Dye Loading with Patch Pipettes

2009 ◽  
Vol 2009 (4) ◽  
pp. pdb.prot5201-pdb.prot5201 ◽  
Author(s):  
J. Eilers ◽  
A. Konnerth
Keyword(s):  
2017 ◽  
Vol 5 (2) ◽  
pp. 80
Author(s):  
Anuradha Bera ◽  
Shatrughan Malav ◽  
Bajrang Lal Tiwari ◽  
Shyam Govind Vaijapurkar

Colourless polystyrene-leucomalachite green (PS-LMG) thick films containing a suitable chloroalkane were prepared by a fast and facile casting method, and were investigated for their radio chromic response behavior under the influence of 1.25 MeV γ-radiation. Their gamma response was studied in the 0.05 kGy to 10 kGy range to evaluate their suitability for potential use as the dosimeter in the radiation processing industries. The films were found to undergo a visibly distinct green coloration in the studied range, with the colour intensity increasing with an increase in the total dose. The radiochromic response of these films when investigated as a function of film thickness showed that the colour development as well as the linearity of the response was markedly affected by the thickness of the films. The effect of dye loading and the chloroalkane concentration on the radiochromic response of these films were also investigated. Depending upon the film thickness and reactant concentrations, the films were found to be capable of visually detecting gamma radiation doses as low as few tens of grays.


RSC Advances ◽  
2017 ◽  
Vol 7 (67) ◽  
pp. 42013-42023 ◽  
Author(s):  
Ting-Kuang Chang ◽  
Yun Chi

The sensitizer TF-tBu_C3F7 has shown the highest overall efficiencies of JSC = 18.47 mA cm−2, VOC = 767 mV, FF = 0.71 and PCE = 10.05% under simulated one sun irradiation, due to the fine balance between dye loading and reduced charge recombination.


2011 ◽  
Vol 62 (10) ◽  
pp. 1007-1015 ◽  
Author(s):  
Alagappan Annamalai ◽  
Yang Dam Eo ◽  
Chan Im ◽  
Man-Jong Lee

2019 ◽  
Vol 23 (04n05) ◽  
pp. 599-610 ◽  
Author(s):  
Siddhartha Kumar ◽  
Whitney Webre ◽  
Jacob Schaffner ◽  
Sheikh M. S. Islam ◽  
Francis D’Souza ◽  
...  

The first example of A2B2 tetrabenzoporphyrin (KW-4) was synthesized, characterized and evaluated as a sensitizer for dye-sensitized solar cells. UV-vis and fluorescence spectroscopy revealed red-shifted and broadened absorption spectra of A2B2 tetrabenzoporphyrin as compared with its A2 dibenzo- and A2B2 dibenzoporphyrin analogues, which is a desired feature of dyes for dye-sensitized solar cells. DFT calculations also indicate favorable electron density distribution on the HOMO and LUMO of KW-4. However, the power conversion efficiency of the solar cell based on tetrabenzoporphyrin KW-4 displayed inferior performance than that of the solar cell based on A2 dibenzoporphyrin KW-2. The lower performance of the KW-4 cell was ascribed to two factors: the low lying LUMO energy level leading to less efficient electron injection and the “flat geometry” of the dye on TiO2surface facilitating charge recombination and decreasing dye loading. The investigation of anchoring group effect suggests that the acrylic acid group is a better anchoring group than pentadienyl carboxylic acid.


2014 ◽  
Vol 16 (16) ◽  
pp. 7448-7454 ◽  
Author(s):  
P. S. Archana ◽  
Arunava Gupta ◽  
Mashitah M. Yusoff ◽  
Rajan Jose

Tungsten doping in TiO2 nanowires led to increased photocurrent density resulting from increased lifetime and dye-loading compared to niobium doping.


2015 ◽  
Vol 240 ◽  
pp. 48-60 ◽  
Author(s):  
Mohammad I.K. Hamad ◽  
Martin Krause ◽  
Petra Wahle

RSC Advances ◽  
2018 ◽  
Vol 8 (56) ◽  
pp. 31943-31949 ◽  
Author(s):  
Md Ataul Mamun ◽  
Qiquan Qiao ◽  
Brian A. Logue

Functionalized carboxylate deposition involves deposition of molecules from the gas phase and is an alternative dye loading technique to dip-coating. It was used to create a monolayer of large molecular weight dyes on TiO2, providing multiple advantages to dip-coating.


2001 ◽  
Vol 85 (2) ◽  
pp. 986-994 ◽  
Author(s):  
Yang Li ◽  
Lynne A. Holtzclaw ◽  
James T. Russell

We have measured agonist evoked Ca2+ waves in Müller cells in situ within freshly isolated retinal slices. Using an eye cup dye loading procedure we were able to preferentially fill Müller glial cells in retinal slices with calcium green. Fluorescence microscopy revealed that bath perfusion of slices with purinergic agonists elicits Ca2+ waves in Müller cells, which propagate along their processes. These Ca2+ signals were insensitive to tetrodotoxin (TTX, 1.0 μM) pretreatment. Cells were readily identified as Müller cells by their unique morphology and by subsequent immunocytochemical labeling with glial fibrillary acidic protein antibodies. While cells never exhibited spontaneous Ca2+ oscillations, purinoreceptor agonists, ATP, 2 MeSATP, ADP, 2 MeSADP, and adenosine readily elicited Ca2+ waves. These waves persisted in the absence of [Ca2+]o but were abolished by thapsigargin pretreatment, suggesting that the purinergic agonists tested act by releasing Ca2+ from intracellular Ca2+ stores. The rank order of potency of different purines and pyrimidines for inducing Ca2+ signals was 2 MeSATP = 2MeSADP > ADP > ATP ≫ αβmeATP = uridine triphosphate (UTP) > uridine diphosphate (UDP). The Ca2+signals evoked by ATP, ADP, and 2 MeSATP were inhibited by reactive blue (100 μM) and suramin (200 μM), and the adenosine induced signals were abolished only by 3,7-dimethyl-1-propargylxanthine (200 μM) and not by 1,3-dipropyl-8-(2-amino-4-chlorophenyl)-xanthine) or 8-cyclopentyl-1,3-dipropylxanthine at the same concentration. Based on these pharmacological characteristics and the dose-response relationships for ATP, 2 MeSATP, 2 MeSADP, ADP, and adenosine, we concluded that Müller cells express the P1A2 and P2Y1 subtypes of purinoceptors. Analysis of Ca2+ responses showed that, similar to glial cells in culture, wave propagation occurred by regenerative amplification at specialized Ca2+ release sites (wave amplification sites), where the rate of Ca2+ release was significantly enhanced. These data suggest that Müller cells in the retina may participate in signaling, and this may serve as an extra-neuronal signaling pathway.


2013 ◽  
Vol 114 (9) ◽  
pp. 094901 ◽  
Author(s):  
D. Pugliese ◽  
N. Shahzad ◽  
A. Sacco ◽  
E. Tresso ◽  
A. L. Alexe-Ionescu

2011 ◽  
Vol 217 (1) ◽  
pp. 236-241 ◽  
Author(s):  
G. Kantonis ◽  
T. Stergiopoulos ◽  
A.P. Katsoulidis ◽  
P.J. Pomonis ◽  
P. Falaras

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