Preparation of Vertical SDS Slab Gels: Simultaneous Casting of Multiple-Gradient Gels

2006 ◽  
Vol 2006 (28) ◽  
pp. pdb.prot4583-pdb.prot4583
Author(s):  
W. R. Stochaj ◽  
T. Berkelman ◽  
N. Laird
Keyword(s):  
Slab Gels

Preparative electrofocusing in agarose slab gels

1982 ◽  
Vol 3 (2) ◽  
pp. 85-89 ◽  
Author(s):  
Walter D. Cantarow ◽  
Calvin A. Saravis ◽  
David V. Ives ◽  
Norman Zamcheck
Keyword(s):  
Slab Gels

Purification of glucosylceramidase by affinity chromatography

1982 ◽  
Vol 60 (11) ◽  
pp. 1025-1031 ◽  
Author(s):  
P. M. Strasberg ◽  
J. A. Lowden ◽  
D. Mahuran
Keyword(s):  
Sodium Dodecyl ◽  
Sodium Taurocholate ◽  
Low Ph ◽  
Affinity Column ◽  
Specific Technique ◽  
Natural Substrate ◽  
Ph Optimum ◽  
Specific Affinity ◽  
Glycol Solution ◽  
Slab Gels

Glucosylceramide:β-glucosidase (glucocerebrosidase, EC 3.2.1.45) has been purified 12 900-fold from human placenta using a specific affinity column. The ligand, glucosyl sphingosine, prepared from glucocerebroside by alkaline hydrolysis, was attached to epoxy-activated Sepharose 6B. The enzyme was applied to the column in citrate–butanol or citrate – ethylene glycol solution at its pH optimum (5.6). No enzyme was bound in the presence of detergent. Glucocerebrosidase was eluted with citrate–taurocholate buffer at low pH or with citrate-taurocholate buffer containing D-gluconolactone at the pH optimum. Citrate–taurocholate solution alone at the pH optimum would not elute the enzyme. The enzyme hydrolyzed both the natural substrate, glucocerebroside, and the artificial substrate, 4-methylumbelliferyl glucopyranoside. Glucocerebrosidase migrated as a single band on 10% sodium dodecyl sulfate–polyacrylamide tube and (or) slab gels, corresponding to a molecular weight of 75 000. It also ran as a single zone of enzyme activity or protein on native gels, composed of 2.2% polyacrylamide – 0.4% agarose containing sodium taurocholate. This is the first reported use of this gel system for the examination of glucocerebrosidase. Overall recovery is 30%. The procedure represents a more rapid and specific technique for purification of glucocerebrosidase than those previously reported.

[10] High-speed automated DNA sequencing in ultrathin slab gels

1996 ◽  
pp. 219-237 ◽  
Author(s):  
Lloyd M. Smith ◽  
Robert L. Brumley ◽  
Eric C. Buxton ◽  
Michael Giddings ◽  
Michael Marchbanks ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
High Speed ◽  
Automated Dna Sequencing ◽  
Slab Gels

Improved phenotyping of apolipoprotein E: application to population frequency distribution

1990 ◽  
Vol 36 (11) ◽  
pp. 1871-1874 ◽  
Author(s):  
J S Hill ◽  
P H Pritchard
Keyword(s):  
Population Studies ◽  
Clinical Laboratory ◽  
Simple Procedure ◽  
Low Density Lipoprotein ◽  
Large Population ◽  
Density Lipoprotein ◽  
Lipid Clinic ◽  
Apo E ◽  
Slab Gels ◽  
Peroxidase Conjugate

Abstract A simple procedure for phenotyping apolipoprotein (apo) E directly from plasma has been developed for use in the lipid clinic laboratory. In this new method, 10 microL of serum or plasma is pretreated with neuraminidase (EC 3.2.1.18), which removes the sialic acid residues from apo E and eliminates additional bands, thereby ensuring correct phenotype assignment. After a rapid delipidation step, the samples are focused in vertical polyacrylamide mini-slab gels and immunoblotted with a polyclonal goat anti-apo E antibody, followed by a Protein G-peroxidase conjugate. The accuracy of this method was confirmed by comparison with the established procedure of phenotyping by isoelectric focusing of delipidated very-low-density lipoprotein. In addition, sera from 203 subjects from Vancouver, selected without conscious bias, were used to determine the local distribution of the apo E alleles. We estimate that the relative frequencies of apo E alleles epsilon 2, epsilon 3, and epsilon 4 in this population are 0.086, 0.761, and 0.153, respectively. The speed and convenience of using minigels make this procedure ideal for clinical laboratory applications and large population studies.

An improved method for arylsulfatase a detection on polyacrylamide slab gels

1978 ◽  
Vol 89 (2) ◽  
pp. 423-429 ◽  
Author(s):  
Paul Manowitz ◽  
Luce Goldstein ◽  
Frank Bellomo
Keyword(s):  
Arylsulfatase A ◽  
Improved Method ◽  
Slab Gels

Analysis of recombinant plasmids using multiple agarose slab gels in series

1984 ◽  
Vol 1 (5) ◽  
pp. 79-83 ◽  
Author(s):  
Claude Hamelin ◽  
Michel Dion ◽  
Jocelyn Yelle
Keyword(s):  
Recombinant Plasmids ◽  
In Series ◽  
Slab Gels
Sign in / Sign up

Export Citation Format

Share Document