Voltage-Clamp Analysis of Synaptic Transmission at the Drosophila Larval Neuromuscular Junction

2010 ◽  
Vol 2010 (9) ◽  
pp. pdb.prot5488-pdb.prot5488 ◽  
Author(s):  
B. Zhang ◽  
B. Stewart
Keyword(s):  
Neuromuscular Junction ◽  
Synaptic Transmission ◽  
Voltage Clamp ◽  
Voltage Clamp Analysis ◽  
Larval Neuromuscular Junction

A cGMP-dependent protein kinase (PKG) controls synaptic transmission tolerance to acute oxidative stress at the Drosophila larval neuromuscular junction

2013 ◽  
Vol 109 (3) ◽  
pp. 649-658 ◽  
Author(s):  
Stacee Lee Caplan ◽  
Sarah L. Milton ◽  
Ken Dawson-Scully
Keyword(s):  
Oxidative Stress ◽  
Protein Kinase ◽  
Neuromuscular Junction ◽  
Synaptic Transmission ◽  
Fruit Fly ◽  
Synaptic Function ◽  
Dependent Protein Kinase ◽  
Cgmp Dependent Protein Kinase ◽  
Dependent Protein ◽  
Larval Neuromuscular Junction

Increasing evidence demonstrates that modulating the cGMP-dependent protein kinase G (PKG) pathway produces an array of behavioral phenotypes in the fruit fly, Drosophila melanogaster. Altering PKG activity, either genetically via the foraging ( for) gene or using pharmacology modifies tolerance to acute abiotic stresses such as hyperthermia and hypoxia. PKG signaling has been shown to modulate neuroprotection in many experimental paradigms of acute brain trauma and chronic neurodegenerative diseases. However, relatively little is known about how this stress-induced neuroprotective mechanism affects neural communication. In this study, we investigated the role PKG activity has on synaptic transmission at the Drosophila larval neuromuscular junction (NMJ) during acute oxidative stress and found that the application of 2.25 mM hydrogen peroxide (H2O2) disrupts synaptic function by rapidly increasing the rate of neuronal failure. Here, we report that reducing PKG activity through either natural genetic variation or an induced mutation of the for gene increases synaptic tolerance during acute oxidative conditions. Furthermore, pharmacological manipulations revealed that neurotransmission is significantly extended during acute H2O2 exposure upon inhibition of the PKG pathway. Conversely, activation of this signaling cascade using either genetics or pharmacology significantly reduced the time until synaptic failure. Therefore, these findings suggest a potential role for PKG activity to regulate the tolerance of synaptic transmission during acute oxidative stress, where inhibition promotes functional protection while activation increases susceptibility to neurotransmission breakdown.

Voltage-clamp analysis of nodes of Ranvier in regenerated rat sciatic nerve

1987 ◽  
Vol 409 (2) ◽  
pp. 227-235 ◽  
Author(s):  
T. Brismar ◽  
C. Hildebrand ◽  
S. Berglund
Keyword(s):  
Sciatic Nerve ◽  
Voltage Clamp ◽  
Nodes Of Ranvier ◽  
Rat Sciatic Nerve ◽  
Voltage Clamp Analysis

scholarly journals Input-Specific Plasticity and Homeostasis at the Drosophila Larval Neuromuscular Junction

Neuron ◽  
2017 ◽  
Vol 93 (6) ◽  
pp. 1388-1404.e10 ◽  
Author(s):  
Zachary L. Newman ◽  
Adam Hoagland ◽  
Krishan Aghi ◽  
Kurtresha Worden ◽  
Sabrina L. Levy ◽  
...  
Keyword(s):  
Neuromuscular Junction ◽  
Larval Neuromuscular Junction
Sign in / Sign up

Export Citation Format

Share Document