Directional Cloning of PCR Products

2006 ◽  
Vol 2006 (1) ◽  
pp. pdb.prot4140
Author(s):  
Gina L. Costa ◽  
Michael P. Weiner
Keyword(s):  
Directional Cloning ◽  
Pcr Products

Directional cloning of native PCR products with preformed sticky ends (Autosticky PCR)

1999 ◽  
Vol 260 (6) ◽  
pp. 569-573 ◽  
Author(s):  
J. Gál ◽  
R. Schnell ◽  
S. Szekeres ◽  
M. Kálmán
Keyword(s):  
Directional Cloning ◽  
Pcr Products ◽  
Sticky Ends

Improvement of TA Cloning Method to Facilitate Direct Directional Cloning of PCR Products

2014 ◽  
Vol 565 ◽  
pp. 3-8 ◽  
Author(s):  
Ji Gang Li ◽  
Guo Ying Han ◽  
Xiu Min Li ◽  
Jiao Jiao Sun ◽  
Ke Jing Song ◽  
...  
Keyword(s):  
Fluorescent Protein ◽  
Pcr Primers ◽  
Expression Vectors ◽  
Green Fluorescent Protein Gene ◽  
Reaction Products ◽  
Major Disadvantage ◽  
Cloning Method ◽  
Directional Cloning ◽  
Pcr Products ◽  
Green Fluorescent

Directional cloning is a prerequisite for the construction of expression vectors in molecular biology laboratories. Although TA cloning is widely used to clone unmodified PCR (polymerase chain reaction) products, a major disadvantage of this technique is that cloning is not directional. Here we reported a novel PCR products cloning vector with one deoxythymidine overhang and one deoxycytidine overhang at two 3'-ends respectively. With the choice of nucleotides of 5'-ends of PCR primers, PCR products can be cloned to this vector both directly and directionally. The feasibility and efficacy of this cloning method were confirmed by using a pET-17b derivative vector and a green fluorescent protein gene (EGFP) and a red fluorescent protein reporter (Ds-Red) gene. This cloning strategy may be useful in the high-throughput construction of expression vectors and could be viewed as an interesting improvement of existing TA cloning method.

A universal method for directional cloning of PCR products based on asymmetric PCR

2009 ◽  
Vol 52 (1) ◽  
pp. 41 ◽  
Author(s):  
Bao-Li Wang ◽  
Yan-Li Jiao ◽  
Xiao-Xia Li ◽  
Fang Zheng ◽  
Hui Liang ◽  
...  
Keyword(s):  
Universal Method ◽  
Asymmetric Pcr ◽  
Directional Cloning ◽  
Pcr Products

scholarly journals Directional cloning of PCR products using exonuclease III

1992 ◽  
Vol 20 (16) ◽  
pp. 4369-4370 ◽  
Author(s):  
Stefan Kaluz ◽  
Konrad Kölble ◽  
Kenneth B.M. Reid
Keyword(s):  
Exonuclease Iii ◽  
Directional Cloning ◽  
Pcr Products

A T-Linker Strategy for Modification and Directional Cloning of PCR Products

PCR Protocols ◽  
2003 ◽  
pp. 475-484
Author(s):  
Robert M. Horton ◽  
Raghavanpillai Raju ◽  
Bianca M. Conti-Fine
Keyword(s):  
Directional Cloning ◽  
Pcr Products

scholarly journals TA-GC cloning: A new simple and versatile technique for the directional cloning of PCR products for recombinant protein expression

PLoS ONE ◽  
2017 ◽  
Vol 12 (11) ◽  
pp. e0186568
Author(s):  
Athanasios Niarchos ◽  
Anastasia Siora ◽  
Evangelia Konstantinou ◽  
Vasiliki Kalampoki ◽  
George Lagoumintzis ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Protein Expression ◽  
Recombinant Protein Expression ◽  
Directional Cloning ◽  
Pcr Products ◽  
Versatile Technique

AT-Linker Strategy for Modification and Directional Cloning of PCR Products

PCR Protocols ◽  
2003 ◽  
pp. 475-483
Author(s):  
Robert M. Horton ◽  
Raghavanpillai Raju ◽  
Bianca M. Conti-Fine
Keyword(s):  
Directional Cloning ◽  
Pcr Products

A series of novel directional cloning and expression vectors for blunt-end ligation of PCR products

2009 ◽  
Vol 32 (3) ◽  
pp. 439-443 ◽  
Author(s):  
Qiong Wu ◽  
Xing Zhong ◽  
Chao Zhai ◽  
Ju Yang ◽  
Xiaomei Chen ◽  
...  
Keyword(s):  
Cloning And Expression ◽  
Expression Vectors ◽  
Directional Cloning ◽  
Pcr Products
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