal epidermal progenitor cells divide parallel or perpendicular to the basement membrane to self-renew or produce differentiated stratified layers, but the mechanisms regulating the choice between division orientations are unknown. Using time-lapse imaging to follow divisions and fates of basal progenitors, we find that mouse embryos defective for the planar cell polarity (PCP) gene, Vangl2, exhibit increased perpendicular divisions and hyperthickened epidermis. Surprisingly, this is not due to defective Vangl2 function in the epidermis, but to changes in cell geometry and packing that arise from the open neural tube characteristic of PCP mutants. Through regional variations in epidermal deformation and physical manipulations, we show that local tissue architecture, rather than cortical PCP cues, regulates the decision between symmetric and stratifying divisions, allowing flexibility for basal cells to adapt to the needs of the developing tissue.

Cleavage of Human Embryos: Options and Diversity

Acta Naturae ◽  
2016 ◽  
Vol 8 (3) ◽  
pp. 88-96
Author(s):  
Yu. K. Doronin ◽  
I. V. Senechkin ◽  
L. V. Hilkevich ◽  
M. A. Kurcer
Keyword(s):  
Time Lapse ◽  
Reproductive Technologies ◽  
Human Embryos ◽  
Imaging Data ◽  
Noninvasive Methods ◽  
Topographic Features ◽  
Time Parameters ◽  
Embryo Cleavage ◽  
Time Lapse Imaging ◽  
Developmental Dynamics

In order to estimate the diversity of embryo cleavage relatives to embryo progress (blastocyst formation), time-lapse imaging data of preimplantation human embryo development were used. This retrospective study is focused on the topographic features and time parameters of the cleavages, with particular emphasis on the lengths of cleavage cycles and the genealogy of blastomeres in 2- to 8-cell human embryos. We have found that all 4-cell human embryos have four developmental variants that are based on the sequence of appearance and orientation of cleavage planes during embryo cleavage from 2 to 4 blastomeres. Each variant of cleavage shows a strong correlation with further developmental dynamics of the embryos (different cleavage cycle characteristics as well as lengths of blastomere cycles). An analysis of the sequence of human blastomere divisions allowed us to postulate that the effects of zygotic determinants are eliminated as a result of cleavage, and that, thereafter, blastomeres acquire the ability of own syntheses, regulation, polarization, formation of functional contacts, and, finally, of specific differentiation. This data on the early development of human embryos obtained using noninvasive methods complements and extend our understanding of the embryogenesis of eutherian mammals and may be applied in the practice of reproductive technologies.

scholarly journals Evaluating the utility of time-lapse imaging in the estimation of post-mortem interval: An Australian case study

2019 ◽  
Vol 1 ◽  
pp. 204-210 ◽  
Author(s):  
Alyson Wilson ◽  
Stanley Serafin ◽  
Dilan Seckiner ◽  
Rachel Berry ◽  
Xanthé Mallett
Keyword(s):  
Time Lapse ◽  
Post Mortem ◽  
Post Mortem Interval ◽  
Australian Case ◽  
Time Lapse Imaging

scholarly journals Time-lapse imaging of CO2 migration within near-surface sediments during a controlled sub-seabed release experiment

2021 ◽  
Vol 109 ◽  
pp. 103363
Author(s):  
Ben Roche ◽  
Jonathan M. Bull ◽  
Hector Marin-Moreno ◽  
Timothy G. Leighton ◽  
Ismael H. Falcon-Suarez ◽  
...  
Keyword(s):  
Surface Sediments ◽  
Time Lapse ◽  
Near Surface ◽  
Release Experiment ◽  
Time Lapse Imaging

Segmentation and Track-Analysis in Time-Lapse Imaging of Bacteria

2016 ◽  
Vol 10 (1) ◽  
pp. 174-184 ◽  
Author(s):  
Sajith Kecheril Sadanandan ◽  
Ozden Baltekin ◽  
Klas E. G. Magnusson ◽  
Alexis Boucharin ◽  
Petter Ranefall ◽  
...  
Keyword(s):  
Time Lapse ◽  
Time Lapse Imaging ◽  
Track Analysis

scholarly journals Analysis of Ca2+ response of osteocyte network by three-dimensional time-lapse imaging in living bone

2017 ◽  
Vol 36 (5) ◽  
pp. 519-528 ◽  
Author(s):  
Tomoyo Tanaka ◽  
Mitsuhiro Hoshijima ◽  
Junko Sunaga ◽  
Takashi Nishida ◽  
Mana Hashimoto ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Time Lapse ◽  
Living Bone ◽  
Time Lapse Imaging
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