Overexpressing Tagged Proteins in Plants Using a Modified Gateway Cloning Strategy

2010 ◽  
Vol 2010 (3) ◽  
pp. pdb.prot5401-pdb.prot5401 ◽  
Author(s):  
M. J. Dubin ◽  
C. Bowler ◽  
G. Benvenuto
Keyword(s):  
Gateway Cloning ◽  
Cloning Strategy

A Modified MultiSite Gateway Cloning Strategy for Consolidation of Genes in Plants

2012 ◽  
Vol 53 (2) ◽  
pp. 129-138 ◽  
Author(s):  
Ramu S. Vemanna ◽  
Babitha K. Chandrashekar ◽  
H. M. Hanumantha Rao ◽  
Shailesh K. Sathyanarayanagupta ◽  
K. S. Sarangi ◽  
...  
Keyword(s):  
Gateway Cloning ◽  
Cloning Strategy

scholarly journals A modified Gateway cloning strategy for overexpressing tagged proteins in plants

Plant Methods ◽  
2008 ◽  
Vol 4 (1) ◽  
pp. 3 ◽  
Author(s):  
Manu J Dubin ◽  
Chris Bowler ◽  
Giovanna Benvenuto
Keyword(s):  
Gateway Cloning ◽  
Cloning Strategy

Research Progress on Plant Expression Vectors for Gateway Cloning Technology

2012 ◽  
Vol 30 (5) ◽  
pp. 528
Author(s):  
Su-Qin XIAO ◽  
Zhen SUN ◽  
Xiu-Xia XUAN ◽  
Li-Mei CHEN
Keyword(s):  
Research Progress ◽  
Expression Vectors ◽  
Gateway Cloning ◽  
Plant Expression ◽  
Cloning Technology

A simplified cloning strategy for the generation of an endothelial cell selective recombinant adenovirus vector

2006 ◽  
Vol 135 (1) ◽  
pp. 127-135 ◽  
Author(s):  
Chitladda Mahanivong ◽  
Jörg A. Krüger ◽  
Dafang Bian ◽  
Ralph A. Reisfeld ◽  
Shuang Huang
Keyword(s):  
Endothelial Cell ◽  
Recombinant Adenovirus ◽  
Adenovirus Vector ◽  
Cloning Strategy ◽  
Recombinant Adenovirus Vector

scholarly journals Gateway cloning vectors for the LexA-based binary expression system in Drosophila

Fly ◽  
2008 ◽  
Vol 2 (4) ◽  
pp. 236-239 ◽  
Author(s):  
Soeren Diegelmann ◽  
Michael Bate ◽  
Matthais Landgraf
Keyword(s):  
Expression System ◽  
Cloning Vectors ◽  
Gateway Cloning

scholarly journals A cloning strategy to obtain recombinant proteins with identical primary structure to the native forms

2010 ◽  
Vol 149 (1-2) ◽  
pp. 21-23
Author(s):  
Margarida Santana ◽  
Maria Carmen Portillo ◽  
Juan M. Gonzalez
Keyword(s):  
Recombinant Proteins ◽  
Primary Structure ◽  
Cloning Strategy

scholarly journals Mega primer-mediated molecular cloning strategy for chimaeragenesis and long DNA fragment insertion

2017 ◽  
Vol 37 (2) ◽  
Author(s):  
Hui Zhang ◽  
Chang-Jun Liu ◽  
Hui Jiang ◽  
Lu Zhou ◽  
Wen-Ying Li ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
High Efficiency ◽  
Low Cost ◽  
Plasmid Vector ◽  
Efficient Tool ◽  
Overlap Extension ◽  
Cloning Strategy ◽  
Dna Fragment ◽  
Fragment Insertion ◽  
Dpni Digestion

Molecular cloning methods based on primer and overlap-extension PCR are widely used due to their simplicity, reliability, low cost and high efficiency. In this article, an efficient mega primer-mediated (MP) cloning strategy for chimaeragenesis and long DNA fragment insertion is presented. MP cloning is a seamless, restriction/ligation-independent method that requires only three steps: (i) the first PCR for mega primer generation; (ii) the second PCR for exponential amplification mediated by the mega primers and (iii) DpnI digestion and transformation. Most importantly, for chimaeragenesis, genes can be assembled and constructed into the plasmid vector in a single PCR step. By employing this strategy, we successfully inserted four DNA fragments (approximately 500 bp each) into the same vector simultaneously. In conclusion, the strategy proved to be a simple and efficient tool for seamless cloning.

scholarly journals A novel cloning strategy for isolating, genotyping and phenotyping genetic variants of geminiviruses

2008 ◽  
Vol 5 (1) ◽  
pp. 135 ◽  
Author(s):  
Cica Urbino ◽  
Gael Thébaud ◽  
Martine Granier ◽  
Stéphane Blanc ◽  
Michel Peterschmitt
Keyword(s):  
Genetic Variants ◽  
Cloning Strategy
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