Identification of Differential Genes by Suppression Subtractive Hybridization: IV. Mirror Orientation Selection (MOS)

2008 ◽  
Vol 2008 (8) ◽  
pp. pdb.prot4858-pdb.prot4858
Author(s):  
D. V. Rebrikov
Keyword(s):  
Suppression Subtractive Hybridization ◽  
Subtractive Hybridization ◽  
Orientation Selection ◽  
Mirror Orientation Selection ◽  
Differential Genes

scholarly journals Suppression Subtractive Hybridization and Differential Screening Reveals Endodormancy-associated Expression of an SVP/AGL24-type MADS-box Gene in Lateral Vegetative Buds of Japanese Apricot

2008 ◽  
Vol 133 (5) ◽  
pp. 708-716 ◽  
Author(s):  
Hisayo Yamane ◽  
Yukinobu Kashiwa ◽  
Tomomi Ooka ◽  
Ryutaro Tao ◽  
Keizo Yonemori
Keyword(s):  
Suppression Subtractive Hybridization ◽  
Subtractive Hybridization ◽  
Differential Screening ◽  
Gene Pools ◽  
Mads Box ◽  
Japanese Apricot ◽  
Cell Wall Modification ◽  
Gibberellin Metabolism ◽  
Lateral Buds ◽  
Mirror Orientation Selection

To understand the molecular basis of the endodormancy of buds of perennial plants, we searched for the genes that are expressed preferentially in endodormant lateral buds of the deciduous fruit tree japanese apricot (Prunus mume Sieb. et Zucc.) using suppression subtractive hybridization with mirror orientation selection (SSH/MOS). We generated two SSH/MOS libraries containing gene pools that are expressed preferentially in endodormant buds in comparison with paradormant or ecodormant buds to search for the genes that are upregulated by endodormancy induction or down-regulated by endodormancy release, respectively. Differential screening and sequencing indicated that genes involved in gibberellin metabolism, stress resistance, cell wall modification, and signal transduction, such as transcription factors, are upregulated in endodormant buds. After a further expression survey and full-length cDNA cloning, we found that a gene similar to the SVP/AGL24-type MADS-box transcription factor showed endodormancy-associated expression. Seasonal expression analysis suggested that the SVP/AGL24 homolog in japanese apricot might be involved in endodormancy regulation of its lateral buds.

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