Preparing Paraffin Tissue Sections for Immunostaining

2006 ◽  
Vol 2006 (1) ◽  
pp. pdb.prot4329
Author(s):  
Ed Harlow ◽  
David Lane
Keyword(s):  
Tissue Sections ◽  
Paraffin Tissue

In Situ Hybridization: Use of 35S-Labeled Probes on Paraffin Tissue Sections

Methods ◽  
2001 ◽  
Vol 23 (4) ◽  
pp. 313-323 ◽  
Author(s):  
Bruce K. Micales ◽  
Gary E. Lyons
Keyword(s):  
In Situ Hybridization ◽  
Tissue Sections ◽  
Paraffin Tissue

Immunohistochemical Heterogeneity of the Endothelium of Blood and Lymphatic Vessels in the Developing Human Liver and in Adulthood

2016 ◽  
Vol 203 (4) ◽  
pp. 243-257 ◽  
Author(s):  
Ivan Nikolić ◽  
Vera Todorović ◽  
Aleksandar Petrović ◽  
Vladimir Petrović ◽  
Marko Jović ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Specific Antigen ◽  
Lymphatic Vessels ◽  
Antigen Expression ◽  
Tissue Sections ◽  
Liver Sinusoids ◽  
Sinusoidal Endothelium ◽  
The Difference ◽  
Human Livers ◽  
Paraffin Tissue

The endothelium of liver sinusoids in relation to the endothelium of other blood vessels has specific antigen expression similar to the endothelium of lymphatic vessels. Bearing in mind that there is no consensus as to the period or intensity of the expression of certain antigens in the endothelium of blood and lymphatic vessels in the liver, the aim of our study was to immunohistochemically investigate the dynamic patterns of the expression of CD31, CD34, D2-40, and LYVE-1 antigens during liver development and in adulthood on paraffin tissue sections of human livers of 4 embryos, 38 fetuses, 6 neonates, and 6 adults. The results show that, in a histologically immature liver at the end of the embryonic period, CD34 molecules are expressed only on vein endothelium localized in developing portal areas, whereby the difference between portal venous branches and CD34-negative central veins belongs to the collecting venous system. In the fetal period, with aging, expression of CD34 and CD31 molecules on the endothelium of central veins and blood vessels of the portal areas increases. Sinusoidal endothelium shows light and sporadic CD34 immunoreactivity in the late embryonic and fetal periods, and is lost in the neonatal and adult periods, unlike CD31 immunoreactivity, which is poorly expressed in the fetal and neonatal periods but is present in adults. The endothelium of sinusoids and lymphatic vessels express LYVE-1, and the endothelium of lymphatic vessels express LYVE-1 and D2-40 but not CD34. Similarity between the sinusoidal and lymphatic endothelium includes the fact that both types are LYVE-1 positive and CD34 negative.

scholarly journals Membrane testosterone binding sites in prostate carcinoma as a potential new marker and therapeutic target: Study in paraffin tissue sections

BMC Cancer ◽  
2005 ◽  
Vol 5 (1) ◽  
Author(s):  
Constantina Dambaki ◽  
Christina Kogia ◽  
Marilena Kampa ◽  
Katherine Darivianaki ◽  
Michael Nomikos ◽  
...  
Keyword(s):  
Prostate Carcinoma ◽  
Therapeutic Target ◽  
Binding Sites ◽  
Tissue Sections ◽  
Paraffin Tissue

Preparing Paraffin Tissue Sections for Staining

2021 ◽  
Vol 2021 (3) ◽  
pp. pdb.prot099663
Author(s):  
Scott J. Rodig
Keyword(s):  
Tissue Sections ◽  
Paraffin Tissue

scholarly journals Piezoelectric Ultrasonic Biological Microdissection Device Based on a Novel Flexure Mechanism for Suppressing Vibration

Micromachines ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 196
Author(s):  
Haibo Huang ◽  
Yifan Pan ◽  
Yan Pang ◽  
Hao Shen ◽  
Xiwei Gao ◽  
...  
Keyword(s):  
Vibration Suppression ◽  
Mouse Tissue ◽  
Design Parameters ◽  
Feed Speed ◽  
Driving Voltage ◽  
Driving Frequency ◽  
Lateral Vibration ◽  
Flexure Mechanism ◽  
Tissue Sections ◽  
Paraffin Tissue

Biological microdissection has a wide range of applications in the field of molecular pathology. The current laser-assisted dissection technology is expensive. As an economical microdissection method, piezoelectric ultrasonic microdissection has broad application prospects. However, the performance of the current piezoelectric ultrasonic microdissection technology is unsatisfactory. This paper aims to solve the problems of the low dissecting precision and excessive wear of the dissecting needle caused by the harmful lateral vibration of the present piezoelectric ultrasonic microdissection device. A piezoelectric ultrasonic microdissection device based on a novel flexure mechanism is proposed. By analyzing the flexure hinge flexibility, the type of flexure beam and the optimal design parameters are determined. Through harmonic response simulation analysis, the newly designed microdissection device with a vibration-suppressing mechanism achieves the best vibration effect when the driving frequency is 28 kHz. Under this driving frequency, the lateral vibration suppression effect is improved by 68% compared to the traditional effect without vibration suppression. Then, based on 3D printing technology, a prototype of a novel microdissection device is produced, and its performance is tested. Experiments on dissecting needle vibration tests show that the flexure mechanism does indeed suppress the lateral vibration of the needle tip. We conducted various tissue dissection experiments on paraffin tissue sections. First, we determine the optimal dissecting parameters (driving voltage, frequency, feed speed, cutting angle) of the new equipment through various parameter dissecting experiments. Then, we adopt these optimal dissecting parameters to perform three kinds of dissecting experiments on mouse tissue paraffin section (liver, lung, bone), dissecting experiments on tissue sections of different thicknesses (3 μm, 4 μm, 5 μm), sampling and extraction experiments on complete tissue. The new device has a better dissecting performance for paraffin tissue sections below a 5 μm thickness and can complete various dissecting tasks. Finally, we compare the wear of the dissecting needles of the new and old devices after the same dissecting tasks. The results prove that the suppression of harmful lateral vibration not only significantly improves the dissecting effect but also increases the service life and durability of the dissecting needle, which is beneficial for reducing the equipment costs.

DNA Analysis (Ploidy) of Molar Pregnancies With Image Analysis on Paraffin Tissue Sections

1993 ◽  
Vol 100 (4) ◽  
pp. 451-455 ◽  
Author(s):  
Ian D. R. Barclay ◽  
Laith Dabbagh ◽  
Jeannet Babiak ◽  
Sibrand Poppema
Keyword(s):  
Image Analysis ◽  
Dna Analysis ◽  
Tissue Sections ◽  
Paraffin Tissue
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