DNA Microinjection into the Macronucleus of Paramecium

2010 ◽  
Vol 2010 (1) ◽  
pp. pdb.prot5364-pdb.prot5364 ◽  
Author(s):  
J. Beisson ◽  
M. Betermier ◽  
M.-H. Bre ◽  
J. Cohen ◽  
S. Duharcourt ◽  
...  
Keyword(s):  
Dna Microinjection

Effect of GnRH injection timing in the production of pronuclear-stage zygotes used for DNA microinjection

Zygote ◽  
2004 ◽  
Vol 12 (3) ◽  
pp. 257-261 ◽  
Author(s):  
Hernan Baldassarre ◽  
Bin Wang ◽  
Melanie Gauthier ◽  
Nathalie Neveu ◽  
Anthoula Lazaris ◽  
...  
Keyword(s):  
Medroxyprogesterone Acetate ◽  
Treatment Protocol ◽  
Hormonal Treatment ◽  
Injection Timing ◽  
Chi Square ◽  
Cell Stage ◽  
Embryo Recovery ◽  
Stage Of Development ◽  
Production Programme ◽  
Dna Microinjection

This study was aimed at developing a hormonal treatment protocol in order to optimize the proportion of pronuclear-stage embryos to be used for DNA microinjection in a goat transgenic founder production programme. A total of 46 adult BELE® and 47 adult standard goats (1–5 years old) were used as donors and recipients, respectively. They were heat-synchronized using intravaginal sponges containing 60 mg medroxyprogesterone acetate for 10 days with an injection of 125 μg cloprostenol on the morning of the eighth day. Recipients were injected with 400 IU eCG at the time of sponge removal while donors received a total of 133 mg NIH-FSH-P1 (Folltropin-V) given twice daily in decreasing doses over 3 days starting 48 h before sponge removal. Ovulation was induced in donors by injecting 100 μg of GnRH at 24 h (GnRH24) or 36 h (GnRH36) after sponge removal. Embryo recovery was performed by oviduct flushing following a standard mid-ventral laparotomy procedure. The proportion of embryos in the pronuclear stage of development was higher in the GnRH36 group (90% vs 34%, p<0.01). Embryos were microinjected with a DNA expression cassette followed by transfer to the oviduct of synchronized recipients. A higher, yet not statistically significant, pregnancy rate was found in the recipients transferred with pronuclear-stage embryos compared with those transferred with 2-cell-stage embryos (64% vs 37%, chi-square p=0.06). One transgenic female founder was produced from the group of recipients transferred with pronuclear-stage microinjected embryos.

Transgenic Animals Derived by DNA Microinjection

2014 ◽  
pp. 77-88
Author(s):  
Marianne Brüggemann ◽  
Michael J. Osborn ◽  
Biao Ma ◽  
Suzanne Avis ◽  
Ignacio Anegon ◽  
...  
Keyword(s):  
Transgenic Animals ◽  
Dna Microinjection

Transgenic Birds by DNA Microinjection

1994 ◽  
Vol 12 (1) ◽  
pp. 60-63 ◽  
Author(s):  
Jamie Love ◽  
Clare Gribbin ◽  
Christine Mather ◽  
Helen Sang
Keyword(s):  
Dna Microinjection

Birth of piglets following DNA microinjection of in vitro‐fertilized porcine zygotes

1998 ◽  
Vol 9 (3) ◽  
pp. 199-200
Author(s):  
Melissa A. Larson ◽  
Lalantha R. Abeydeera ◽  
Billy N. Day ◽  
H. Michael Kubisch
Keyword(s):  
Dna Microinjection

Production of Transgenic Ruminants by DNA Microinjection

2002 ◽  
pp. 337-357 ◽  
Author(s):  
H. Niemann ◽  
H.H. Dopke ◽  
K.G. Hadeler
Keyword(s):  
Dna Microinjection
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