Genome-Wide Analysis of Protein-Protein Interactions Using a Two-Hybrid Array: Cloning by Homologous Recombination and High-Throughput Transformation

2006 ◽  
Vol 2006 (23) ◽  
pp. pdb.prot4562-pdb.prot4562
Author(s):  
T. R. Hazbun ◽  
J. P. Miller
Keyword(s):  
Homologous Recombination ◽  
High Throughput ◽  
Protein Interactions ◽  
Protein Protein Interactions ◽  
Genome Wide Analysis ◽  
Genome Wide ◽  
Two Hybrid

scholarly journals Yeast Two-Hybrid Systems and Protein Interaction Mapping Projects for Yeast and Worm

Yeast ◽  
2000 ◽  
Vol 1 (2) ◽  
pp. 88-94 ◽  
Author(s):  
Albertha J. M. Walhout ◽  
Simon J. Boulton ◽  
Marc Vidal
Keyword(s):  
Hybrid Systems ◽  
High Throughput ◽  
Protein Interaction ◽  
Protein Interactions ◽  
Large Scale ◽  
Protein Protein Interactions ◽  
Yeast Two Hybrid ◽  
Systematic Analysis ◽  
Technical Features ◽  
Two Hybrid

The availability of complete genome sequences necessitates the development of standardized functional assays to analyse the tens of thousands of predicted gene products in high-throughput experimental settings. Such approaches are collectively referred to as ‘functional genomics’. One approach to investigate the properties of a proteome of interest is by systematic analysis of protein–protein interactions. So far, the yeast two-hybrid system is the most commonly used method for large-scale, high-throughput identification of potential protein–protein interactions. Here, we discuss several technical features of variants of the two-hybrid systems in light of data recently obtained from different protein interaction mapping projects for the budding yeastSaccharomyces cerevisiaeand the nematodeCaenorhabditis elegans.

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