scholarly journals Immunogold Staining of Epoxy Resin Sections for Transmission Electron Microscopy (TEM)

2008 ◽  
Vol 2008 (6) ◽  
pp. pdb.prot5015-pdb.prot5015 ◽  
Author(s):  
J. N. Skepper ◽  
J. M. Powell
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Epoxy Resin ◽  
Immunogold Staining ◽  
Transmission Electron

Magnetization, micro-x-ray fluorescence, and transmission electron microscopy studies of low concentrations of nanoscale Fe3O4 particles in epoxy resin

2000 ◽  
Vol 15 (11) ◽  
pp. 2488-2493 ◽  
Author(s):  
A. N. Thorpe ◽  
F. E. Senftle ◽  
M. Holt ◽  
J. Grant ◽  
W. Lowe ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Epoxy Resin ◽  
Magnetization Measurements ◽  
X Ray ◽  
Transmission Electron ◽  
Low Concentrations ◽  
Magnetite Particles ◽  
Spherical Agglomerates ◽  
Photon Source

Magnetization measurements, transmission electron microscopy (TEM), and high-resolution micro-x-ray fluorescence (μ-XRF) using a synchrotron radiation source (Advanced Photon Source) were used to examine Fe3O4 particle agglomerates of nominally 10-nm particles at low concentrations (down to 0.03%) in thick epoxy resin samples. The magnetization measurements showed that at low concentrations (<0.5%) the magnetite particles, although closely packed in the agglomerates, did not interact magnetically. Predicated on a 2-μm sample step scan, the μ-XRF results were compatible with the presence of spherical agglomerates due to magnetostatic attraction, and these ranged in size from 100 to several thousand nanometers, as observed in TEM measurements. At smaller step scans the resolution could be significantly improved. Thus, the synchroton μ-XRF method was very useful in detecting very small concentrations of particles in thick samples and could probably be used to detect particles in amounts as low as 10−16 g.

Mg(OH)2/Epoxy Resin Nanocomposite Prepared by a Novel Method

2005 ◽  
Vol 13 (5) ◽  
pp. 525-527
Author(s):  
Cheng Yiyun ◽  
Cui Ronghui ◽  
He Pingsheng
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Epoxy Resin ◽  
New Method ◽  
Resin Matrix ◽  
Epoxy Resin Matrix ◽  
Transmission Electron ◽  
Micro Emulsion ◽  
Novel Method ◽  
Micro Reactor

This study presents a new method of preparing Mg(OH)2/epoxy resin nanocomposites. An epoxy resin micro-emulsion is taken as a micro-reactor for the formation of Mg(OH)2 nano-crystals. After the reaction, the collected epoxy proved to be a composite with embedded nano-Mg(OH)2. Transmission electron microscopy (TEM) indicated that the Mg(OH)2 nano-crystals were dispersed uniformly in cured epoxy resin matrix.

Demountable polished extra-thin sections and their use in transmission electron microscopy

1981 ◽  
Vol 44 (335) ◽  
pp. 357-359 ◽  
Author(s):  
D. J. Barber
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Epoxy Resin ◽  
Essential Feature ◽  
Heterogeneous Materials ◽  
Glass Slide ◽  
Thin Sections ◽  
Fine Grained ◽  
Transmission Electron ◽  
Polished Side

The advantages of polished ultra-thin sections (PUTS) in the study of very fine-grained materials, such as occur in some meteorites, have been illustrated by Fredriksson et al. (1978) whose technique is based on the earlier work of Beauchamp and WiUiford (1974). An essential feature of such methods for friable and heterogeneous materials is the use of a medium, usually an epoxy resin, to consolidate and partially impregnate them. Normally one polished side of the specimen is bonded to a glass slide during preparation, and the finished PUTS are integral with the slide on completion. PUTS are typically 2-5 microns in thickness.

Cuticular Folds in three Species of Lycopersicon as Observed by Scanning and Transmission Electron Microscopy

1970 ◽  
Vol 28 ◽  
pp. 132-133
Author(s):  
Arthur R. Spurr
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Lycopersicon Esculentum ◽  
Epoxy Resin ◽  
Wild Species ◽  
Transmission Electron ◽  
Scanning Electron

Differences in the architecture of the cuticle of Lycopersicon esculentum, L. peruvianum, and L. pennellii (the cultivated and two wild species of tomato, respectively) were examined by scanning electron microscopy. The cuticle of L. esculentum was also studied by transmission electron microscopy to assist in the interpretation of cuticular folds.For SEM, fresh styles were removed from flowers just prior to anthesis and attached to specimen stubs and promptly observed. For TEM, the material was fixed in glutaraldehyde-Os04, dehydrated in ethanol, embedded in epoxy resin, and section-stained with uranyl and lead acetates. Observations were confined to the upper portion of the style.

