Subcellular Localization of GUS- and GFP-Tagged Proteins in Onion Epidermal Cells

2007 ◽  
Vol 2007 (2) ◽  
pp. pdb.prot4689-pdb.prot4689 ◽  
Author(s):  
A. von Arnim
Keyword(s):  
Subcellular Localization ◽  
Epidermal Cells ◽  
Onion Epidermal Cells

An efficient transient assays system using Agrobacterium-mediated transformation of onion (Allium cepa) epidermal cells

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
Yu-Miao Zhang ◽  
Jun Wang ◽  
Tao Wu
Keyword(s):  
Subcellular Localization ◽  
Protein Interaction ◽  
Protein Interactions ◽  
Epidermal Cells ◽  
Cyclin Dependent Kinase ◽  
Protein Subcellular Localization ◽  
Protein Protein Interactions ◽  
Efficient System ◽  
Protein Protein Interaction ◽  
Onion Epidermal Cells

In this study, the Agrobacterium infection medium, infection duration, detergent, and cell density were optimized. The sorghum-based infection medium (SbIM), 10-20 min infection time, addition of 0.01% Silwet L-77, and Agrobacterium optical density at 600 nm (OD600), improved the competence of onion epidermal cells to support Agrobacterium infection at >90% efficiency. Cyclin-dependent kinase D-2 (CDKD-2) and cytochrome c-type biogenesis protein (CYCH), protein-protein interactions were localized. The optimized procedure is a quick and efficient system for examining protein subcellular localization and protein-protein interaction.

scholarly journals Ice crystal growth of living onion epidermal cells as affected by freezing rates

2018 ◽  
Vol 21 (1) ◽  
pp. 606-617 ◽  
Author(s):  
Duohua Xu ◽  
Huaiwen Wang ◽  
Yanan Wang ◽  
Zhe Zhang
Keyword(s):  
Crystal Growth ◽  
Epidermal Cells ◽  
Ice Crystal ◽  
Onion Epidermal Cells

A simple and effective method for protein subcellular localization using Agrobacterium-mediated transformation of onion epidermal cells

Biologia ◽  
2007 ◽  
Vol 62 (5) ◽  
Author(s):  
Wei Sun ◽  
Ziyi Cao ◽  
Yan Li ◽  
Yanxiu Zhao ◽  
Hui Zhang
Keyword(s):  
Subcellular Localization ◽  
Particle Bombardment ◽  
Fusion Proteins ◽  
Fluorescent Proteins ◽  
Transformed Cells ◽  
Protein Subcellular Localization ◽  
Transformation Protocol ◽  
Agrobacterium Mediated Transformation ◽  
The Mean ◽  
Onion Epidermal Cells

AbstractA modified Agrobacterium-mediated transformation protocol has been successfully used for transient expression of the intrinsically fluorescent proteins and their fusion proteins in onion epidermis. The mean of the transformed cells rate per peel is about 10.5±0.9%, while that of the particle bombardment method is at the range 2.0±0.4%. To compare with the prevailing method of micro-projectile bombardment, the modified Agrobacterium-mediated transformation may provide with higher efficiency and even more simplified manipulability on a lower budget.

Osmotic induction of fluid-phase endocytosis in onion epidermal cells

Planta ◽  
1990 ◽  
Vol 180 (4) ◽  
pp. 555-561 ◽  
Author(s):  
K. J. Oparka ◽  
D. A. M. Prior ◽  
N. Harris
Keyword(s):  
Fluid Phase ◽  
Epidermal Cells ◽  
Fluid Phase Endocytosis ◽  
Onion Epidermal Cells

A facile synthesis of a highly water-soluble and selective fluorescent sensor towards zinc ions derived from β-cyclodextrin based on an unexpected sensing process

RSC Advances ◽  
2015 ◽  
Vol 5 (122) ◽  
pp. 100482-100487 ◽  
Author(s):  
Zengchen Liu ◽  
Weijie Yang ◽  
Yanxia Li ◽  
Fengshou Tian ◽  
Wenping Zhu
Keyword(s):  
Fluorescent Sensor ◽  
Epidermal Cells ◽  
Water Soluble ◽  
Zinc Ions ◽  
Facile Synthesis ◽  
Onion Epidermal Cells

A highly selective and sensitive fluorescent sensor for Zn2+ derived from a β-cyclodextrin derivate was fabricated. Through fluorescence micrograph experiments, the sensor showed an excellent image effect on onion epidermal cells.

scholarly journals Particle bombardment and subcellular protein localization analysis in the aquatic plant Egeria densa

2017 ◽  
Author(s):  
Yasuhide Osaki ◽  
Yutaka Kodama
Keyword(s):  
Subcellular Localization ◽  
Particle Bombardment ◽  
Aquatic Plant ◽  
Higher Plants ◽  
Cell Layer ◽  
Protein Localization ◽  
Epidermal Cells ◽  
Egeria Densa ◽  
Easy Method ◽  
Localization Analysis

Particle bombardment is a powerful and relatively easy method for transient expression of genes of interest in plant cells, especially those that are recalcitrant to other transformation methods. This method has facilitated numerous analyses of subcellular localization of fluorescent fusion protein constructs. Particle bombardment delivers genes to the first layer of plant tissue. In leaves of higher plants, epidermal cells are the first cell layer. Many studies have used the epidermal cell layer of onion bulb (Allium cepa) as the experimental tissue, because these cells are relatively large. However, onion epidermal cells lack developed plastids (i.e., chloroplasts), thereby precluding subcellular localization analysis of chloroplastic proteins. In this study, we developed a protocol for particle bombardment of the aquatic plant Egeria densa, and showed that it is a useful system for subcellular localization analysis of higher plant proteins. E. densa leaflets contain only two cell layers, and cells in the adaxial layer are sufficiently large for observation. The cells in both layers contain well-developed chloroplasts. We fused fluorescent proteins to conventional plant localization signals for the nucleus, cytosol, mitochondria, peroxisome, and chloroplast, and used particle bombardment to transiently express these fusion constructs in E. densa leaves. The plant subcellular localization signals functioned normally and displayed the expected distributions in transiently transformed E. densa cells, and even chloroplastic structures could be clearly visualized.

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