Preparation of Vectors for High-Throughput Screening of Soluble Recombinant Proteins

2006 ◽  
Vol 2006 (1) ◽  
pp. pdb.prot4188 ◽  
Author(s):  
Ting-Fang Wang ◽  
Andrew H.-J. Wang
Keyword(s):  
Recombinant Proteins ◽  
High Throughput ◽  
High Throughput Screening

scholarly journals High-throughput screening of soluble recombinant proteins

2009 ◽  
Vol 11 (7) ◽  
pp. 1714-1719 ◽  
Author(s):  
Yan-Ping Shih ◽  
Wen-Mei Kung ◽  
Jui-Chuan Chen ◽  
Chia-Hui Yeh ◽  
Andrew H.-J. Wang ◽  
...  
Keyword(s):  
Recombinant Proteins ◽  
High Throughput ◽  
High Throughput Screening

scholarly journals High-Throughput Screening Identifies Two Novel Small Molecule Enhancers of Recombinant Protein Expression

Molecules ◽  
2020 ◽  
Vol 25 (2) ◽  
pp. 353
Author(s):  
Jiasong Chang ◽  
Xiaoxu Chen ◽  
Ruolin Wang ◽  
Run Shi ◽  
Xiaogang Wang ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Protein Expression ◽  
Small Molecules ◽  
Small Molecule ◽  
Recombinant Proteins ◽  
High Throughput ◽  
High Throughput Screening ◽  
Histone Deacetylase Inhibitors ◽  
Recombinant Protein Expression ◽  
Expression Levels

As a primary strategy for production of biological drugs, recombinant proteins produced by transient transfection of mammalian cells are essential for both basic research and industrial production. Here, we established a high-throughput screening platform for improving the expression levels of recombinant proteins. In total, 10,011 small molecule compounds were screened through our platform. After two rounds of screening, we identified two compounds, Apicidin and M-344, that significantly enhanced recombinant protein expression. Both of the selected compounds were histone deacetylase inhibitors, suggesting that the two small molecules increased the expression levels of recombinant proteins by promoting histone acetylation. Moreover, both molecules showed low cytotoxicity. Therefore, our findings suggest that these small molecules may have wide applications in the future.

High-throughput screening program for botanical extracts

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
L Hingorani ◽  
NP Seeram ◽  
B Ebersole
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Screening Program ◽  
Botanical Extracts

High throughput screening of microbial biodiversity for the discovery of novel cosmeceutical agents

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
K Georgousaki ◽  
N DePedro ◽  
AM Chinchilla ◽  
N Aliagiannis ◽  
F Vicente ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Microbial Biodiversity

High throughput screening to investigate Brazilian Cerrado biome plant extract bank chemical diversity

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
LS Espindola ◽  
RG Dusi ◽  
KR Gustafson ◽  
J McMahon ◽  
JA Beutler
Keyword(s):  
High Throughput ◽  
Plant Extract ◽  
High Throughput Screening ◽  
Chemical Diversity ◽  
Brazilian Cerrado ◽  
Cerrado Biome
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