Expanding behaviour, structural disorder, regular and random irregular interstratification of 2:1 layer-silicates studied by high-resolution images of transmission electron microscopy

Clay Minerals ◽  
1986 ◽  
Vol 21 (5) ◽  
pp. 827-859 ◽  
Author(s):  
H. Vali ◽  
H. M. Köster
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
High Resolution ◽  
Epoxy Resin ◽  
Clay Minerals ◽  
Central Zone ◽  
Double Layers ◽  
X Rays ◽  
Ammonium Ions ◽  
Transmission Electron

AbstractExpanding and non-expanding layers of interstratified clay minerals have been examined by high-resolution transmission electron microscopy. Permanent expansion of swelling layers under the electron beam was achieved by intercalation of n-alkylammonium ions, especially the octadecylammonium ion. Oriented flakes of clay minerals were prepared by embedding the expanded or non-expanded clay minerals in epoxy resin, followed by centrifugation before hardening of the resin. The minerals were then cut perpendicular to 001 using an ultramicrotome. Crystals of macroscopic trioctahedral vermiculites show homogeneous interplanar distances of 24 Å after intercalation of octadecylammonium ions. Crystals of dioctahedral soil vermiculites often show a central zone with non-expanding 10 Å layers; the outer zone shows a disturbed layer sequence extensively expanded by n-alkylammonium ions. After embedding in epoxy resin, vermiculites show stable 9·2 Å interplanar spacings but smectites expand to 13 Å. Montmorillonites of the Wyoming type show curved stacks of layers. Most of the layer stacks of montmorillonites of the Cheto type are split and disordered aggregates of single layers are formed. Crystals of illites and glauconites are built up of aggregated small stacks of 10 Å layers, the layer stacks consisting of 10 layers. Mostly the boundaries of the layer stacks are parallel to their 001 planes; sometimes low-angle boundaries are found. The dimensions of the layer stacks, ∼ 100 Å thick and some hundreds of Å in plane, are equal to the dimensions of the domains of coherent scattering of X-rays. The border layers between the layer stacks are identical with those 5 to 10% of layers which swell with glycerol or ethylene glycol during X-ray analysis. Some of the layer stacks of illite and glauconite crystals are expanded by octadecylammonium ions within a fortnight. The other stacks show unchanged 10 Å spacings. The different expanding behaviour of different layer stacks reflects the heterogeneity of the layer-charge distribution in the mica clay minerals. K-bentonites show the same expanding behaviour as illites and glauconites but the number of layers expanding with octadecylammonium ions is greater in K-bentonites than in illite crystals. Expanded mixed-layered minerals of the illite-smectite type show a different layer stacking sequence from illites. Random irregular stacking of mica layers with expanded layers are recognized rather than coherent stacks of mica layers. The crystals have a stepped morphology, perhaps effected by translations along the 001 plane. After reaction of the rectorite from Garland County with octadecylammonium ions, the non-expanded mica layers and the expanded smectite-like layers can be distinguished. The heterogeneity of the interlayer charges of the smectite layers is documented by the formation of alkyl double-layers with 17 Å spacings and alkyl triple-layers with 21 Å spaces in irregular sequence. The ‘rectorite’ from the Goto Mine expands nearly homogeneously in comparison with the rectorite from Garland County. After reaction with octadecylammonium ions, interplanar spacings of mostly 31 Å are observed but rarely spacings of 27 Å. The smectite layers of the corrensite from Kaubenheim are expanded by tetradecyl-ammonium ions to 18 Å spacings by formation of alkyl double-layers. A regular 1 : 1 layer structure of 14 Å chlorite layers and expanded 18 Å smectite layers with total spacing of 32 Å can be observed. Muscovite and pyrophyllite are not expandable by n-alkyl-ammonium ions within a fortnight. However, sporadic layers of celadonite crystals are expanded. Generally the 10 Å or 9·2 Å layers extend over the whole crystals of the three minerals. In celadonite crystals, disorder is caused sporadically by interrupted layers or slightly enlarged layer spacings.

Observation Technique of Internal Microstructure of Micro-Powder by Electron Microscopy

2011 ◽  
Vol 412 ◽  
pp. 445-448 ◽  
Author(s):  
Zheng Min Li ◽  
Bing Jiang ◽  
Chen Xi Zhai ◽  
Zhi Wei Chen ◽  
Liu Feng ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Epoxy Resin ◽  
Transmission Electron ◽  
Scanning Electron

Two observation techniques of internal microstructure, including hole and second particle, of micron scale powders have been established and applied to frangible and tough powders. One is that powders were embedded in epoxy resin, sectioned by an ultramicrotome, then observed by using the transmission electron microscopy (TEM) in turn; another is that the fracture of powders were observed by using the scanning electron microscopy (SEM).

Specimen Preparation of Thin Crystalline Fibers for Transmission Electron Microscopy

1990 ◽  
Vol 199 ◽  
Author(s):  
Rosemarie Koch ◽  
Ann F. Marshall
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Epoxy Resin ◽  
Cross Sections ◽  
Specimen Preparation ◽  
Thin Glass ◽  
Alternative Method ◽  
Transmission Electron ◽  
Short Piece ◽  
Transmission Electron Microscopy Cross

ABSTRACTA technique was developed to prepare crystalline fibers of less than 1 mm diameter for transmission electron microscopy. Cross sections were made by casting a short piece of the fiber in an epoxy resin, sectioning the block, and laminating the slices against thin glass discs for stability before dimpling. Longitudinal sections were reinforced in a similar manner. TEM tungsten rings were sometimes used as an alternative method to add stability to the longitudinal sections. The technique was especially developed for Bi-Sr-Ca-Cu-O compounds but is also suitable for other materials.

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